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The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants

Nature volume 342, pages 396–401 (1989)Cite this article

Abstract

The requirement for DNA sequence homology in generalized genetic recombination is greatly relaxed in bacterial mutL,mutS and mutH mutants deficient in mismatch repair. In such mutants, inter-generic recombination occurs efficiently between Escherichia coli and Salmonella typhimurium, which are ∼20% divergent in DNA sequence. This finding has implications for speciation, for regulating recombination between diverged repeated sequences, and for hitherto difficult interspecies hybridizations.

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References

  1. Holliday, R. Genet. Res. 5, 282–304 (1964).

    Google Scholar 

  2. Whitehouse, H. L. K. Nature 199, 1034–1040 (1963).

    ADS  CAS  PubMed  Google Scholar 

  3. Fox, M. S. & Allen, M. Proc. natn. Acad. Sci. USA. 52, 412–419 (1964).

    ADS  CAS  Google Scholar 

  4. Meselson, M. in Heritage from Mendel (ed. Brink, R. A.) 3–16 (University of Wisconsin Press, 1965).

    Google Scholar 

  5. Schneider, W. P., Nichols, B. P. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 78, 2169–2173 (1981).

    ADS  CAS  Google Scholar 

  6. Shen, P. & Huang, H. V. Genetics 112, 441–457 (1986).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Das Gupta, C. & Radding, C. M. Nature 295, 71–73 (1982).

    ADS  CAS  Google Scholar 

  8. Radman, M. in Genetic Recombination (eds Kutcherlapati, R. & Smith, G. R.) 169–192 (American Society for Microbiology, Washington DC, 1988).

    Google Scholar 

  9. Radman, M. Genome 31, 68–73 (1989).

    CAS  PubMed  Google Scholar 

  10. Karimova, G. A., Grigoriev, P. S. & Rybchin, V. N. Molekularnaia Genetika Mikrobiologia i Virusologia 10, 29–34 (1985).

    Google Scholar 

  11. Glickman, B. W. & Radman, M. Proc. natn. Acad. Sci. U.S.A. 77, 1063–1067 (1980).

    ADS  CAS  Google Scholar 

  12. Wagner, R. et al. Cold Spring Harb. Symp. quant. Biol. 49, 611–615 (1984).

    CAS  PubMed  Google Scholar 

  13. Doutriaux, M.-P., Wagner, R. & Radman, M. Proc. natn. Acad. Sci. U.S.A. 83, 2576–2578 (1986).

    ADS  CAS  Google Scholar 

  14. Jones, M., Wagner, R. & Radman, M. Cell 50, 621–626 (1987).

    CAS  PubMed  Google Scholar 

  15. Shen, P. & Huang, H. V. Molec. Gen. Genet 218, 358–360 (1989).

    CAS  PubMed  Google Scholar 

  16. Claverys, J. P. & Lacks, S. A. Microbiol. Rev. 50, 133–165 (1986).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Mejean, V. & Claverys, J. P. Molec. Gen. Genet. 197, 467–471 (1984).

    CAS  PubMed  Google Scholar 

  18. Feinstein, S. I. & Low, K. B. Genetics 113, 13–33 (1986).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Ochman, H. & Wilson, A. C. in Escherichia coli and Salmonella typhimurium (ed. Neidhardt, F. C.) 1649–1654 (American Society for Microbiology, Washington DC, 1987).

    Google Scholar 

  20. Radman, M. & Wagner, R. A. Rev. Genet. 20, 523–538 (1986).

    CAS  Google Scholar 

  21. Modrich, P. A. Rev. Biochem. 56, 435–466 (1987).

    CAS  Google Scholar 

  22. Meselson, M. in The Recombination of Genetic Material (ed. Low, B. K.) 91–113 (Academic, New York, 1988).

    Google Scholar 

  23. Kushner, S. R. et al. UCLA Symp. molec. cell. Biol. 11, 153–159 (1983).

    CAS  Google Scholar 

  24. Hickson, I. D. et al. Molec. Gen. Genet. 190, 265–270 (1983).

    CAS  PubMed  Google Scholar 

  25. Kumura, K. & Sekiguchi, M. J. biol. Chem. 259, 1560–1565 (1984).

    CAS  PubMed  Google Scholar 

  26. Baron, L. S. et al. Bacteriol. Rev. 32, 362–369 (1968).

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Middleton, R. B. & Mojica-a, T. Adv. Genet. 16, 53–79 (1971).

