Abstract
Tubulin, the main component of intracellular microtubules, is also a major protein in subcellular membrane preparations1–4 and can interact with biological5–7 and artificial8–12 membranes in vitro. Of particular interest is the association of tubulin with postsynaptic junctional lattices enriched in a polypeptide of relative molecular mass (Mr) 50,000 (50K)13, recently identified as the major subunit of the calmodulin-dependent protein kinase14. Phosphorylation of tubulin with a calmodulin-dependent protein kinase similar to that found in postsynaptic densities inhibits its ability to self-assemble into microtubules in a reversible fashion15. This involves the phosphorylation of residues in its 4K carboxy-terminal region, a domain that seems to regulate its self-assembly16–18. The results presented here suggest that the phosphorylation of tubulin with this kinase enhances its ability to interact with membranes. This effect is reversible.
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References
Marotta, C. A., Stocchi, P. & Gilbert, J. M. Brain Res. 167, 93–106 (1979).
Zisapel, N., Levi, N. & Gozes, I. J. Neurochem. 45, 490–496 (1980).
Estridge, M. Nature 268, 60–63 (1977).
Hargreaves, A. J. & Avila, J. J. Neurochem. 45, 490–496 (1985).
Soifer, D. & Czosnek, H. J. Neurochem. 35, 1128–1136 (1980).
Bernier-Valentin, F. & Rousset, B. J. biol Chem. 257, 7092–7099 (1982).
Bernier-Valentin, F., Aunis, D. & Rousset, B. J. Cell Biol. 97, 209–216 (1983).
Caron, J. M. & Berlin, R. D. J. Cell Biol. 81, 665–671 (1979).
Berlin, R. D., Caron, J. M., Huntley, R., Melmed, R. N. & Oliver, J. M. in Biological Functions of Microtubules and Related Structures (eds Sakai, M., Mohri, H. & Borisy, G. G.) 405–423 (Academic, New York, 1982).
Klausner, R. D., Kumar, N., Weinstein, J. N., Blumenthal, R. & Flavin, M. J. biol. Chem. 256, 5879–5885 (1981).
Kumar, N., Klausner, R. D., Weinstein, J. N., Blumenthal, R. & Flavin, M. J. biol. Chem. 256, 5886–5889 (1981).
Kumar, N., Blumenthal, R., Henkart, M., Weinstein, J. N. & Klausner, R. D. J. biol Chem. 257, 15137–15144 (1982).
Kennedy, M. B., Bennett, M. K. & Erondu, N. E. Proc. natn. Acad. Sci. U.S.A. 80, 7357–7361 (1983).
Walters, B. B. & Matus, A. I. Nature 257, 496–498 (1975).
Wandosell, F., Serrano, L., Hernández, M. A. & Avila, J. J. biol. Chem. 261, 10332–10339 (1986).
Serrano, L., Avila, J. & Maccioni, R. B. Biochemistry 23, 4675–4681 (1984).
Serrano, L. de la Torre, J., Maccioni, R. B. & Avila, J. Proc. natn. Acad. Sci. U.S.A. 81, 5985–5993 (1984).
Serrano, L. Montejo de Garcini, E., Hernández, M. A. & Avila, J. Eur. J. Biochem. 153, 595–600 (1986).
Weingarten, M., Lockwood, A., Hwo, S. & Kirschner, M. W. Proc. natn. Acad. Sci. U.S.A. 72, 1858–1862 (1975).
Bradford, M. M. Analyt. Biochem. 72, 248–254 (1976).
Roth, J. Tech. Immunochem. 1, 108–133 (1982).
Helenius, A. & Simons, K. Proc. natn. Acad. Sci. U.S.A. 74, 529–532 (1977).
Shelanski, M. L., Gaskin, F. & Cantor, C. R. Proc. natn. Acad. Sci. U.S.A. 70, 765–768 (1973).
Arce, C. A., Rodriguez, J. A., Barra, H. & Caputto, R. Eur. J. Biochem. 59, 145–149 (1975).
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Hargreaves, A., Wandosell, F. & Avila, J. Phosphorylation of tubulin enhances its interaction with membranes. Nature 323, 827–828 (1986). https://doi.org/10.1038/323827a0
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DOI: https://doi.org/10.1038/323827a0
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