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Diversity of murine gamma genes and expression in fetal and adult T lymphocytes

Abstract

The search for the genes encoding the T-cell receptor α and β chains revealed a third gene, Tγ (ref. 1), which shares with the Tα (refs 2–7) and Tβ (refs 8–15) genes a number of structural features, including somatic rearrangement during T-cell development. Tγ gene expression appears to be unnecessary in some mature T cells16,17 and is at its greatest in fetal thymocytes18,19, encouraging speculation that Tγ has a role in T-cell development and may be involved in the recognition of polymorphic major histocompatibility complex (MHC) products during thymic education20,21. One argument against the participation of Tγ in such a process has been its apparently limited diversity, due to the small number of gene segments available for rearrangement1,22. We here describe the identification of additional Tγ V-gene segments and demonstrate that they can be rearranged to previously identified J- and C-gene segments and are expressed in fetal thymocytes. In addition we describe a variety of patterns of Tγ mRNA processing which may be significant for Tγ gene regulation.

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References

  1. Saito, H. et al. Nature 309, 757–762 (1984).

    Article  ADS  CAS  Google Scholar 

  2. Saito, H. et al. Nature 312, 36–39 (1984).

    Article  ADS  CAS  Google Scholar 

  3. Chien, Y-h. et al. Nature 312, 31–35 (1984).

    Article  ADS  CAS  Google Scholar 

  4. Arden, B., Klotz, J. L., Siu, G. & Hood, L. E. Nature 316, 783–787 (1985).

    Article  ADS  CAS  Google Scholar 

  5. Hayday, A. C. et al. Nature 316, 828–832 (1985).

    Article  ADS  CAS  Google Scholar 

  6. Winoto, A., Mjolsness, S. & Hood, L. Nature 316, 832–836 (1985).

    Article  ADS  CAS  Google Scholar 

  7. Yoshikai, Y. et al. Nature 316, 837–840 (1985).

    Article  ADS  CAS  Google Scholar 

  8. Yanagi, Y. et al. Nature 308, 145–149 (1984).

    Article  ADS  CAS  Google Scholar 

  9. Hedrick, S. M., Cohen, D. I., Nielsen, E. A. & Davis, M. M. Nature 308, 149–153 (1984).

    Article  ADS  CAS  Google Scholar 

  10. Hedrick, S. M., Nielsen, E. A., Kavaler, J., Cohen, D. I. & Davis, M. M. Nature 308, 153–158 (1984).

    Article  ADS  CAS  Google Scholar 

  11. Chien, Y-h., Gascoigne, N., Kavaler, J., Lee, N. E. & Davis, M. M. Nature 309, 322–326 (1984).

    Article  ADS  CAS  Google Scholar 

  12. Siu, G. et al. Cell 37, 393–401 (1984).

    Article  CAS  Google Scholar 

  13. Mallissen, M. et al. Cell 37, 1101–1110 (1984).

    Article  Google Scholar 

  14. Gascoigne, N., Chien, Y-h., Becker, D., Kavaler, J. & Davis, M. M. Nature 310, 387–391 (1984).

    Article  ADS  CAS  Google Scholar 

  15. Siu, G. et al. Nature 311, 344–350 (1984).

    Article  ADS  CAS  Google Scholar 

  16. Kranz, D. M. et al. Nature 313, 752–755 (1985).

    Article  ADS  CAS  Google Scholar 

  17. Heilig, J. S. et al. Nature 317, 68–70 (1985).

    Article  ADS  CAS  Google Scholar 

  18. Raulet, D. H., Garman, R. D., Saito, H. & Tonegawa, S. Nature 314, 103–107 (1985).

    Article  ADS  CAS  Google Scholar 

  19. Snodgrass, H. R., Dembic, Z., Steinmetz, M. & vonBoehmer, H. Nature 315, 232–233 (1985).

    Article  ADS  CAS  Google Scholar 

  20. Zinkernagel, R. M. Immun. Rev. Rev. 42, 224–270 (1978).

    Article  CAS  Google Scholar 

  21. Bevan, M. J. & Fink, P. J. Immun. Rev. 42, 3–19 (1978).

