Abstract
The ABC excision nuclease of Escherichia coll is an ATP-depen-dent DNA repair enzyme composed of three protein subunits, UvrA, UvrB and UvrC. The DNA sequences of all three genes have been reported1–3. UvrA, the component that binds directly to the DNA, and UvrB, which attaches itself to the UvrA-DNA complex, both contain consensus sequences thought to be diagnostic of ATP-binding sites4, although the UvrC sequence does not. We now report that a computer analysis of the UvrA sequence has revealed an unusual series of internal duplications centering around putative metal-binding sites which may be involved in the interaction with DNA. We also find a strong evolutionary relationship to a family of prokaryotic membrane-associated active-transport proteins.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Husain, I., Van Houten, B., Thomas, D. C. & Sancar, A. J. biol. Chem. 261, 4895–4901 (1986).
Arikan, E., Kulkarni, M. S., Thomas, D. C. & Sancar, A. Nucleic Acids Res. 14, 2637–2650 (1986).
Sancar, G. B., Sancar, A. & Rupp, W. D. Nucleic Acids Res. 12, 4593–4608 (1984).
Walker, J. E., Saraste, M., Runswick, M. J. & Gay, N. J. EMBO J. 1, 945–951 (1982).
Miller, J., McLachlan, A. D. & Klug, A. EMBO J. 4, 1609–1614 (1985).
Berg, J. M. Science 232, 485–487 (1986).
Hobson, A. C., Weatherwax, R. & Ferro-Luzzi Ames, G. Proc. natn. Acad. Sci. U.S.A. 81, 7333–7337 (1984).
Gilson, E., Higgins, C. F., Hofnung, M., Ferro-Luzzi Ames, G. & Nikaido, H. J. biol. Chem. 257, 9915–9918 (1982).
Higgins, C. F., Hiles, I. D., Whalley, K. & Jamieson, D.J. EMBO J. 4, 1033–1040 (1985).
Surin, B. P., Rosenberg, H. & Cox, G. J. Bact. 161, 189–198 (1985).
Feng, D.-F., Johnson, M. S. & Doolittle, R. F. J. molec. Evol. 21, 112–125 (1985).
Doolittle, R. F. in Protein Engineering(ed. Inouye, M.) (Academic, New York, in the press).
Gilson, E., Nikaido, H. & Hofnung, M. Nucleic Acids Res. 10, 7449–7458 (1982).
Dunn, J. J. & Studier, F. W. J. J. molec. Biol. 148,303–330 (1981).
Burton, Z. F., Gross, C. A., Watanabe, K. K. & Burgess, R. R. Cell 32, 335–349 (1983).
Ferro-Luzzi Ames, G. Curr. Topics Membrane Transport 23, 103–119 (1985).
Sancar, A. & Rupp, W. D. Cell 33, 249–260 (1983).
Van Duin, M. et al. cell 44, 913–923 (1986).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Doolittle, R., Johnson, M., Husain, I. et al. Domainal evolution of a prokaryotic DNA repair protein and its relationship to active-transport proteins. Nature 323, 451–453 (1986). https://doi.org/10.1038/323451a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/323451a0
This article is cited by
-
Improving the acetic acid tolerance and fermentation of Acetobacter pasteurianus by nucleotide excision repair protein UvrA
Applied Microbiology and Biotechnology (2018)
-
The Nobel Prize in Chemistry 2015: Exciting discoveries in DNA repair by Aziz Sancar
Science China Life Sciences (2016)
-
Structure and mechanism of the UvrA–UvrB DNA damage sensor
Nature Structural & Molecular Biology (2012)
-
A Protein Related to Eucaryal and Bacterial DNA-Motor Proteins in the Hyperthermophilic Archaeon Sulfolobus acidocaldarius
Journal of Molecular Evolution (1997)
-
Tracing the spread of fibronectin type III domains in bacterial glycohydrolases
Journal of Molecular Evolution (1994)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
