Abstract
The cell type specificity of certain enhancers, competition experiments and the interactions that occur between proteins and enhancer sequences demonstrate that enhancers are the targets of specific factors involved in transcription control1. The 246-base pair BclI-PvuII restriction enzyme fragment of polyoma virus has been shown to include two distinct enhancers, Py A and Py B, composed of several subdomains2–4 which interact with nuclear proteins from mouse fibroblasts5–10. Embryonal carcinoma (EC) cells do not permit polyoma virus infection: both viral transcription and DNA replication are blocked11. Host-range mutants of polyoma virus (EC mutants) capable of overcoming the expression block in EC cells have mutations or sequence rearrangements in their enhancer region12. In an attempt to understand the molecular basis of this host restriction we compared the binding patterns displayed on the viral enhancer sequences by nuclear proteins prepared from EC cells or from fibroblasts. We show that one of the fibroblast factors required for Py A enhancer function, almost undetectable in EC cells, is induced after differentiation of these cells into parietal endoderm, suggesting that this protein is crucial in the regulation of viral gene expression during cellular differentiation, and perhaps more generally in the control of gene expression during early embryonic development.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
1. Serfling, E., Jasin, M. & Schaffner, W. Trends Genet. 1, 224–230 (1985). 2. Herbomel, P., Bourachot, B. & Yaniv, M. Cell 39, 653–662 (1984). 3. Veldman, G. M., Lupton, J. & Kamen, R. Molec. cell. Biol. 5, 649–658 (1985). 4. Hassel, J. A., Muller, W. J. & Mueller, C. R. in Cancer Cells Vol. 4 (eds Botchan, M., Grodzicker, T. & Sharp, P.) 103–113 (Cold Spring Harbor Laboratory, New York, 1986). 5. Piette, J., Kryszke, M.–H. & Yaniv, M. EMBO J. 4, 2675–2685 (1985). 6. Bohnlein, E. & Gruss, P. Molec. cell. Biol. 6, 1401–1411 (1986). 7. Fujimura, F. K. Nucleic Acids Res. 14, 2845–2863 (1986). 8. Ostapchuk, P. et al. Proc. natn. Acad. Sci. U.S.A. 83, 8550–8554 (1986). 9. Piette, J. & Yaniv, M. Nucleic Acids Res. 14, 9595–9611 (1986). 10. Piette, J. & Yaniv, M. EMBO J. 6, 1331–1337 (1987). 11. Kelly, F. & Condamine, H. Biochim. biophys. Acta 651, 105–141 (1982). 12. Amati, P. Cell 43, 561–562 (1985). 13. Wirak, D. O. et al. Molec. cell. Biol. 5, 2924–2935 (1985). 14Strickland, S. & Mahdavi, V. Cell 15, 393–403 (1978). 15. Strickland, S., Smith, K. K. & Marotti, K. R. Cell 21, 347–355 (1980). 16. Fujimura, F. K., Silbert, P. E., Eckhart, W. & Linney, E. J. Virol. 39, 306–312 (1981). 17. Linney, E. & Donerly, S. Cell 35, 693–699 (1983). 18. Wasylyk, C., Imter, J. L., Perez–Mutul, J. & Wasylyk, B. Cell 48, 525–534 (1987). 19. Tang, W. J., Berger, S. L., Triezenberg, S. J. & Folk, W. R. Molec. cell. Biol. 7, 1681–1690 (1987). 20. Sen, R. & Baltimore, D. Cell 47, 921–928 (1986). 21. Herbomel, P., De Crombrugghe, B. & Yaniv, M. in Teratocarcinoma Stem Cells (eds Silver, L. M., Martin, G. R. & StrickJand, S.) 285–294 (Cold Spring Harbor Laboratory, New York, 1983). 22. Linney, E., Davis, B., Overhauser, J., Chao, E. & Fan, H. Nature 308, 470–472 (1984). 23. Borrelli, E., Hen, R. & Chambon, P. Nature 312, 608–612 (1984). 24. Lee, W., Haslinger, A., Karin, M. & Tjian, R. Nature 325, 368–372 (1987). 25. Gachelin, G. Biochim. biophys. Acta 516, 27–60 (1978). 26. Imperiale, M., Kao, H. T., Feldman, L. T., Nevins, J. R. & Strickland, S. Molec. cell. Biol 4, 867–874 (1984). 27. Hen, R., Borrelli, E., Fromental, C., Sassone–Corsi, P. & Chambon, P. Nature 312, 249–251 (1986). 28. Treisman, R. Cell 42, 889–902 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kryszke, MH., Piette, J. & Yaniv, M. Induction of a factor that binds to the polyoma virus A enhancer on differentiation of embryonal carcinoma cells. Nature 328, 254–256 (1987). https://doi.org/10.1038/328254a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/328254a0
This article is cited by
-
Both AP-1 and Cbfa1-like factors are required for the induction of interstitial collagenase by parathyroid hormone
Oncogene (1999)
-
Oncogene jun encodes a sequence-specific trans- activator similar to AP-1
Nature (1988)
-
Induction of proto-oncogene JUN/AP-1 by serum and TPA
Nature (1988)
-
A Harvey-ras responsive transcription element is also responsive to a tumour-promoter and to serum
Nature (1988)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
