Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Structure of a phage 434 Cro/DNA complex

Nature volume 335, pages 789–795 (1988)Cite this article

Abstract

Comparison of the crystal structure of a complex of the phage 434 Cro protein and a synthetic DNA operator with the complex of the same operator and the 434 repressor DNA-binding domain shows different DNA conformations in the two structures. Binding of the protein determines the precise conformation of the DNA in each case.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Pabo, C. O. & Sauer, R. T. A. Rev. Biochem. 53, 293–321 (1984).

    Article  CAS  Google Scholar 

  2. Anderson, J. E., Ptashne, M. & Harrison, S. C. Nature 326, 846–852 (1987).

    Article  ADS  CAS  Google Scholar 

  3. Aggarwal, A. K., Rodgers, D., Drottar, M., Ptashne, M. & Harrison, S. C. Science (in the press).

  4. Ptashne, M. A Genetic Switch (Cell Press and Blackwell Scientific, Cambridge, Massachusetts, 1986).

    Google Scholar 

  5. Ohlendorf, D. H., Anderson, W. F., Lewis, M., Pabo, C. O. & Matthews, B. W. J. molec. Biol. 169, 757–769 (1983).

    Article  CAS  Google Scholar 

  6. Sauer, R. T., Yocum, R. R., Doolittle, R. F., Lewis, M. & Pabo, C. O. Nature 298, 447–451 (1982).

    Article  ADS  CAS  Google Scholar 

  7. Mondragon, A., Subbiah, S., Almo, A., Drottar, M. & Harrison, S. C. J. molec. Biol. (in the press).

  8. Wolberger, C. & Harrison, S. C. J. molec. Biol. 196, 951–954 (1987).

    Article  CAS  Google Scholar 

  9. Durbin, R. M. et al. Science 232, 1127–1137 (1986).

    Article  ADS  CAS  Google Scholar 

  10. Mondragon, A., Wolberger, C. & Harrison, S. C. J. molec. Biol. (in the press).

  11. Sussman, J. L., Holbrook, S. R., Church, G. M. & Kim, S. H. Acta. crystallogr. A33, 800–804 (1977).

    Article  ADS  CAS  Google Scholar 

  12. Dickerson, R. E., Weinzierl, J. E. & Palmer, R. A. Acta crystallogr. B24, 997–1003.

  13. Bricogne, G. Acta crystallogr. A132, 832–847 (1976).

    Article  ADS  Google Scholar 

  14. Rossmann, M. G. & Blow, D. M. Acta crystallogr. 16, 39–45 (1973).

    Article  Google Scholar 

  15. Bricogne, G. Acta crystallogr. A130, 395–405 (1974).

    Article  ADS  Google Scholar 

  16. Gaykema, W. P. J. et al. Nature 309, 23–29 (1984).

    Article  ADS  CAS  Google Scholar 

  17. Rossmann, M. G. et al. Nature 317, 145–153 (1985).

    Article  ADS  CAS  Google Scholar 

  18. Hogle, J. M., Chow, M. & Filman, D. J. Science 229, 1358–1365 (1985).

    Article  ADS  CAS  Google Scholar 

  19. Rayment, I., Baker, T. S. & Casper, D. L. D. Acta crystallogr. B39, 505–514 (1983).

    Article  CAS  Google Scholar 

  20. Luo, M. et al. Science 235, 182–191 (1987).

    Article  ADS  CAS  Google Scholar 

  21. Jones, T. A. J. appl. Crystallogr. 11, 268–272 (1978).

    Article  CAS  Google Scholar 

  22. Sheriff, S. & Hendrickson, W. A. Acta crystallogr. A43, 118–1121 (1987).

    Article  CAS  Google Scholar 

  23. Anderson, J. E., Ptashne, M. & Harrison, S. C. Proc. natn. Acad. Sci. U.S.A. 81, 1307–1311 (1984).

    Article  ADS  CAS  Google Scholar 

  24. Koudelka, G. B., Harbury, P., Harrison, S. C. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. (in the press).

  25. Bushman, F. D. thesis, Harvard Univ. (1988).

  26. Bushman, F. D., Anderson, J. E., Harrison, S. C. & Ptashne, M. Nature 316, 651–653 (1985).

    Article  ADS  CAS  Google Scholar 

  27. Wharton, R. P., Brown, E. L. & Ptashne, M. Cell 38, 361–369 (1984).

    Article  CAS  Google Scholar 

  28. Koudelka, G. B., Harrison, S. C. & Ptashne, M. Nature 326, 886–888 (1987).

    Article  ADS  CAS  Google Scholar 

  29. Wharton, R. P. & Ptashne, M. Nature 326, 888–891 (1987).

    Article  ADS  CAS  Google Scholar 

  30. Crawford, J. thesis, Harvard Univ. (1977).

  31. Winkler, F. K., Schutt, C. E. & Harrison, S. C. Acta crystallogr. A35, 901–911 (1979).

    Article  ADS  Google Scholar 

Download references

Author information

Author notes

  1. Cynthia Wolberger

    Present address: Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California, 94143-0448, USA

  2. Yicheng Dong

    Present address: Institute of Biophysics, Academia Sinica, Beijing, People's Republic of China

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts, 02138, USA

    Mark Ptashne

  2. Howard Hughes Medical Institute, 7 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

    Yicheng Dong & Stephen C. Harrison

  3. Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California, 94143-0448, USA

    Yicheng Dong

  4. Institute of Biophysics, Academia Sinica, Beijing, People's Republic of China

    Cynthia Wolberger

Authors
  1. Cynthia Wolberger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yicheng Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mark Ptashne
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Stephen C. Harrison
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wolberger, C., Dong, Y., Ptashne, M. et al. Structure of a phage 434 Cro/DNA complex. Nature 335, 789–795 (1988). https://doi.org/10.1038/335789a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/335789a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing