Abstract
Cauliflower mosaic virus (CaMV) is a double-stranded DNA plant virus1–3 for which evidence has been presented that its replicative cycle involves a reverse transcription step4–14. Until now, however, there has been no direct evidence for such a step. The sequence of the CaMV genome contains a number of open reading frames, one of which (ORF V) encodes a protein of predicted sequence homologous to retroviral reverse transcriptases10. We have cloned this gene, and expressed it in the yeast Saccharomyces cerevisiae. We report here that yeast expressing this gene accumulate significant levels of reverse transcriptase activity. This provides strong evidence that CaMV really does replicate through an RNA intermediate, converted to DNA by reverse transcriptase.
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Takatsuji, H., Hirochika, H., Fukushi, T. et al. Expression of cauliflower mosaic virus reverse transcriptase in yeast. Nature 319, 240–243 (1986). https://doi.org/10.1038/319240a0
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DOI: https://doi.org/10.1038/319240a0
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