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Novel gene-transferring scaffolds having a cell adhesion molecule–DNA–apatite nanocomposite surface

Gene Therapy volume 14, pages 1750–1753 (2007)Cite this article

Abstract

A low efficiency has long been the most critical problem of conventional gene-transferring systems using calcium phosphates, and this was successfully improved on by using a laminin–DNA–apatite composite (LD-Ap) layer. The gene-transferring efficiency of the LD-Ap surface was 1–2 orders of magnitude higher than that of a DNA–calcium phosphate composite surface. This is because laminin enhances cell adhesion and spreading, and this provides regions of high DNA concentration between a cell and the LD-Ap surface. The efficiency of gene transfer of the LD-Ap surface was equivalent to, or even higher than that mediated using a commercial lipid-based transfection reagent applied using the manufacture’s recommended optimum conditions. In addition, the gene-transferring efficiency of our system could be controlled by changing the laminin and DNA content in the LD-Ap layer. Moreover, our system is composed of highly safe reagents: apatite, DNA and laminin, all of which are present in the human body. Hence, the LD-Ap surface, which enhances cell attachment on its surface, and mediates a safe, highly efficient and controllable gene transfer, is highly applicable to tissue engineering and gene therapy applications.

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Acknowledgements

We acknowledge the technical support of Mr Kozo Tanabe, Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology. This study was supported by the Foundation Advanced Technology Institute, Japan.

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Authors and Affiliations

  1. Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

    A Oyane & H Tsurushima

  2. Institute for Human Science and Biomedical Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

    A Ito

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  1. A Oyane
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  2. H Tsurushima
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  3. A Ito
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Correspondence to A Oyane.

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Oyane, A., Tsurushima, H. & Ito, A. Novel gene-transferring scaffolds having a cell adhesion molecule–DNA–apatite nanocomposite surface. Gene Ther 14, 1750–1753 (2007). https://doi.org/10.1038/sj.gt.3303041

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