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Induction of proliferation or transformation of neuroretina cells by the mil and myc viral oncogenes

Nature volume 316, pages 559–562 (1985)Cite this article

Abstract

The genome of the avian retro virus MH2 contains, in addition to the v-myc oncogene shared with three other avian retroviruses (MC29, CMII and OK-10), a second cell-derived oncogene, v-mil (refs 1–3). Like the three other viruses, which contain only v-myc, MH2 induces mainly liver and kidney carcinomas in fowl and transforms fibroblasts and macrophages in vitro4. However, MH2 and MC29 differ in their biological properties when assayed on cultures of chicken embryo neuroretina (NR) cells. Indeed, NR cells, which normally do not multiply in vitro, are induced to proliferate and become transformed upon infection with MH2, whereas infection with MC29 has no apparent effect on these cells5,6. To analyse the functions of the two oncogenes of MH2, we isolated spontaneous and in vitro-constructed mutants of this virus and investigated their effects on NR cell multiplication and transformation. We report here that expression of v-mil is sufficient to induce NR cell proliferation, although it does not result in cell transformation. In addition, viruses expressing only the v-myc oncogene fail to induce any detectable change in NR cells. However, cooperation of the two oncogenes is required to achieve transformation of NR cells by MH2.

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References

  1. Coll, J., Righi, M., de Taisne, C., Dissous, C., Gegonne, A. & Stehelin, D. EMBO J. 2, 2189–2194 (1983).

    Article  CAS  Google Scholar 

  2. Jansen, H. W., Ruckert, B., Lurz, R. & Bister, K. EMBO J. 2, 1969–1975 (1983).

    Article  CAS  Google Scholar 

  3. Jansen, H. W., Trachmann, C. & Bister, K. Virology 137, 217–224 (1984).

    Article  CAS  Google Scholar 

  4. Graf, T. & Stehelin, D. Biochim. Biophys. Acta 651, 245–271 (1982).

    CAS  PubMed  Google Scholar 

  5. Calothy, G. et al. Cold Spring Harb. Symp. quant. Biol. 44, 983–990 (1979).

    Article  Google Scholar 

  6. Calothy, G., Poirier, F., Crisanti-Combes, P. & Pessac, B. in Avion Tumor Viruses (eds Barlati, S. & de Giuli-Morgham, C.) 122–133 (Piccin, Padua, (1978).

    Google Scholar 

  7. Crisanti-Combes, P., Privat, A., Pessac, B. & Calothy, G. Cell Tissue Res. 185, 159–173 (1977).

    Article  Google Scholar 

  8. Crisanti-Combes, P. et al. Cell Differentiation 11, 45–54 (1982).

    Article  CAS  Google Scholar 

  9. Hu, S. S. F., Moscovici, C. & Vogt, P. K. Virology 89, 162–178 (1978).

    Article  CAS  Google Scholar 

  10. Ramsay, G. M., Hayman, M. J. & Bister, K. EMBO J. 1, 111–116 (1982).

    Article  Google Scholar 

  11. Saule, S. et al. EMBO J. 2, 805–809 (1983).

    Article  CAS  Google Scholar 

  12. Pachl, C., Biegalke, B. & Linial, M. J. Virol. 45, 133–139 (1983).

    Article  CAS  Google Scholar 

  13. Pachl, C., Schubach, W., Eisenman, R. & Linial, M. Cell 33, 335–344 (1983).

    Article  CAS  Google Scholar 

  14. Galibert, F. Dupont de Dinechin, S., Righi, M. & Stehelin, D. EMBO J. 3, 383–387 (1984).

    Article  Google Scholar 

  15. Pessac, B. & Calothy, G. Science 185, 709–710 (1974).

    Article  ADS  CAS  Google Scholar 

  16. Cohen, S., Fava, R. A. & Sawyer, T. S. Proc. natn. Acad. Sci. U.S.A. 79, 6237–6241 (1982).

    Article  ADS  CAS  Google Scholar 

  17. Heldin, C. H., Ek, B. & Ronnstrand, L. J. biol. Chem. 258, 10054–10061 (1983).

    CAS  PubMed  Google Scholar 

  18. Bunte, T., Greiser-Wilke, J. & Moeling, K. EMBO J. 2, 1087–1092 (1983).

    Article  CAS  Google Scholar 

  19. Moelling, K., Heimann, B., Beimling, P., Rapp, U. & Sander, T. Nature 312, 558–561 (1984).

    Article  ADS  CAS  Google Scholar 

  20. Roussel, M. et al. Nature 281, 452–455 (1979).

    Article  ADS  CAS  Google Scholar 

  21. Kan, N. C. et al. Proc. natn. Acad. Sci. U.S.A. 81, 3000–3004 (1984).

    Article  ADS  CAS  Google Scholar 

  22. Ghysdael, J., Neil, J. C., Wallbank, A. M. & Vogt, P. K. Virology 11, 386–400 (1981).

    Article  Google Scholar 

  23. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  24. Watson, K. D., Reddy, P. E., Duesberg, P. H. & Papas, T. S. Proc. natn. Acbd. Sci. U.S.A. 80, 2146–2150 (1983).

    Article  ADS  CAS  Google Scholar 

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Author notes

  1. C. Bechade and G. Calothy: Institut Curie-Biologic, BAT. 110, 91405 Orsay, France

  2. B. Pessac: INSERM U.178, Hôpital Broussais, 75654 Paris Cedex 14, France

Authors and Affiliations

  1. Unité associeé INSERM-CNRS d'Oncologie Moléculaire, Institut Pasteur, 1 rue Calmette, 59019, Lille, France

    C. Bechade, G. Calothy, B. Pessac, P. Martin, J. Coll, F. Denhez, S. Saule, J. Ghysdael & D. Stéhelin

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  1. C. Bechade
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  8. J. Ghysdael
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  9. D. Stéhelin
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Bechade, C., Calothy, G., Pessac, B. et al. Induction of proliferation or transformation of neuroretina cells by the mil and myc viral oncogenes. Nature 316, 559–562 (1985). https://doi.org/10.1038/316559a0

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