Abstract
The gene encoding E. coli nitroreductase (NTR) was expressed in the luminal cells of the mammary gland of transgenic mice using the ovine β-lactoglobulin promoter. Treatment of NTR expressing animals with the prodrug CB1954 (5-aziridin-1-yl-2-4-dinitrobenzamide) resulted in a rapid and selective killing of this population of cells whereas the closely associated myoepithelial cells were unaffected. NTR-mediated inducible cell ablation offers a number of advantages over the use of HSV1-tk for the selective killing of cells in vivo.
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Clark, A., Iwobi, M., Cui, W. et al. Selective cell ablation in transgenic mice expressing E. coli nitroreductase. Gene Ther 4, 101–110 (1997). https://doi.org/10.1038/sj.gt.3300367
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DOI: https://doi.org/10.1038/sj.gt.3300367
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