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Activated human monocytes express the c-sis proto-oncogene and release a mediator showing PDGF-like activity

Nature volume 319, pages 158–160 (1986)Cite this article

Abstract

Current ideas about the mechanism of wound healing and the pathogenesis of atherosclerosis, pulmonary fibrosis and hepatic fibrosis suggest a central role for the mononuclear phagocyte in attracting and/or stimulating the proliferation of mesenchymal cells1–5. We demonstrate here that activated human blood monocytes, but not resting monocytes, release a mediator that attracts smooth muscle cells and cooperates with other mediators to stimulate fibroblast proliferation. This mediator is very similar to platelet-derived growth factor (PDGF): its Chromatographic properties and chemical stability are similar to those of PDGF6,7, it competes with 125I-PDGF for binding to fibroblasts and it immunoprecipitates with anti-PDGF antibodies. In parallel, stimulated monocytes, but not resting monocytes, express the c-sis proto-oncogene, a gene coding for one of the PDGF chains8,9, consistent with the concept that expression of the c-sis proto-oncogene may be involved in the ability of mononuclear phagocytes to modulate the accumulation of mesenchymal cells.

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References

  1. Leibovich, S. J. & Ross, R. Am. J. Path. 78, 71–100 (1975).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Gerrity, R. G. Am. J. Path. 103, 181–190 (1981).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Faggiotto, A., Ross, R. & Harker, L. Arteriosclerosis 4, 323–340 (1984).

    Article  CAS  PubMed  Google Scholar 

  4. Crystal, R. G., Bitterman, P. B., Rennard, S. I., Hance, A. J. & Keogh, B. A. New Engl. J. Med. 310, 154–166; 235–244 (1984).

    Article  CAS  PubMed  Google Scholar 

  5. Kent, G. et al. Proc. natn. Acad. Sci. U.S.A. 73, 3719–3722 (1976).

    Article  ADS  CAS  Google Scholar 

  6. Westermark, B. et al. in Growth and Maturation Factors Vol.1 (ed. Guroff, G.) 73–115 (Wiley, New York, 1983).

    Google Scholar 

  7. Deuel, T. F. & Huang, J. S. J. clin. Invest. 74, 669–676 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Johnsson, A. et al. EMBO J. 3, 921–928 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Chiu, I. M. et al. Cell 37, 123–129 (1984).

    Article  CAS  PubMed  Google Scholar 

  10. Grotendorst, G. R., Pencev, D., Martin, G. R. & Sodek, J. in Soft and Hard Tissue Repair (eds Hunt, T. K., Heppenstall, R. B., Pines, E. & Rovee, D.) 20–40 (Praeger, New York, 1984).

    Google Scholar 

  11. Pledger, W. J., Stiles, C. D., Antoniades, H. N. & Scher, C. D. Proc. natn. Acad. Sci. U.S.A. 74, 4481–4485 (1977).

    Article  ADS  CAS  Google Scholar 

  12. Bitterman, P. B., Rennard, S. I., Adelberg, S. & Crystal, R. G. J. Cell Biol. 97, 1925–1932 (1983).

    Article  CAS  PubMed  Google Scholar 

  13. Alitalo, K., Hovi, T. & Vaheri, A. J. exp. Med. 151, 602–613 (1980).

    Article  CAS  PubMed  Google Scholar 

  14. Shimokado, K., Raines, E. W., Madtes, D. K. & Ross, R. J. Leukocyte Biol. 37, 742–743 (1985).

    Google Scholar 

  15. Leibovich, S. J. & Ross, R. Am. J. Path. 84, 501–514 (1976).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Firtel, R. A. Cell 24, 6–7 (1981).

    Article  CAS  PubMed  Google Scholar 

  17. Dalla Favera, R., Gelmann, E. P., Gallo, R. C. & Wong-Staal, F. Nature 292, 31–35 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  18. Devare, S. G., Reddy, E. P., Law, J. D., Robbins, K. C. & Aaronson, S. A. Proc. natn. Acad. Sci. U.S.A. 80, 731–735 (1983).

    Article  ADS  CAS  Google Scholar 

  19. Doolittle, R. F. Science 221, 275–277 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Waterfield, M. D. et al. Nature 304, 35–39 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  21. Robbins, K. C., Antoniades, H. N., Devare, S. G., Hunkapiller, M. W. & Aaronson, S. A. Nature 305, 605–608 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  22. Heldin, C. H., Westermark, B. & Wasteson, Å. J. cell. Physiol. 105, 235–246 (1980).

