Abstract
NEWLY synthesized major histocompatibility complex (MHC) class I molecules in the endoplasmic reticulum are thought to bind peptides of foreign and endogenous antigens1–4. Several lines of evidence indicate that β-2 microglobulin (β2m) and/or peptide ligand participate in the intracellular transport and surface expression of class I molecules, but the nature of their involvement is still unclear5,6. Here we present evidence that culturing non-mutant cells (fibroblast, thymoma or mastocytoma) with a peptide ligand specific for the Ld class I molecule of the mouse leads to a dramatic (fourfold) and specific induction of Ld surface expression. Surprisingly, this peptide ligand-induced expression of Ld does not result in an increased intracellular association of Ld with β 2m. These findings demonstrate that the previously reported decrease in surface expression of Ld results from its failure to be saturated with endogenous self-peptide ligands. This unique feature of Ld could also contribute to the fact that several virus-specific cytotoxic ? cell responses have been found to be Ld-restricted.
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References
Chen, B. P. & Parham, P. Nature 337, 743–745 (1989).
Bouillot, M. et al. Nature 339, 473–475 (1989).
Nuchtern, J., Bonifacino, J., Biddison, W. & Klausner, R. Nature 339, 223–226 (1989).
Yewdell, J. W. & Bennink, J. R. Science 244, 1072–1075 (1989).
Krangel, M. S., Orr, H. T. & Strominger, J. L. Cell 18, 979–991 (1979).
Townsend, A. et al. Nature 340, 443–448 (1989).
Snary, D., Barnstable, C. J., Bodmer, W. F. & Crumpton, J. Eur. J. Immun. 7, 580–585 (1977).
Sodoyer, R. et al. EMBO J. 3, 879–885 (1984).
Gussow, D. et al. Immunogenetics 25, 313–322 (1987).
Williams, D. B., Swiedler, S. J. & Hart, G. W. J. Cell Biol. 101, 725–734 (1985).
Beck, J. C., Hansen, T. H., Cullen, S. E. & Lee, D. R. J. Immun. 137, 916–923 (1986).
Weis, J. H. & Murre, C. J. exp. Med. 161, 356–365 (1985).
Weis, J. H. & Seidman, J. G. J. Immun. 134, 1999–2003 (1985).
Reddehase, M. J., Rothbard, J. B. & Koszinowski, U. H. Nature 337, 651–655 (1989).
Lurquin, C. et al. Cell 58, 293–303 (1989).
Evans, G. A., Margulies, D. H., Shykind, B., Seidman, J. G. & Ozato, K. Nature 300, 755–757 (1982).
Bjorkman, P. J. et al. Nature 329, 512–518 (1987).
Lee, D. R., Rubocki, R. J., Lie, W.-R. & Hansen, T. H. J. exp. Med. 168, 1719–1739 (1988).
Myers, N. B., Lie, W.-R., Nett, M., Rubocki, R. J. & Hansen, T. H. J. Immun. 142, 2751–2758 (1989).
Kornfeld, R. & Kornfeld, S. A. Rev. Biochem. 54, 631–664 (1985).
Ciavarra, R. & Forman, J. J. exp. Med. 156, 778–790 (1982).
Örn, A. et al. Nature 297, 415–417 (1982).
Reddehase, M. J., Buhring, H.-J. & Koszinowski, U. H. J. Virol. 57, 408–412 (1986).
Oi, V. T., Jones, P. P., Goding, J. W., Herzenberg, L. A. & Herzenberg, L. A. Curr. Top. Microbiol. Immun. 81, 115–129 (1978).
Ozato, K., Hansen, T. H. & Sachs, D. H. J. Immun. 125, 2473–2477 (1980).
Ozato, K., Mayer, N. M. & Sachs, D. H. Transplantation 34, 113–120 (1982).
Hasenkrug, K. J., Cory, J. M. & Stimpfling, J. H. Immunogenetics 25, 136–139 (1987).
Cullen, S. E. & Schwartz, B. D. J. Immun. 117, 136–142 (1976).
Laemmli, U. K. Nature 227, 680–685 (1970).
Chatterjee-Das, S., Lillehoj, E. P., Hernandez, D. M., Coligan, J. E. & Sachs, D. H. Immunogenetics 25, 7–14 (1987).
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Lie, WR., Myers, N., Gorka, J. et al. Peptide ligand-induced conformation and surface expression of the Ld class I MHC molecule. Nature 344, 439–441 (1990). https://doi.org/10.1038/344439a0
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DOI: https://doi.org/10.1038/344439a0
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