Abstract
Activation of the T-DNA transfer process of Agrobacterium by the plant signal molecule acetosyringone generates a single-stranded, unipolar, linear T-DNA molecule (T-strand)—a potential conjugative intermediate in the transfer of the T-DNA to plant cells. Acetosyringone induction also leads to S1 nuclease-sensitive sites at the Ti plasmid T-DNA borders, and other molecular changes associated with the Ti plasmid T-DNA sequences, which may correspond to specific steps of T-strand synthesis.
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References
Gheysen, G., Dhaese, P., Van Montagu, M. & Schell, J. in Advances in Plant Gene Research Vol. 2 (eds Hohn, B. & Dennis, E. S.) 11–47 (Springer, Vienna, 1985).
Zambryski, P., Depicker, A., Kruger, K. & Goodman, H. J. molec. appl. Genet. 1, 361–370 (1982).
Yadav, N. S., Vanderleyden, J., Bennett, D. R., Barnes, W. M. & Chilton, M.-D. Proc. natn. Acad. Sci. U.S.A 79, 6322–6326 (1982).
Simpson, R. B. et al. Cell 29, 1005–1014 (1982).
Holsters, M. et al. Molec. gen. Genet. 190, 35–41 (1983).
Zambryski, P. et al. EMBO J. 2, 2143–2150 (1983).
de Framond, A. J., Barton, K. A. & Chilton, M.-D. Bio/ technology 1, 262–269 (1983).
Bevan, M. Nucleic Acids Res. 12, 8177–8721 (1984).
An, G., Watson, B. D., Stachel, S., Gordon, M. P. & Nester, E. W. EMBO J. 4, 277–284 (1985).
Stachel, S. E. & Nester, E. W. EMBO J. 5, 1445–1454 (1986).
Douglas, C. J., Staneloni, R. J., Rubin, R. A. & Nester, E. W. J. Bact. 161, 850–860 (1985).
Stachel, S. E., Nester, E. W. & Zambryski, P. C. Proc. natn. Acad. Sci. U.S.A 83, 379–383 (1986).
Stachel, S. E. & Zambryski, P. Cell (in the press).
Stachel, S. E., Messens, E., Van Montagu, M. & Zambryski, P. Nature 318, 624–629 (1985).
Wang, K., Herrera-Estrella, L., Van Montagu, M. & Zambryski, P. Cell 38, 455–462 (1984).
Peralta, E. G. & Ream, L. W. Proc. natn. Acad. Sci. U.S.A 82, 5112–5116 (1985).
Joos, H. et al. Cell 32, 1057–1067 (1983).
Shaw, C. H., Watson, M. D., Carter, G. H. & Shaw, C. H. Nucleic Acids Res. 12, 6031–6041 (1984).
Koukoliková-Nicola, Z. et al. Nature 313, 191–196 (1985).
Southern, E. M. J. molec. Biol. 98, 503–518 (1975).
Bourgeois, S. Meth. Enzym. 21, 491–500 (1971).
Vogt, V. Eur. J. Biochem. 33, 192–200 (1973).
Nishigaki, K., Kaneko, Y., Wakuda, H., Husimi, Y. & Tanaka, T. Nucleic Acids Res. 13, 5747–5760 (1985).
Huang, W. M. & Lehman, I. R. J. biol. Chem. 247, 3139–3146 (1972).
Chase, J. W. & Richardson, C. C. J. biol. Chem. 249, 4545–4561 (1974).
Lilley, D. M. J. Nucleic Acids Res. 9, 1271–1289 (1981).
Panayotatos, N. & Wells, R. D. Nature 289, 466–470 (1981).
Dressler, D. & Potter, H. A. Rev. Biochem. 51, 727–761 (1982).
Willetts, N. & Wilkins, B. Microbiol. Rev. 48, 24–41 (1984).
Thompson, R., Taylor, L., Kelly, K., Everett, R. & Willetts, N. EMBO J. 3, 1175–1180 (1984).
Manning, P. A. & Achtman, M. in Bacterial Outer Membranes (ed. Inouye, M.) 409–447 (Wiley, New York, 1979).
Douglas, C. J., Halperin, W. & Nester, E. W. J. Bact. 152, 1265–1275 (1982).
Dhaese, P., De Greve, H., Decraemer, H., Schell, J. & Van Montagu, M. Nucleic Acids Res. 7, 1837–1849 (1979).
Maniatis, T., Fritsch, E. F. & Sambrook, J., Molecular Cloning, a laboratory Manual (Cold Spring Harbor Laboratories, New York, 1982).
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Stachel, S., Timmerman, B. & Zambryski, P. Generation of single-stranded T-DNA molecules during the initial stages of T-DNA transfer from Agrobacterium tumefaciens to plant cells. Nature 322, 706–712 (1986). https://doi.org/10.1038/322706a0
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DOI: https://doi.org/10.1038/322706a0
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