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A binding site for the T-cell co-receptor CD8 on the α3 domain of HLA-A2

Nature volume 345, pages 41–46 (1990)Cite this article

Abstract

Adhesion measurements between CD8 and 48 point mutants of HLA-A2.1 show that the CD8 α-chain binds to the α3 domain of HLA-A2.1. Three clusters of α3 residues contribute to the binding, with an exposed, negatively charged loop (residues 223–229) playing a dominant role. CD8 binding correlates with cytotoxic T-cell recognition and sensitivity to inhibition by anti-CD8 antibodies. Impaired alloreactive T-cell recognition of an HLA-A2.1 mutant with reduced affinity for CD8 is not restored by functional CD8 binding sites on an antigenically irrelevant class I molecule. Therefore, complexes of CD8 and the T-cell receptor bound to the same class I major histocompatibility complex molecule seem to be necessary for T-cell activation.

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Author information

Author notes

  1. Russell D. Salter

    Present address: Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, 15261, USA

Authors and Affiliations

  1. Department of Cell Biology, Stanford University, Stanford, California, 94305, USA

    Russell D. Salter, Richard J. Benjamin & Peter Parham

  2. Department of Pediatrics, Stanford University, Stanford, California, 94305, USA

    Pamela K. Wesley, Sarah E. Buxton & Alan M. Krensky

  3. Department of Cardiovascular Surgery, Stanford University, Stanford, California, 94305, USA

    Carol Clayberger

  4. Department of Biochemistry and Molecular Biology and Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, 02138, USA

    Thomas P. J. Garrett

  5. Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California, 94143, USA

    Anne M. Norment & Dan R. Littman

  6. Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, California, 94143, USA

    Dan R. Littman

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Salter, R., Benjamin, R., Wesley, P. et al. A binding site for the T-cell co-receptor CD8 on the α3 domain of HLA-A2. Nature 345, 41–46 (1990). https://doi.org/10.1038/345041a0

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