Abstract
Thy-1, a glycoprotein of relative molecular mass 25,000 (25K), is a major constituent of the cell surface of mouse thymocytes, peripheral T cells and neurones1. In man, Thy-1 is present on neurones and on a small percentage of thymocytes, but is absent from peripheral T cells2. The amino-acid3,4 and complementary DNA5–7 sequences of Thy-1 indicate that it has a structure similar to an isolated V (variable region) domain of immunoglobulin4. Although the function of Thy-1 is unknown, the ability of different anti-Thy-1 monoclonal antibodies to activate murine T cells8,9 or induce functional changes in neuronal cells in vitro10 suggests that Thy-1 is involved in transmembrane signalling. We now show that crosslinking of murine Thy-1 triggers a rapid rise in the cytoplas-mic free calcium concentration ([Ca2+]i), not only in murine T cells and Thy-1.2-transfected human T cells, but also in murine B-lymphoma cells transfected with the murine thy-1.2 gene. These results indicate that the generation and transduction of the signal leading to the rise in [Ca2+]i is independent of the T-cell receptor and other T-cell-spec i fie molecules. The preservation of the [Ca2+]i-modulating function of Thy-1 in various lymphoid cells of two species further suggests that the necessary signal either originates in the Thy-1 molecule itself or is generated in concert with a highly conserved molecules(s) associated with Thy-1.
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Kroczek, R., Gunter, K., Germain, R. et al. Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes. Nature 322, 181–184 (1986). https://doi.org/10.1038/322181a0
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DOI: https://doi.org/10.1038/322181a0
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