Abstract
The anterior pituitary gland, which is derived from a common primordium originating in Rathke's pouch, contains phenotypically distinct cell types, each of which express discrete trophic hormones: adrenocorticotropic hormone (ACTH), thyroid-stimulating hormone (TSH), prolactin, growth hormone, and follicle stimulating hormone (FSH)/luteinizing hormone (LH) (reviewed in ref. 1). The structurally related prolactin and growth hormone genes, which are evolutionarily derived from a single primordial gene2, are expressed in discrete cell types—lactotrophs and somatotrophs, respectively—with their expression virtually limited to the pituitary gland1. The pituitary hormones exhibit a temporal pattern of developmental expression with rat growth hormone and prolactin characteristically being the last hormones expressed3–8. The reported co-expression of these two structurally related neuroendocrine genes within single cells prior to the appearance of mature lactotrophs, in a subpopulation of mature anterior pituitary cells, and in many pituitary adenomas1,6–9 raises the possibility that the prolactin and growth hormone genes are developmentally controlled by a common factor(s). We now report the identification and characterization of nucleotide sequences in the 5′-flanking regions of the rat prolactin and growth hormone genes, respectively, which act in a position- and orientation-independent fashion to transfer cell-specific expression to heterologous genes. At least one putative trans-acting factor required for the growth hormone genomic sequence to exert its effects is apparently different from those modulating the corresponding enhancer element(s) of the prolactin gene because a pituitary ‘lactotroph’ cell line producing prolactin but not growth hormone selectively fails to express fusion genes containing the growth hormone enhancer sequence.
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Nelson, C., Crenshaw, E., Franco, R. et al. Discrete cis-active genomic sequences dictate the pituitary cell type-specific expression of rat prolactin and growth hormone genes. Nature 322, 557–562 (1986). https://doi.org/10.1038/322557a0
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DOI: https://doi.org/10.1038/322557a0
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