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Forward transport of glycoproteins on leading lamellipodia in locomoting cells

Abstract

IN several types of locomoting cells, active rearward transport of particles on the cell surface has been observed and correlated with motility1-4. No forward transport of particles has previously been reported, however. Here we report rapid forward transport of concanavalin A-coated gold particles on the dorsal surfaces of lamellipodia of fish epidermal keratocytes. These movements are active, not diffusive, and more rapid than either rearward particle transport or the rate of cell locomotion. We observed forward transport in migrating, but not in stationary cells, and could block the movement by treatment with cytochalasin D. These studies demonstrate for the first time that small numbers of glycoproteins can be actively transported on the surface of the cell to the front of the lamellipodium. We suggest that this mechanism transports proteins involved in cell locomotion, such as proteins necessary for adhesion, and could also produce an extensile force.

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References

  1. Abercrombie, M., Heaysman, J. E. M. & Pegrum, S. M. Expl Cell Res. 62, 389–398 (1970).

    Article  CAS  Google Scholar 

  2. Dembo, M. & Harris, A. K. J. Cell Biol. 91, 528–536 (1981).

    Article  CAS  Google Scholar 

  3. Bray, D. Proc. natn. Acad. Sci. U.S.A. 65, 905–910 (1970).

    Article  ADS  CAS  Google Scholar 

  4. Bretscher, M. S. Science 224, 681–686 (1984).

    Article  ADS  CAS  Google Scholar 

  5. Heath, J. P. J. Cell Sci. 60, 331–354 (1983).

    CAS  PubMed  Google Scholar 

  6. Wang, Y.-L. J. Cell Biol. 101, 597–602 (1985).

    Article  CAS  Google Scholar 

  7. Forscher, P. & Smith, S. J. J. Cell Biol. 107, 1505–1516 (1988).

    Article  CAS  Google Scholar 

  8. Fisher, G. W., Conrad, P. A., DeBiasio, R. L. & Taylor, D. L. Cell Motil. 11, 235–247 (1988).

    Article  CAS  Google Scholar 

  9. Gelles, J., Schnapp, B. J. & Sheetz, M. P. Nature 331, 450–453 (1988).

    Article  ADS  CAS  Google Scholar 

  10. Schreiner, G. & Unanue E. R. Adv. Immun. 24, 37–165 (1976).

    Article  CAS  Google Scholar 

  11. Euteneuer, U. & Schliwa, M. Nature 310, 58–61 (1984).

    Article  ADS  CAS  Google Scholar 

  12. Harris, A. K., Stopak, D. & Wild, P. Nature 290, 249–251 (1981).

    Article  ADS  CAS  Google Scholar 

  13. Fukui, Y., Lynch, T. J., Brzeska, H. & Korn, E. D. J. Cell Biol. 107, 471a (1988).

    Article  Google Scholar 

  14. Brzeska, H. Lynch, T. J., & Korn, E. D. J. biol. Chem. 263, 427–435 (1988).

    CAS  PubMed  Google Scholar 

  15. Adams, R. J. & Pollard, T. D. Nature 322, 754–756 (1988).

    Article  ADS  Google Scholar 

  16. Albanesi, J. P. et al. J. biol. Chem. 260, 8649–8652 (1985).

    CAS  Google Scholar 

  17. Bergmann, J. E., Kupfer, A. & Singer, S. J. Proc. natn. Acad. Sci. USA. 80, 1367–1371 (1983).

    Article  ADS  CAS  Google Scholar 

  18. Berg, H. C. Random Walks in Biology (Princeton University Press, 1983).

    Google Scholar 

  19. Kolega, J. J. Cell Biol. 102, 1400–1411 (1986).

    Article  CAS  Google Scholar 

  20. Cooper, M. S. & Schliwa, M. J. Cell Biol. 102, 1384–1399 (1986).

    Article  CAS  Google Scholar 

  21. Sheetz, M. et al. Nature 340, 284–288 (1989).

    Article  ADS  CAS  Google Scholar 

  22. Jacobson, K., Ishihara, A. & Inman, R. A. Rev. Physiol. 49, 163–175 (1987).

    Article  CAS  Google Scholar 

Download references

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Kucik, D., Elson, E. & Sheetz, M. Forward transport of glycoproteins on leading lamellipodia in locomoting cells. Nature 340, 315–317 (1989). https://doi.org/10.1038/340315a0

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