Abstract
The chicken adult β-globin gene is one of the more intensively investigated developmentally regulated loci in higher eukaryotes. Detailed molecular analysis of the locus1,2 allows precise examination of the chromosomal changes that occur on activation of the gene during erythroid maturation. The best studied of these changes are the acquisition of DNase I hypersensitivity3,4, developmentally correlated alteration of CpG-specific cytosine methylation patterns5,6 and in vitro assembly of erythroid-specific protein complexes 5′ to the gene that mimics in vivo creation of the 5′ DNase I hypersensitive ‘region’ lying 60 to 260 nucleotides 5′ to the β-globin cap site in red blood cell chromatin7–9. Here we demonstrate that proximal β-globin DNA sequences lying >112 base pairs (bp) 5′ to the cap site are not involved in determining the erythroid-specific induction characteristics of this gene in transient expression assays, whereas an enhancer sequence within a 300-bp PvuII fragment lying ∼400 nucleotides 3′ to the polyadenylation signal is intimately involved in determining the erythroid cell specificity and correct time of induction of β-globin transcription during red cell maturation.
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Choi, OR., Engel, J. A 3′ enhancer is required for temporal and tissue-specific transcriptional activation of the chicken adult β-globin gene. Nature 323, 731–734 (1986). https://doi.org/10.1038/323731a0
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DOI: https://doi.org/10.1038/323731a0
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