    Article  CAS  PubMed  Google Scholar 

  23. Nistér, M., Heldin, C. H., Wasteson, Å. & Westermark, B. Ann. N.Y. Acad. Sci. 397, 25–33 (1982).

    Article  ADS  PubMed  Google Scholar 

  24. Eva, A. et al. Nature 295, 116–119 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  25. Graves, D. T. et al. Science 226, 972–974 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  26. Barrett, T. B., Gajdusek, C. M., Schwartz, S. M., McDougall, J. K. & Benditt, E. P. Proc. natn. Acad. Sci. U.S.A. 81, 6772–6774 (1984).

    Article  ADS  CAS  Google Scholar 

  27. Jaye, M. et al. Science 228, 882–885 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  28. DiCorleto, P. E. & Bowen-Pope, D. F. Proc. natn. Acad. Sci. U.S.A. 80, 1919–1923 (1983).

    Article  ADS  CAS  Google Scholar 

  29. Nilsson, J., Sjölund, M., Palmberg, L., Thyberg, J. & Heldin, C. H. Proc. natn. Acad. Sci. U.S.A. 82, 4418–4422 (1985).

    Article  ADS  CAS  Google Scholar 

  30. Goustin, A. S. et al. Cell 41, 301–312 (1985).

    Article  CAS  PubMed  Google Scholar 

  31. Shimokado, K. et al. Cell 43, 277–286 (1985).

    Article  CAS  PubMed  Google Scholar 

  32. Bleiberg, I., Harvey, A. K., Smale, G. & Grotendorst, G. R. J. cell. Physiol. 123, 161–166 (1985).

    Article  CAS  PubMed  Google Scholar 

  33. Ross, R. J. Cell Biol. 50, 172–186 (1971).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Antoniades, H. N., Scher, C. D. & Stiles, C. D. Proc. natn. Acad. Sci. U.S.A. 76, 1809–1813 (1979).

    Article  ADS  CAS  Google Scholar 

  35. Deuel, T. F. et al. J. biol. Chem. 256, 8896–8899 (1981).

    CAS  PubMed  Google Scholar 

  36. Heldin, C. H., Westermark, B. & Wasteson, Å. Biochem. J. 193, 907–913 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Raines, E. W. & Ross, R. J. biol. Chem. 257, 5154–5160 (1982).

    CAS  PubMed  Google Scholar 

  38. Bitterman, P. B., Rennard, S. I., Hunninghake, G. W. & Crystal, R. G. J. clin Invest. 70, 806–822 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Hunter, W. M. & Greenwood, F. C. Nature 194, 495–496 (1962).

    Article  ADS  CAS  PubMed  Google Scholar 

  40. Heldin, C. H., Westermark, B. & Wasteson, Å. Proc. natn. Acad. Sci. U.S.A. 78, 3664–3668 (1981).

    Article  ADS  CAS  Google Scholar 

  41. Glisin, V., Crkvenjakov, R. & Byus, C. Biochemistry 13, 2633–2637 (1974).

    Article  CAS  PubMed  Google Scholar 

  42. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  43. Lehrach, H., Diamond, D., Wozney, J. M. & Boedtker, H. Biochemistry 16, 4743–4751 (1977).

    Article  CAS  PubMed  Google Scholar 

  44. Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

    Article  CAS  PubMed  Google Scholar 

  45. Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).

    Article  ADS  CAS  Google Scholar 

  46. Gunning, P. et al. Molec. cell. Biol. 3, 787–795 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Author notes

  1. Peter B. Bitterman

    Present address: University of Minnesota, Minneapolis, Minnesota, 55455, USA

  2. Jean-Francois Mornex

    Present address: Hôpital Louis Pradel, 69394, Cedex 3, Lyon, France

  3. Gary R. Grotendorst

    Present address: Medical University of South Carolina, Charleston, South Carolina, 29425, USA

Authors and Affiliations

  1. Pulmonary Branch, National Heart, Lung, and Blood Institute, and Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Yves Martinet, Peter B. Bitterman, Jean-Francois Mornex, Gary R. Grotendorst, George R. Martin & Ronald G. Crystal

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  4. Gary R. Grotendorst
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  5. George R. Martin
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  6. Ronald G. Crystal
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Martinet, Y., Bitterman, P., Mornex, JF. et al. Activated human monocytes express the c-sis proto-oncogene and release a mediator showing PDGF-like activity. Nature 319, 158–160 (1986). https://doi.org/10.1038/319158a0

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