    CAS  PubMed  Google Scholar 

  28. Anderson, P. R. & Roth, J. R. A. Rev. Microbiol. 31, 473–505 (1977).

    CAS  Google Scholar 

  29. Lehner, A. F. & Hill, C. W. Genetics 110, 365–380 (1985).

    CAS  PubMed  PubMed Central  Google Scholar 

  30. Lahue, R. S., Au, K. G. & Modrich, P. Science 245, 160–164 (1989).

    ADS  CAS  PubMed  Google Scholar 

  31. Siddiqi, O. & Fox, M. S. J. molec. Biol. 77, 101–123 (1973).

    CAS  PubMed  Google Scholar 

  32. Laengle-Rouault, F., Maenhaut-Michel, G. & Radman, M. EMBO J. 6, 1121–1127 (1987).

    Google Scholar 

  33. Lam, S. & Roth, J. R. Cell 34, 951–960 (1983).

    CAS  PubMed  Google Scholar 

  34. Eliott, T. & Roth, J. R. Molec. Gen. Genet. 213, 332–338 (1988).

    Google Scholar 

  35. Hartman, P. E., Hartman, Z. & Stahl, R. C. Adv. Genet. 16, 1–34 (1971).

    CAS  PubMed  Google Scholar 

  36. Ebel-Tsipis, J., Fox, M. S. & Botstein, D. J. molec. Biol. 71, 449–469 (1972).

    CAS  PubMed  Google Scholar 

  37. Bishop, D. K., Andersen, J. & Kolodner, R. D. Proc. natn. Acad. Sci. U.S.A. 86, 3713–3717 (1989).

    ADS  CAS  Google Scholar 

  38. Brooks, P. et al. Proc. natn. Acad. Sci. U.S.A. 86, 4425–4429 (1989).

    ADS  CAS  Google Scholar 

  39. Brown, T. C. & Jiricny, J. Cell 54, 705–711 (1988).

    CAS  PubMed  Google Scholar 

  40. Britten, R. J. et al. Proc. natn Acad. Sci. U.S.A. 85, 4770–4774 (1988).

    ADS  CAS  Google Scholar 

  41. Lundblad, V. & Kleckner, N. Genetics 109, 3–19 (1985).

    CAS  PubMed  PubMed Central  Google Scholar 

  42. White, R. & Lalouel, J.-M. Sci. Am. 258, 40–48 (1988).

    CAS  PubMed  Google Scholar 

  43. Weinberg, R. A. Cancer Res. 49, 3713–3721 (1989).

    CAS  PubMed  Google Scholar 

  44. Murray, A. W. & Szostak, J. W. A. Rev. cell. Biol. 1, 289–315 (1985).

    CAS  Google Scholar 

  45. Damagnez, V., Doutriaux, M.-P. & Radman, M. J. Bact. 171, 4494–4497 (1989).

    CAS  PubMed  Google Scholar 

  46. Schaaper, R.M. & Radman, M. EMBO J. 8, 3511–3516 (1989).

    CAS  PubMed  PubMed Central  Google Scholar 

  47. Barton, N. H., Jones, J. S. & Mallet, J. Nature 336, 13–14 (1988).

    ADS  Google Scholar 

  48. Nachman, M. W. & Myers, P. Proc. natn. Acad. Sci. U.S.A. 86, 6666–6670 (1989).

    ADS  CAS  Google Scholar 

  49. Abastado, J. P. et al. Proc. natn. Acad. Sci. U.S.A. 81, 5792–5796 (1984).

    ADS  CAS  Google Scholar 

  50. Maizels, N. Trends Genet. 5, 4–8 (1989).

    CAS  PubMed  Google Scholar 

  51. Radman, M. in Cellular Responses to DNA Damage (eds Friedberg, E. C. & Bridges, B. A.) 287–298 (Liss, New York, 1983).

    Google Scholar 

  52. Thon, G., Baltz, T. & Eisen, H. Genes Dev. 3, 1247–1254 (1989).

    CAS  PubMed  Google Scholar 

  53. Seifert, H. S. et al. Nature 336, 392–395 (1988).

    ADS  CAS  PubMed  Google Scholar 

  54. Bachmann, B. J. in Escherichia coli and Salmonella typhimurium (ed. Neidhardt, F. C.) 807–844 (American Society for Microbiology, Washington DC, 1987).

    Google Scholar 

  55. Kahn, P. L. J. Bact. 96, 205–214 (1968).

    CAS  PubMed  Google Scholar 

  56. Sanderson, K. E. & Stocker, B. A. D. in Escherichia coli and Salmonella typhimurium (ed. Neidhardt, F. C.) 1220–1224 (American Society for Microbiology, Washington DC, 1987).

    Google Scholar 

  57. Shanabruch, W. G., Belau, I. & Walker, G. C. J. Bacteriol. 147, 827–835 (1981).

    CAS  PubMed  PubMed Central  Google Scholar 

  58. Miller, J. H. Experiments in Molecular Genetics (Cold Spring Harbor Laboratory, New York, 1972).

    Google Scholar 

  59. Csonka, L. N. Molec. Gen. Genet. 182, 82–86 (1981).

    CAS  PubMed  Google Scholar 

  60. Schmieger, H. Molec. Gen. Genet. 119, 75–88 (1972).

    CAS  PubMed  Google Scholar 

  61. Low, K. B. Bact. Rev. 36, 587–607 (1973).

    Google Scholar 

  62. Leisinger, T. in Escherichia coli and Salmonella typhimurium (ed. Neidhardt, F. C.) 345–351 (American Society for Microbiology, Washington DC, 1987).

    Google Scholar 

  63. Meselson, M. & Radding, C. M. Proc. natn. Acad. Sci. U.S.A. 72, 358–361 (1975).

    ADS  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Institut Jacques Monod, 2 Place Jussieu, Tour 43, 75251, Paris, Cedex 05, France

    Christiane Rayssiguier & Miroslav Radman

  2. Biology Department, University of Utah, Salt Lake City, Utah, 84112, USA

    David S. Thaler

  3. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, 27709, USA

    Miroslav Radman

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  1. Christiane Rayssiguier
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  2. David S. Thaler
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  3. Miroslav Radman
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Rayssiguier, C., Thaler, D. & Radman, M. The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature 342, 396–401 (1989). https://doi.org/10.1038/342396a0

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