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Identification of the immunodominant CTL epitope of EGFP in C57BL/6 mice

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References

  1. Rosenzweig M, Connole M, Glickman R, Yue SP, Noren B, DeMaria M et al. Induction of cytotoxic T lymphocyte and antibody responses to enhanced green fluorescent protein following transplantation of transduced CD34(+) hematopoietic cells. Blood 2001; 97: 1951–1959.

    Article  CAS  PubMed  Google Scholar 

  2. Stripecke R, Carmen Villacres M, Skelton D, Satake N, Halene S, Kohn D . Immune response to green fluorescent protein: implications for gene therapy. Gene Ther 1999; 6: 1305–1312.

    Article  CAS  PubMed  Google Scholar 

  3. Andersson G, Illigens BM, Johnson KW, Calderhead D, LeGuern C, Benichou G et al. Nonmyeloablative conditioning is sufficient to allow engraftment of EGFP-expressing bone marrow and subsequent acceptance of EGFP-transgenic skin grafts in mice. Blood 2003; 101: 4305–4312.

    Article  CAS  PubMed  Google Scholar 

  4. Annoni A, Battaglia M, Follenzi A, Lombardo A, Sergi-Sergi L, Naldini L et al. The immune response to lentiviral-delivered transgene is modulated in vivo by transgene expressing antigen presenting cells but not by CD4+CD25+ regulatory T cells. Blood 2007; 110: 1788–1796.

    Article  CAS  PubMed  Google Scholar 

  5. Andersson G, Denaro M, Johnson K, Morgan P, Sullivan A, Houser S et al. Engraftment of retroviral EGFP-transduced bone marrow in mice prevents rejection of EGFP-transgenic skin grafts. Mol Ther 2003; 8: 385–391.

    Article  CAS  PubMed  Google Scholar 

  6. Gambotto A, Dworacki G, Cicinnati V, Kenniston T, Steitz J, Tüting T et al. Immunogenicity of enhanced green fluorescent protein (EGFP) in BALB/c mice: identification of an H2-Kd-restricted CTL epitope. Gene Ther 2000; 7: 2036–2040.

    Article  CAS  PubMed  Google Scholar 

  7. Skelton D, Satake N, Kohn DB . The enhanced green fluorescent protein (eGFP) is minimally immunogenic in C57BL/6 mice. Gene Ther 2001; 8: 1813–1814.

    Article  CAS  PubMed  Google Scholar 

  8. Denaro M, Oldmixon B, Patience C, Andersson G, Down J . EGFP-transduced EL-4 cells from tumors in C57BL/6 mice. Gene Ther 2001; 8: 1814–1815.

    Article  CAS  PubMed  Google Scholar 

  9. Parker KC, Bednarek MA, Coligan JE . Scheme for ranking potential HLA-A2 binding peptides based on independent binding of individual peptide side-chains. J Immunol 1994; 152: 163–175.

    CAS  PubMed  Google Scholar 

  10. Karre K, Ljunggren HG, Piontek G, Kiessling R . Selective rejection of H-2-deficient lymphoma variants suggests alternative immune defence strategy. Nature 1986; 319: 675–678.

    Article  CAS  PubMed  Google Scholar 

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Correspondence to M H M Wauben.

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Han, W., Unger, W. & Wauben, M. Identification of the immunodominant CTL epitope of EGFP in C57BL/6 mice. Gene Ther 15, 700–701 (2008). https://doi.org/10.1038/sj.gt.3303104

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