Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

A complementary DNA clone for a macrophage-lymphocyte Fc receptor

A Corrigendum to this article was published on 18 December 1986

Abstract

Macrophages, granulocytes and many lymphocytes express or secrete receptors for the Fc domain of immunoglobulins (Ig)1. These Fc receptors (FcRs) are heterogeneous and can be distinguished on the basis of their cellular distribution and specificities for different immunoglobulin isotypes. Although their functions are not completely understood, FcRs are known to be involved in triggering various effector cell functions and in regulating differentiation and development of B-cells1–4. One of the best characterized is the mouse macrophage-lymphocyte receptor for IgG1 and IgG2b (ref. 5). On macrophages, this FcR mediates the endocytosis of antibody-antigen complexes via coated pits and coated vesicles6,7, the phagocytosis of Ig-coated particles8, and the release of various inflammatory and cytotoxic agents1. It is possible that the receptor possesses an intrinsic ligand-activated ion channel activity responsible for some of these functions9,10. The IgG1/IgG2b FcR has been isolated and shown to be a transmembrane glycoprotein of relative molecular mass (Mr) 47,000–60,000 (47–60 K) containing four N-linked oligosaccharide chains and a large (>10K) cytoplasmic domain11. It is also immunologically indistinguishable from the murine Ly-17 alloantigen which, in turn, is tightly linked to the Mls lymphocyte activation locus12–14. Here we describe the isolation and characterisation of a complementary DNA clone encoding the whole of the IgG1/IgG2b FcR expressed by the mouse macrophage-like cell line P388D1. The receptor is a member of the immunoglobulin superfamily and, like Ly-17, maps to the distal portion of chromosome 1. cDNA probes detect one or two mRNA species in FcR+ macrophage and B-cell lines, but not in FcR cells or a receptor-deficient variant derived from a FcR+ B-cell line15. Finally, DNA hybridization analysis indicates the receptor gene is partially deleted or rearranged in the FcR variant.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Unkeless, J. C., Fleit, H. & Mellman, I. Adv. Immun. 31, 247–270 (1981).

    Article  CAS  Google Scholar 

  2. Phillips, N. E. & Parker, D. C. J. Immun. 132, 627–632 (1984).

    CAS  PubMed  Google Scholar 

  3. Bijsterbosch, M. K. & Klaus, G. G. B. J. exp. Med. 162, 1825–1836 (1985).

    Article  CAS  Google Scholar 

  4. Daeron, M., Yodoi, J., Neauport-Sautes, C., Moncuit, J. and Fridman, W. H. Eur. J. Immun. 15, 662–667 (1985).

    Article  CAS  Google Scholar 

  5. Mellman, I. & Unkeless, J. C. J. exp. Med. 152, 1048–1069 (1980).

    Article  CAS  Google Scholar 

  6. Ukkonen, P., Lewis, V. A., Marsh, M., Helenius, A. & Mellman, I. J. exp. Med. 163, 952–971 (1986).

    Article  CAS  Google Scholar 

  7. Mellman, I. & Plutner, H. J. Cell Biol. 98, 1170–1176 (1984).

    Article  CAS  Google Scholar 

  8. Mellman, I., Plutner, H., Steinman, R. M., Unkeless, J. C. & Cohn, Z. A. J. Cell Biol. 96, 887–895 (1983).

    Article  CAS  Google Scholar 

  9. Young, J. D.-E., Unkeless, J. C., Young, T. M., Mauro, A. & Cohn, Z. A. Nature 306, 186–189 (1983).

    Article  ADS  CAS  Google Scholar 

  10. Young, J. D.-E., Unkeless, J. C., Kaback, H. R. & Cohn, Z. A. Proc. natn. Acad. Sci. U.S.A. 80, 1636–1640 (1983).

    Article  ADS  CAS  Google Scholar 

  11. Green, S. A., Plutner, H. & Mellman, I. J. Biol. Chem. 260, 9867–9874 (1985).

    CAS  PubMed  Google Scholar 

  12. Holmes, K. L., Palfree, R. G. E., Hämmerling, U. & Morse, H. C., III Proc. natn. Acad. Sci. U.S.A. 82, 7706–7710 (1985).

    Article  ADS  CAS  Google Scholar 

  13. Hibbs, M. L., Hogarth, P. M. & McKenzie, F. C. Immunogenetics 22, 335–348 (1985).

    Article  CAS  Google Scholar 

  14. Kozak, C. A., Davidson, W. F. & Morse, H. C., III Immunogenetics 19, 163–168 (1984).

    Article  CAS  Google Scholar 

  15. Jones, B., Tite, J. P. & Janeway, C. A. Jr J. Immun. 136, 348–356 (1986).

    CAS  PubMed  Google Scholar 

  16. Young, R. A. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 80, 1194–1198 (1983).

    Article  ADS  CAS  Google Scholar 

  17. von Heijne, G. J. molec. Biol. 173, 243–251 (1984).

    Article  CAS  Google Scholar 

  18. Kozak, M. Nucleic Acids Res. 12, 857–872 (1984).

    Article  CAS  Google Scholar 

  19. Mostov, K. E., Freidlander, M. & Blobel, G. Nature 308, 37–43 (1984).

    Article  ADS  CAS  Google Scholar 

  20. Williams, A. F., Barclay, A. N., Clark, M. J. & Gagnon, J. in Gene expression during normal and malignant differentiation. (eds Andersson, L. C., Gahmberg, C. G. & Ekblom, P.) (Academic, 1985).

    Google Scholar 

  21. Hemperly, J. J., Murray, B. A., Edelman, G. M. & Cunningham, B. A. Proc. natn. Acad. Sci. U.S.A. 83, 3037–3041 (1986).

    Article  ADS  CAS  Google Scholar 

  22. D'Eustachio, P., Pravtcheva, D., Marcu, K. & Ruddle, F. H. J. exp. Med. 151, 1545–1550 (1980).

    Article  CAS  Google Scholar 

  23. MacInnes, J. I., Morris, V. L., Flintoff, W. F. & Kozak, C. A. Virology 132, 12–25 (1984).

    Article  CAS  Google Scholar 

  24. Festenstein, H. Transplant. Proceed. 8, 339–342 (1976).

    CAS  Google Scholar 

  25. Ullrich, A. et al. Nature 309, 418–425 (1984).

    Article  ADS  CAS  Google Scholar 

  26. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  27. Dale, R. M. K., McClure, B. A. & Houchins, J. P. Plasmid 13, 31–40 (1985).

    Article  CAS  Google Scholar 

  28. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  Google Scholar 

  29. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: a Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lewis, V., Koch, T., Plutner, H. et al. A complementary DNA clone for a macrophage-lymphocyte Fc receptor. Nature 324, 372–375 (1986). https://doi.org/10.1038/324372a0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/324372a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing