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Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons

Nature volume 339, pages 468–470 (1989)Cite this article

Abstract

INOSITOL 1,4,5-trisphosphate (InsP3) mediates the effects of several neurotransmitters, hormones and growth factors by mobilizing Ca2+ from a vesicular, non-mitochondrial intracellular store1. Many studies have indirectly suggested the endoplasmic reticulum (ER) to be the site of InsP3 action2–6, though some have implicated the plasma membrane7,8 or a newly described smooth surfaced structure, termed the calciosome9. Using antibodies directed against a purified InsP3-receptor glycoprotein10, of relative molecular mass 260,000, in electron microscope immunocytochemical studies of rat cerebellar Purkinje cells, we have now localized the InsP3 receptor to ER, including portions of the rough endoplasmic reticulum, a population of smooth-membrane-bound organelles (smooth ER), a portion of subplas-malemmal cisternae and the nuclear membrane, but not to mitochondria or the cell membrane. These results suggest that in cerebellar Purkinje cells, InsP3-induced intracellular calcium release is not the property of a single organelle, but is effected by specialized portions of both rough and smooth ER, and possibly by other smooth surfaced structures. The present findings are the first immunocytochemical demonstration of an InsP3 receptor within a cell.

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References

  1. Berridge, M. J. A. Rev. Biochem. 56, 159–193 (1987).

    Article  CAS  Google Scholar 

  2. Streb, H., Irvine, R. F., Berridge, M. J. & Schulz, I. Nature 306, 67–69 (1983).

    Article  ADS  CAS  Google Scholar 

  3. Streb, H., Bayerdorffer, E., Haase, W., Irvine, R. F. & Schulz, I. J. Membrane Biol. 81, 241–253 (1984).

    Article  CAS  Google Scholar 

  4. Joseph, S. K. & Williamson, J. R. J. biol. Chem. 261, 14658–14664 (1986).

    CAS  PubMed  Google Scholar 

  5. Muallem, S., Schoeffield, M., Pandol, S. & Sachs, G. Proc. natn. Acad. Sci. U.S.A. 82, 4433–4437 (1985).

    Article  ADS  CAS  Google Scholar 

  6. Bayerdorffer, E., Streb, H., Eckhardt, L., Haase, W. & Schulz, I. J. Membrane Biol. 81, 69–82 (1984).

    Article  CAS  Google Scholar 

  7. Guillemette, G., Balla, T., Baukal, A. J. & Catt, K. J. J. biol. Chem. 263, 4541–4548 (1988).

    CAS  PubMed  Google Scholar 

  8. Kuno, M. & Gardner, P. Nature 326, 301–304 (1987).

    Article  ADS  CAS  Google Scholar 

  9. Volpe, P. et al. Proc. natn. Acad. Sci. U.S.A. 85, 1091–1095 (1988).

    Article  ADS  CAS  Google Scholar 

  10. Supattapone, S., Worley, P. F., Baraban, J. M. & Snyder, S. H. J. biol. Chem. 263, 1530–1534 (1988).

    CAS  Google Scholar 

  11. Palay, S. L. & Chan-Palay, V. Cerebellar Cortex. Cytology and Organization (Springer, New York, 1974).

    Book  Google Scholar 

  12. Rothman, J. E. Science 213, 1212–1219 (1981).

    Article  ADS  CAS  Google Scholar 

  13. Ghosh, T. K., Mullaney, J. M., Tarazi, F. I. & Gill, D. L. Nature (submitted).

  14. Worley, P. F., Baraban, J. M., Colvin, J. S. & Snyder, S. H. Nature 325, 159–161 (1987).

    Article  ADS  CAS  Google Scholar 

  15. Supattapone, S. et al. Proc. natn. Acad. Sci. U.S.A. 85, 8747–8750 (1988).

    Article  ADS  CAS  Google Scholar 

  16. Delfert, D. M., Hill, S., Pershadsingh, H. A., Sherman, W. R. & McDonald, J. M. Biochem. J. 236, 37–44 (1986).

    Article  CAS  Google Scholar 

  17. O'Rourke, F. A., Halenda, S. P., Zavoico, G. & Feinstein, M. B. J. biol. Chem. 260, 956–962 (1985).

    CAS  Google Scholar 

  18. Nairn, A. C., Nichols, R. A., Brady, M. J. & Palfrey, H. C. J. biol. Chem. 262, 14265–14272 (1987).

    CAS  PubMed  Google Scholar 

  19. Nairn, A. C. & Palfrey, H. C. J. biol. Chem. 262, 17299–17303 (1987).

    CAS  PubMed  Google Scholar 

  20. Goelet, P., Castellucci, V. F., Schachter, S. & Kandel, E. R. Nature 322, 419–422 (1986).

    Article  ADS  CAS  Google Scholar 

  21. Berridge, M. Nature 323, 294–295 (1986).

    Article  ADS  CAS  Google Scholar 

  22. Hsu, S.-M., Raine, L. & Fanger, M. J. histochem. Cytochem. 29, 577–580 (1981).

    Article  CAS  Google Scholar 

  23. Milner, T. A., Aoki, C., Rex Sheu, K.-F., Blass, J. P. & Pickel, V. M. J. Neurosci. 7, 3171–3190 (1987).

    Article  CAS  Google Scholar 

  24. Milner, T. A., Joh, T. H. & Pickel, V. M. J. Neurosci. 6, 2585–2603 (1986).

    Article  CAS  Google Scholar 

Download references

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Author notes

  1. Solomon H. Snyder: To whom correspondence should be addressed.

Authors and Affiliations

  1. Departments of Neuroscience, Pharmacology and Molecular Sciences, Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland, 21205, USA

    Christopher A. Ross, Surachai Supattapone & Solomon H. Snyder

  2. Consiglio Nazionale delle Ricerche, Center of Cytopharmacology, Department of Pharmacology, M. S. Raffaele Scientific Institute, University of Milano, Milano, 20132, Italy

    Jacopo Meldolesi & Tomohide Satoh

  3. Division of Neurobiology, Department of Neurology, Cornell University Medical College, 411 E., 69th Street, New York, New York, 10021, USA

    Teresa A. Milner

Authors
  1. Christopher A. Ross
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  2. Jacopo Meldolesi
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  3. Teresa A. Milner
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  4. Tomohide Satoh
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  5. Surachai Supattapone
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  6. Solomon H. Snyder
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Ross, C., Meldolesi, J., Milner, T. et al. Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons. Nature 339, 468–470 (1989). https://doi.org/10.1038/339468a0

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