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Localization of clathrin light-chain sequences mediating heavy-chain binding and coated vesicle diversity

Nature volume 326, pages 203–205 (1987)Cite this article

Abstract

At least four distinct forms of clathrin light chains are found in mammalian cells1–5. This molecular variability derives from tissue-specific patterns of expression of LCa and LCb genes6. Sequence analysis shows an overall homology of 60% between LCa and LCb and the presence of brain-specific insertion sequences. These findings suggest that the different light chains have both shared and specialized functions6. To address this question we have used a panel of monoclonal antibodies to identify two structurally and functionally distinct regions in the clathrin light-chain sequences. One region (residues 158–208) is exposed in native clathrin structures (triskelions7,8 and coated vesicles) and includes the brain-specific insertion sequences. The second region (residues 93–157), which is cryptic in native clathrin structures, is involved in binding the clathrin heavy chain and contains the region of strongest homology with intermediate filament proteins6.

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Author notes

  1. Frances M. Brodsky & Cynthia J. Galloway

    Present address: Department of Pharmacy, University of California, San Francisco, California, 94143, USA

  2. Kurt Drickamer

    Present address: Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York, 10032, USA

Authors and Affiliations

  1. Becton Dickinson Immunocytometry Systems, Mountain View, California, 94039, USA

    Frances M. Brodsky, Cynthia J. Galloway & Gregory S. Blank

  2. Department of Cell Biology, Stanford Medical School, Stanford, California, 94305, USA

    Antony P. Jackson, Heng-Fong Seow & Peter Parham

  3. Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, 60637, USA

    Kurt Drickamer

Authors
  1. Frances M. Brodsky
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  3. Gregory S. Blank
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  4. Antony P. Jackson
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  5. Heng-Fong Seow
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  6. Kurt Drickamer
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  7. Peter Parham
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Brodsky, F., Galloway, C., Blank, G. et al. Localization of clathrin light-chain sequences mediating heavy-chain binding and coated vesicle diversity. Nature 326, 203–205 (1987). https://doi.org/10.1038/326203a0

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