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Establishment of complement-resistant retroviral vector by homologous restriction factor 20 gene

Gene Therapy volume 5, pages 282–285 (1998)Cite this article

Abstract

Homologous restriction factor 20 (HRF20, CD59) is one of the complement regulatory factors. In this study, the complement-resistant retroviral vector, which possesses the HRF20 gene as a selection gene, was constructed and examined. The virus-producing cell, transduced with complement-resistant retroviral vector, was established after complement-dependent selection. NIH3T3 and PK15 cells transduced with this virus-producing cell were suc- cessfully selected by complement-dependent selection, which showed significant expression of the HRF20 antigen. In addition, these cells, transduced with tissue plasminogen activator (tPA) cDNA using complement-resistant retroviral vector, expressed tPA antigen after complement-dependent selection. These findings suggest that complement-resistant retroviral vector can be used for the double transduction of HRF20, as well as other genes.

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Authors and Affiliations

  1. Department of Surgery II, Nagoya University, School of Medicine, Nagoya, Japan

    S Hayashi, T Nagasaka, I Yokoyama & H Takagi

  2. First Department of Internal Medicine, Nagoya University, School of Medicine, Nagoya, Japan

    N Emi

  3. Department of Molecular Biology, Nagoya City University, School of Medicine, Nagoya, Japan

    H Okada

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  1. S Hayashi
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  2. N Emi
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  3. H Okada
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  4. T Nagasaka
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  5. I Yokoyama
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  6. H Takagi
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Hayashi, S., Emi, N., Okada, H. et al. Establishment of complement-resistant retroviral vector by homologous restriction factor 20 gene. Gene Ther 5, 282–285 (1998). https://doi.org/10.1038/sj.gt.3300574

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  • DOI: https://doi.org/10.1038/sj.gt.3300574

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