Abstract
SEWA tumour cells are derived from an osteosarcoma induced in an A.SW mouse by infection with polyoma virus1. Cytogenetic analyses have revealed three different characteristic chromosomal abnormalities diagnostic for the presence of amplified genes: ‘double minutes’ (DMs), homogeneously staining chromosomal regions (HSRs) and C-bandless chromosomes (CMs; for review see ref. 2). DMs may undergo fluctuation in number depending on the conditions in which the cells grow. Their number usually increases after injection of cells into a mouse and often is reduced to undetectable levels when the cells are explanted back into tissue culture; when the cells are re-introduced into the mouse, they again acquire multiple DMs3. We show here that cells of SEWA lines carrying DMs, HSRs or CMs contain amplified copies of the proto-oncogene c-myc and enhanced levels of c-myc messenger RNA and c-myc protein. DMs or CMs are the sites of c-myc amplification in two different SEWA lines.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Sjogren, H. O., Hellstrom, I. & Klein, G. Cancer Res. 21, 329–337 (1961).
Levan, A., Levan, G. & Mandahl, N. in Genes, Chromosomes and Neoplasia (eds Arrighi, F. E., Rao, P. N. & Stubblefield, E.) 223–251 (Raven, New York, 1981).
Levan, G., Mandahl, N., Bengtson, B. P. & Levan, A. Hereditas 86, 75–90 (1977).
Schwab, M., Alitalo, K., Varmus, H. E., Bishop, J. M. & George, D. Nature 303, 497–501 (1983).
Alitalo, K., Schwab, M., Lin, C. C., Varmus, H. E. & Bishop, J. M. Proc. natn. Acad. Sci. U.S.A. 80, 1707–1711 (1983).
Ramsay, G., Evan, G. & Bishop, J. M. Proc. natn. Acad. Sci. U.S.A. 81, 7742–7746 (1984).
Schwab, M., Alitalo, K., Varmus, H. E. & Bishop, J. M. in The Cancer Cell Vol. 2 (eds VandeWoude, G. F., Levine, A. J., Topp, W. C. & Watson, J. D.), 215–220 (Cold Spring Harbor Laboratory, New York, 1984).
Levan, G., Hauser-Urfer, I., Pero, R., Mitelman, F. & Levan, A. 7th int. Chromosome Conf., 96–97 (Oxford University Press, 1980).
Blumenthal, A., Dieden, J., Lapp, L. & Sedat, J. W. J. Cell Biol. 81, 255–259 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schwab, M., Ramsay, G., Alitalo, K. et al. Amplification and enhanced expression of the c-myc oncogene in mouse SEWA tumour cells. Nature 315, 345–347 (1985). https://doi.org/10.1038/315345a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/315345a0
This article is cited by
-
Amplification and overexpression of the hepatocyte growth factor receptor (HGFR/MET) in rat DMBA sarcomas
Oncogene (1999)
-
Selection of cells with different chromosomal localizations of the amplified c-myc gene during in vivo and in vitro growth of the breast carcinoma cell line SW 613-S
Chromosoma (1989)
-
Control of myogenic differentiation by cellular oncogenes
Molecular Neurobiology (1988)
-
Localization of viral transforming sequences within marker chromosomes associated with tumor formation and progression in a murine fibrosarcoma
Cytotechnology (1987)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.