Abstract
We show that cyclin plays a pivotal role in the control of mitosis. A proteolysis-resistant mutant of cyclin prevents the inactivation of maturation promoting factor and the exit from mitosis both in vivo and in vitro. We have used a fractionated extract to study the activation of MPF by added cyclin protein.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Murray, A. W. & Kirschner, M. W. Nature 339, 275–280 (1989).
Picard, A., Peaucellier, G., Le Bouffant, F., Le Peuch, C. & Doree, M. Dev Biol. 109, 311–320 (1985).
Lee, M. & Nurse, P. Trends Genet. 4, 298–290 (1988).
Dunphy, W. G., Brizuela, L., Beach, D. & Newport, J. Cell 54, 423–431 (1988).
Gautier, J., Norbury, C., Lohka, M., Nurse, P. & Maller, J. Cell 54, 433–439 (1988).
Gerhart, J., Wu, M. & Kirschner, M. W. J. Cell Biol. 98, 1247–1255 (1984).
Cyert, M. S. & Kirschner, M. W. Cell 53, 185–195 (1988).
Lohka, M. & Masui, Y. J. Cell Biol. 98, 1222–1230 (1984).
Hara, K., Tydeman, P. & Kirschner, M. W. Proc. natn. Acad. Sci. U.S.A. 77, 462–466 (1980).
Newport, J. W. & Kirschner, M. W. Cell 37, 731–742 (1984).
Eckstein, F. Agnew. Chem. Int. Ed. Engl. 22, 423–439 (1983).
Dunphy, W. G. & Newport, J. W. J. Cell Biol. 106, 2047–2056 (1988).
Labbe, J. C., Lee, M. G., Nurse, P., Picard, A. & Doree, M. Nature 355, 251–254 (1988).
Booher, R. & Beach, D. EMBO J. 7, 2321–2327 (1988).
Solomon, M., Booher, R., Kirschner, M. & Beach, D. Cell 54, 738–739 (1988).
Goebl, M. & Byers, B. Cell 54, 739–740 (1988).
Hagan, I., Hayles, J. & Nurse, P. J. Cell Sci. 91, 587–595 (1988).
Draetta, G. et al. Cell 56, 829–838 (1989).
Siegel, V. & Walter, P. Cell 52, 39–49 (1988).
Konarska, M. M., Padgett, R. A. & Sharp, P. A. Cell 38, 731–736 (1984).
Pelham, H. R. B. & Jackson, R. J. Eur. J. Biochem. 67, 247–256 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Murray, A., Solomon, M. & Kirschner, M. The role of cyclin synthesis and degradation in the control of maturation promoting factor activity. Nature 339, 280–286 (1989). https://doi.org/10.1038/339280a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/339280a0
This article is cited by
-
Deep transcriptome profiling reveals limited conservation of A-to-I RNA editing in Xenopus
BMC Biology (2023)
-
Analog-sensitive Cdk1 as a tool to study mitotic exit: protein phosphatase 1 is required downstream from Cdk1 inactivation in budding yeast
Chromosome Research (2023)
-
Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
Communications Biology (2022)
-
Decomposition of cell activities revealing the role of the cell cycle in driving biofunctional heterogeneity
Scientific Reports (2021)
-
The G2-to-M transition from a phosphatase perspective: a new vision of the meiotic division
Cell Division (2020)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
