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Expression of a translocated c-abl gene in hybrids of mouse fibroblasts and chronic myelogenous leukaemia cells

Nature volume 319, pages 331–333 (1986)Cite this article

Abstract

Chronic myelogenous leukaemia (CML) is a clonal disease arising from malignant transformation of pluripotent hematopoietic stem cells. In most cases, it is characterized by the presence of the Philadelphia (Ph1) chromosome (22q—) which results from a reciprocal translocation between chromosomes 9 and 22 (refs 1–3). In this translocation, the human homologue of the Abelson virus oncogene, c-abl, normally on chromosome 9, is moved to chromosome 22, while c-sis, the cellular homologue of the simian sarcoma virus oncogene, is moved from chromosome 22 to chromosome 9 (refs 4–6). CML cells carrying the t(9; 22) chromosomal translocation are known to produce an 8-kilobase (kb) c-abl transcript in addition to the normal 6- and 7-kb transcripts6–9 and to express the normal p145 abl protein and a p210 c-abl protein possessing a tyrosine kinase activity not detected in the p145 species10–12. Results of our analyses using somatic cell hybrids between a mouse fibroblast line and two human CML-derived cell lines which carry the Ph1 chromosome and are phenotypically identical to the fibroblast parent indicate that only the hybrid cells containing Ph1 chromosome express both the 8-kb c-abl RNA and the p210 protein. Thus, expression of the altered c-abl transcripts and protein depends on the presence of the Ph1 chromosome and is not myeloid-specific.

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Author notes

  1. Agata Giallongo

    Present address: Istituto di Biologia dello Suiluppo, Via Archirafi 20, 90100, Palermo, Italy

Authors and Affiliations

  1. Wistar Institute of Anatomy and Biology, 36th Street at Spruce, Philadelphia, Pennsylvania, 19104, USA

    Danuta Kozbor, Agata Giallongo, Michael E. Sierzega, Louise C. Showe & Carlo M. Croce

  2. Department of Microbiology and Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California, 90024, USA

    James B. Konopka & Owen N. Witte

Authors
  1. Danuta Kozbor
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  5. Owen N. Witte
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  7. Carlo M. Croce
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Kozbor, D., Giallongo, A., Sierzega, M. et al. Expression of a translocated c-abl gene in hybrids of mouse fibroblasts and chronic myelogenous leukaemia cells. Nature 319, 331–333 (1986). https://doi.org/10.1038/319331a0

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