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  • Viral Transfer Technology
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Improved titers of HIV-based lentiviral vectors using the SRV-1 constitutive transport element

Gene Therapy volume 7, pages 1421–1424 (2000)Cite this article

Abstract

The development of lentiviral vectors that use Rev-independent mechanisms of nuclear export for their genomic RNA could facilitate the construction of novel anti-HIV vectors. We have improved the titers of Rev-independent lentiviral vectors having the SRV-1 CTE by mutating the major splice donor and acceptor sites present in the vector and by relocalization of the CTE sequences adjacent to the HIV-1 3′LTR. These two modifications have additive beneficial effects on vector titers and packaging efficiency. Packaging these CTE+ vectors expressing marker genes with a Rev-dependent HIV-1 helper vector yields higher titers than are obtained using a Rev-dependent lentiviral vector.

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  1. Clinical Gene Therapy Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA

    M R Mautino, N Keiser & R A Morgan

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  1. M R Mautino
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  2. N Keiser
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  3. R A Morgan
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Mautino, M., Keiser, N. & Morgan, R. Improved titers of HIV-based lentiviral vectors using the SRV-1 constitutive transport element. Gene Ther 7, 1421–1424 (2000). https://doi.org/10.1038/sj.gt.3301262

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  • DOI: https://doi.org/10.1038/sj.gt.3301262

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