Abstract
THE products of the cellular and retroviral fos genes associate with other nuclear proteins1,2, among them the transcription factor APl/Jun (see ref. 3 for a review). The Fos/Jun complex binds to a specific symmetrical DNA recognition sequence3–9 (termed TRE), thus stimulating transcription of the respective gene3,9–12. Here, we show that two distinct regions in Fos are required for the formation of a Fos/Jun/TRE complex. These are the leucine zipper13, involved in the association with Jun14, and a directly adjacent basic region. Specific amino-acid substitutions in this basic, presumably α-helical, region abolish the interaction of Fos/Jun with the TRE but not the association of the two proteins. The functionally crucial amino acids are located in a region of Fos which is structurally similar to the putative DNA-binding sites in Jun and in the yeast transcriptional activator GCN4 (refs 15 and 16).
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References
Curran, T., Van Beveren, C., Ling, N. & Verma, I. M. Molec. cell. Biol. 4, 167–172 (1985).
Franza, B. R. Jr, Sambucetti, L. C., Cohen, D. R. & Curran, T. Oncogene 1, 213–221 (1987).
Curran, T. & Franza, B. R. Jr Cell 55, 395–397 (1988).
Angel, P. et al. Cell 49, 729–739 (1987).
Lee, W. Mitchell, P. & Tjian, R. Cell 49, 741–752 (1987).
Distel, R. J., Ro, H.-S., Rosen, B. S., Groves, D. L. & Spiegelman, B. M. Cell 49, 835–844 (1987).
Rauscher III, F. J., Sambucetti, L. C., Curran, T., Distel, R. J. & Spiegelman, B. M. Cell 52, 471–480 (1988).
Rauscher III, F. J. et al. Science 240, 1010–1016 (1988).
Chiu, R. et al. Cell 54, 541–552 (1988).
Lucibello, F. C. et al. Oncogene 3, 43–51 (1988).
Schönthal, A., Herrlich, P., Rahmsdorf, H. J. & Ponta, H. Cell 54, 325–334 (1988).
Sassone-Corsi, P., Lamph, W. W., Kamps, M. & Verma, I. M. Cell 54, 553–560 (1988).
Landschulz, W. H., Johnson, P. F. & McKnight, S. L. Science 240, 1759–1764 (1988).
Schuermann, M. et al. Cell 56, 507–516 (1989).
Vingron, M., Nordheim, A. & Muller, R. Onc. Res. 3, 1–7 (1988).
Vogt, P. K., Bos, T. J. & Doolittle, R. F. Proc. natn. Acad. Sci. U.S.A. 84, 3316–3319 (1987).
Mölders, H., Jenuwein, T., Adamkiewicz, J. & Müller, R. Oncogene 1, 377–385 (1987).
Jenuwein, T. & Müller, R. Cell 48, 647–657 (1987).
Nakabeppu, Y., Ryder, K. & Nathans, D. Cell 55, 907–915 (1988).
Halazonetis, T. D., Georgopoulos, K. Greenberg, M. E. Leder, P. Cell 55, 917–924 (1988).
Kouzarides, T. Ziff, E. Nature 336, 646–651 (1988).
Sassone-Corsi, P., Ransone, L. J., Lamph, W. W. & Verma, I. M. Nature 336, 692–695 (1988).
Gamier, J., Osguthorpe, D. J. & Robson, B. J. molec. Biol. 120, 97–120 (1978).
Chou, P. Y. & Fasman, G. D. Biochemistry 13, 222–245 (1974).
Van Beveren, C., Enami, S., Curran, T. & Verma, I. M. Virology 135, 229–234 (1984).
Ryseck, R.-P., Hirai, S. I., Yaniv, M. & Bravo, R. Nature 334, 535–539 (1988).
Barberis, A., Saperti-Furga, G. & Busslinger, M. Cell 50, 347–359 (1987).
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Neuberg, M., Schuermann, M., Hunter, J. et al. Two functionally different regions in Fos are required for the sequence-specific DNA interaction of the Fos/Jun protein complex. Nature 338, 589–590 (1989). https://doi.org/10.1038/338589a0
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DOI: https://doi.org/10.1038/338589a0
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