    Article  CAS  Google Scholar 

  22. Hayday, A. C. et al. Cell 40, 259–269 (1985).

    Article  CAS  Google Scholar 

  23. Reilly, E. B., Kranz, D. M., Tonegawa, S. & Eisen, H. N. Nature 321, 878–880 (1986).

    Article  ADS  CAS  Google Scholar 

  24. Iwamoto, A. et al. J. exp. Med. 163, 1203–1212 (1986).

    Article  CAS  Google Scholar 

  25. Dialynas, D. P. et al. Proc. natn. Acad. Sci. U.S.A. 83, 2619–2623 (1986).

    Article  ADS  CAS  Google Scholar 

  26. Southern, E. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  27. Lefranc, M.-P. & Rabbitts, T. H. Nature 316, 464–466 (1985).

    Article  ADS  CAS  Google Scholar 

  28. Quetermous, T. et al. Science 231, 252–255 (1986).

    Article  ADS  Google Scholar 

  29. Jenkinson, E. J., Franchi, L., Kingston, R. & Owen, J. J. T. Eur. J. Immun. 12, 583–587 (1982).

    Article  CAS  Google Scholar 

  30. Lo, D. & Sprent, J. Nature 319, 672–675 (1986).

    Article  ADS  CAS  Google Scholar 

  31. Sharp, P. A. in The Aderoviruses (ed. Ginsberg, H. S.) 173–204 (Plenum, New York, 1984).

    Book  Google Scholar 

  32. Laski, F. A., Rio, D. C. & Rubin, G. M. Cell 44, 7–19 (1986).

    Article  CAS  Google Scholar 

  33. Rio, D. C., Laski, F. A. & Rubin, G. M. Cell 44, 21–32. 28 (1986).

    Article  CAS  Google Scholar 

  34. ChirgWin, J. M., Przybyla, A. E., Macdonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979).

    Article  CAS  Google Scholar 

  35. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  36. Gubler, U. & Hoffman, B. J. Gene 25, 263–269 (1983).

    Article  CAS  Google Scholar 

  37. Huynh, T. V., Young, R. A. & Davis, R. W. in DNA Cloning: A Practical Approach (ed. Glover, D.) 49–78 (IRL, Oxford, 1984).

    Google Scholar 

  38. Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  Google Scholar 

  39. Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).

    Article  ADS  CAS  Google Scholar 

  40. Nakauchi, H., Ohno, I., Kim, M., Okumura, K. & Tada, T. J. Immun. 132, 88–94 (1984).

    CAS  PubMed  Google Scholar 

  41. Pierres, A., Schmitt-Verhulst, A. M., Bufesne, M., Golstein, P. & Pierres, M. Scand. J. Immun. 15, 619–625 (1982).

    Article  CAS  Google Scholar 

  42. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, New York).

  43. Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. mol. Biol. 113, 237–256 (1977).

    Article  CAS  Google Scholar 

  44. Reilly, E. B., Reilly, R. M., Breyer, R. M., Saver, R. T. & Eisen, H. N. J. Immun. 133, 471–475 (1984).

    CAS  PubMed  Google Scholar 

  45. Studencki, A. B. & Wallace, R. B. DNA 3, 7–15 (1984).

    Article  CAS  Google Scholar 

  46. Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).

    Article  ADS  CAS  Google Scholar 

  47. Breathnach, R. & Chambon, P. A. Rev. Biochem. 50, 349–383 (1981).

    Article  CAS  Google Scholar 

  48. Traunecker, A. et al. EMBO J. 5, 1584–1594 (1986).

    Article  Google Scholar 

  49. Rupp, F., Frech, G., Hengartner, H., Zinkernagel, R. M. & Joho, R, Nature 321, 876–878 (1986).

    Article  ADS  CAS  Google Scholar 

  50. Garman, R., Doherty, P. J. & Raulet, D. H. Cell 45, 733–742 (1986).

    Article  CAS  Google Scholar 

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Heilig, J., Tonegawa, S. Diversity of murine gamma genes and expression in fetal and adult T lymphocytes. Nature 322, 836–840 (1986). https://doi.org/10.1038/322836a0

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