Abstract
THE Y chromosome determines maleness in mammals. A Y chromosome-linked gene diverts the indifferent embryonic gonad from the default ovarian pathway in favour of testis differentiation, initiating male development1,2. Study of this basic developmental switch requires the isolation of the testis-determining gene, termed TDF in humans and Tdy in mice. ZFY, a candidate gene for TDF, potentially encodes a zinc-finger protein3, and has two Y-linked homologues, Zfy-1 and Zfy-2, in mice4,5. Although ZFY, Zfy-1 and Zfy-2 seem to map to the sex-determining regions of the human and mouse Y chromosomes, there is no direct evidence that these genes are involved in testis determination. We report here that Zfy-1 but not Zfy-2 is expressed in differentiating embryonic mouse testes. Neither gene, however, is expressed in We/We mutant embryonic testes which lack germ cells. These observations exclude both Zfy-1 and Zfy-2 as candidates for the mouse testis-determining gene.
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References
McLaren, A. Trends Genet. 4, 153–157 (1988).
Goodfellow, P. N. & Darling, S. M. Development 102, 251–258 (1988).
Page, D. C. et al. Cell 51, 1091–1104 (1987).
Mardon, G. et al. Science 243, 78–80 (1989).
Nagamine, C. M., Chan, K., Kozak, C. A. & Lau, Y.-F. Science 243, 80–83 (1989).
Mardon, G. & Page, D. C. Cell 56, 765–770 (1989).
Cattanach, B. M., Pollard, C. E. & Hawkes, S. G. Cytogenetics 10, 318–337 (1971).
Burgoyne, P. S., Levy, E. R. & McLaren, A. Nature 320, 170–172 (1986).
Ashworth, A., Swift, S. & Affara, N. Nucleic Acids Res. 17, 2864 (1989).
Merchant, H. Devl Biol. 44, 1–21 (1975).
McLaren, A. in The Origin and Evolution of Sex (eds Halvorson H. O. & Monroy, A.) 289–300 (Liss, New York, 1985).
Mintz, B. & Russell, E. S. J. exp. Zool. 134, 207–237 (1957).
Cattanach, B. M. Mouse News Lett. 59, 18 (1978).
Burgoyne, P. S., Buehr, M., Koopman, P., Rossant, J. & McLaren, A. Development 102, 443–450 (1988).
Sinclair, A. H. et al. Nature 336, 780–783 (1988).
Palmer, M. S. et al. Nature 342, XXX–XXX (1989).
Monk, M. & McLaren, A. J. Embryol. exp. Morph. 63, 75–84 (1981).
Robertson, E., Bradley, A., Kuehn, M. & Evans, M. Nature 323, 445–448 (1986).
Robertson, E. J., Kaufman, M. H., Bradley, A. & Evans, M. in Cold Spring Harbor Conferences on Cell Proliferation, Vol. 10: Teratocarcinomas and Stem Cells (eds. Silver, L. M., Martin, G. R. & Strickland, S. 647–663 (Cold Spring Harbor Lab, New York, 1983).
Chomczynski, P. & Sacchi, N. Analyt. Biochem. 162, 156–159 (1987).
Melton, D. W., Konecki, D. S., Brennand, J. & Caskey, C. T. Proc. natn. Acad. Sci. U.S.A. 81, 2147–2151 (1984).
Borghese, E. Acta Anat. 36, 185–220 (1959).
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Koopman, P., Gubbay, J., Collignon, J. et al. Zfy gene expression patterns are not compatible with a primary role in mouse sex determination. Nature 342, 940–942 (1989). https://doi.org/10.1038/342940a0
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DOI: https://doi.org/10.1038/342940a0
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