Abstract
During platelet activation there is a major reorganization in the platelet cytoskeleton that accompanies a rapid change in platelet shape1,2. Many of the events associated with activation are attributed to a rise in calcium concentration within the platelet cytoplasm3,4. One direct consequence of the elevated calcium is the activation of a calcium-dependent protease that cleaves a major platelet protein of relative molecular mass (Mr) ∼235,000 (235K) to 200K (refs 5, 6). This protein, P235, has been purified6 and reported to interact with actin7, but the significance of the proteolytic cleavage is unknown. Talin, a cytoskeletal protein in smooth muscle and fibroblasts8,9, binds vinculin10 and, together with vinculin8,9,11,12, is localized in fibroblasts at sites of actin–membrane attachment. Talin and P235 have similar purification procedures, sedimentation coefficients and Stokes' radii (ref. 6 and Molony et al., unpublished observations). Of particular significance, talin is readily cleaved by proteases from ∼215K to a fragment of ∼190K27. Given these similarities we have investigated the possible relationship between these proteins. Here we demonstrate that platelet P235 is recognized by anti-talin antibody and that it binds vinculin. Both proteins are cleaved in vitro by the calcium-activated protease to yield similar fragments. We conclude that P235 corresponds to the platelet form of talin.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Nachmias, V. T. Semin. Hemat. 20, 261–281 (1983).
Fox, J. E. B. & Phillips, D. R. Semin. Hemat. 20, 243–260 (1983).
Detwiler, T. C., Charo, I. F. & Feinman, R. D. Thromb. Haem. 110, 207–211 (1978).
White, J. G., Rao, F. H. R. & Gerrard, J. M. Am. J. Path. 77, 135–149 (1974).
Fox, J. E. B., Goll, D. E., Reynolds, C. C. & Phillips, D. R. J. biol. Chem. 260, 1060–1066 (1985).
Collier, N. C. & Wang, K. J. biol. Chem. 257, 6937–6943 (1982).
Collier, N. C. & Wang, K. FEBS Lett. 143, 205–210 (1982).
Burridge, K., Kelly, T. & Connell, L. Phil. Trans. R. Soc. B299, 291–299 (1982).
Burridge, K. & Connell, L. J. Cell Biol. 97, 359–367 (1983).
Burridge, K. & Mangeat, P. Nature 308, 744–746 (1984).
Geiger, B. Cell 18, 193–205 (1979).
Burridge, K. & Feramisco, J. R. Cell 19, 587–595 (1980).
Otto, J. J. Cell Biol. 97, 1283–1287 (1983).
Wilkins, J. A., Chen, K. Y. & Lin, S. Biochem. biophys. Res. Commun. 116, 1026–1032 (1983).
Jockusch, B. M. & Isenberg, G. Proc. natn. Acad. Sci. U.S.A. 78, 3005–3009 (1981).
Wilkins, J. A. & Lin, S. Cell 28, 83–90 (1982).
Burridge, K. & Feramisco, J. R. Cold Spring Harb. Symp. quant. Biol. 46, 587–597 (1982).
Evans, R. R., Robson, R. M. & Stromer, M. H. J. biol. Chem. 259, 3916–3925 (1984).
Rosenfeld, G. C., Hou, D. C., Dingus, J., Meza, I. & Bryan, J. J. Cell Biol. 100, 669–676 (1985).
Laemmli, U. K. Nature 227, 680–685 (1970).
Burridge, K., Kelly, T. & Mangeat, P. J. Cell Biol. 95, 478–486 (1982).
Davis, J. & Bennett, V. J. biol. Chem. 258, 7757–7766 (1983).
Towbin, H. et al. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).
Batteiger, B., Newhall, W. J. & Jones, R. B. J. immun. Meth. 55, 297–307 (1982).
Hunter, W. M. & Greenwood, F. C. Nature 194, 495–496 (1962).
Croall, D. E. & DeMartino, G. N. Biochim. biophys. Acta 788, 348–355 (1984).
Beckerle, M. C. et al. J. Cell Biochem (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
O'Halloran, T., Beckerle, M. & Burridge, K. Identification of talin as a major cytoplasmic protein implicated in platelet activation. Nature 317, 449–451 (1985). https://doi.org/10.1038/317449a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/317449a0
This article is cited by
-
Calpain-mediated proteolysis of talin regulates adhesion dynamics
Nature Cell Biology (2004)
-
Decreased adhesion of oxidized LDL-stimulated platelets caused by cytochalasin D
Cell and Tissue Research (1995)
-
Interaction of plasma membrane fibronectin receptor with talin—a transmembrane linkage
Nature (1986)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.