Abstract
Inositol 1,4,5-trisphosphate (Ins P3) is a second messenger releasing intracellular Ca2+ into the cytosol–5. It has recently been proposed that inositol 1,3,4,5-tetrakisphosphate (Ins P4), which is formed from Ins P3 by Ins P3-3-kinase (ref. 6), acts with Ins P3 as a second messenger by promoting extracellular Ca2+ entry7. It has been suggested that Ins P3 itself can act to stimulate Ca2+ uptake from the extracellular fluid, although a physiological function for Ins P4 was not excluded8,9. Transmembrane currents can now be measured in single cells by voltage clamping under conditions where the intracellular perfusion fluid can be changed several times during individual experiments10,11. We have used this method to test the effects of Ins P3 and Ins P4 on the Ca2+-activated K+ current12,13, and now show that neither Ins P3 alone nor Ins P4 alone can activate a sustained current, whereas Ins P3 and Ins P4 in combination evoke a sustained increase in Ca2+-activated K+ current which is dependent on external Ca2+.
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References
1. Streb, H., Irvine, R. F., Berridge, M. J. & Schulz, I. Nature 306, 67-69 (1983). 2. Streb, H., Bayerdorffer, E., Haase, W., Irvine, R. F. & Schulz, I. /. Membrane Biol. 81, 241-253 (1984). 3. Burgess, G. M. et al. Nature 309, 63-66 (1984). 4. Prentki, M. et al. Nature 309, 562-564 (1984). 5. Berridge, M. J. & Irvine, R. F. Nature 312, 315-321 (1984). 6. Irvine, R. F., Letcher, A. J., Heslop, J. P. & Berridge, M. J. Nature 320, 631-634 (1986). 7. Irvine, R. F. & Moor, R. M. Biochem. J. 240, 917-920 (1986). 8. Kuno, M. & Gardner, P. Nature 326, 301-304 (1987). 9. Llano, I., Marty, A. & Tanguy, J. Pflugers Arch. ges. Physiol. 409, 499-506 (1987). 10. Kimura, J., Noma, A. & Irisawa, H. Nature 319, 596-597 (1986). 11. Jauch, P., Petersen, O. H. & Lauger, P. /. Membrane Biol. 94, 99-115 (1986). 12. Petersen, O. H. & Maruyama, Y. Nature 307, 693-696 (1984). 13. Petersen, O. H. & Findlay, I. Physiol. Rev. 67, 1054-1116 (1987). 14. Iwatsuki, N. & Petersen, O. H. /. Physiol, Lond. 275, 507-520 (1978). 15. Iwatsuki, N. & Petersen, O. H. Pflugers Arch. ges. Physiol. 377, 185-187 (1978). 16. Pearson, G. T. & Petersen, O. H. Pflugers Arch. ges. Physiol 400, 51-59 (1984). 17. Findlay, I. /. Physiol, Lond. 350, 179-195 (1984). 18. Trautmann, A. & Marty, A. Proc. natn. Acad. Sci. U.S.A. 81, 611-615 (1984). 19. Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflugers Arch. ges. Physiol 391, 85-100 (1981). 20. Findlay, I., Dunne, M. J. & Petersen, O. H. /. Membrane Biol 83, 169-175 (1985). 21. Iwatsuki, N. & Petersen, O. H. Biochim. biophys. Acta 819, 249-257 (1985). 22. Siffert, W. & Akkerman, J. W. N. Nature 325, 456-458 (1987). 23. Brass, L. F. & Joseph, S. K. J. biol Chem. 260, 15172-15179 (1985). 24. Irvine, R. F., Letcher, A. J., Lander, D. J. & Berridge, M. J. Biochem. J. 240, 301-304 (1986). 25. Soejima, M. & Noma, A. Pflugers Arch. ges. Physiol. 400, 424-431 (1984). 26. Marty, A., Tan, Y. P. & Trautmann, A. J. Physiol, Lond. 357, 293-325 (1984). 27. Findlay, I. & Petersen, O. H. Pflugers Arch. ges. Physiol 403, 328-330 (1985). 28. Evans, M. G. & Marty, A. /. Physiol, Lond. 378, 437-460 (1986). 29. Maruyama, Y. & Petersen, O. H. /. Membrane Biol. 79, 293-300 (1984). 30. Connolly, T., Bansal, V. S., Bross, T. E., Irvine, R. F. & Majerus, P. W. J. biol Chem. 262, 2146-2149 (1987). 31. Biden, T. J. & Wollheim, C. B. /. biol Chem. 261, 11931-11934 (1986). 32. Zilberman, Y., Howe, L. R., Moore, J. P., Hesketh, T. R. & Metcalf, J. C. EMBO J. 6, 957-962 (1987). 33. Petersen, O. H. Am. J. Physiol. 251, G1-G13 (1986). 34. Petersen, O. H. & Gallacher, D. V. A. Rev. Physiol 50, 65-80 (1988). 35. Suzuki, K., Petersen, C. C. H. & Petersen, O. H. FEES Lett. 192, 307-312 (1985). 36. Trimble, E. R., Bruzzone, R., Meehan, C. J. & Biden, T. J. Biochem. J. 242, 289-292 (1987). 37. Irvine, R. F. & Moor, R. M. Biochem. biophys. Res. Commun. 146, 284-290 (1987). 38. Putney, J. W. Jr Cell Calcium 1, 1-12 (1986). 39. Putney, J. W. Jr Am. J. Physiol. 252, G149-G157 (1987). 40. Michell, R. H. Biochim. biophys. Acta 415, 81-147 (1975).
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Morris, A., Gallacher, D., Irvine, R. et al. Synergism of inositol trisphosphate and tetrakisphosphate in activating Ca2+-dependent K+ channels. Nature 330, 653–655 (1987). https://doi.org/10.1038/330653a0
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DOI: https://doi.org/10.1038/330653a0
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