Abstract
Serological and molecular genetic analyses of T-cell clones have shown that the T-cell antigen receptor1 apparently comprises two glycosylated, disulphide-linked polypeptide chains (α and β), both of which span the cell membrane. Cloning of the genes encoding the two chains from mouse and human DNA has shown that the α- and β-chains are composed of variable (V) and conserved (C) regions in agreement with peptide mapping data2–11. Gene segments encoding variable and conserved domains of the β-chain have been identified and undergo rearrangements during T-cell differentiation. The genes encoding the α-chain, so far described at the level of complementary DNA clones, also identify DNA rearrangements. Thus, the genes encoding the T-cell receptor show the same structure and dynamic behaviour as immunoglobulin genes, indicating that the two gene families belong to the same supergene family5,12,13; this evolutionary relationship is supported by the fact that the genes encoding the β-chain of the T-cell receptor are closely linked to immunoglobulin κ light-chain genes on chromosome 6 in mouse14. In man, however, the β genes map to chromosome 7 (ref. 14) whereas the κ-chain genes are located on chromosome 2, indicating that linkage between the two gene families is not needed for proper expression. Here we describe genomic clones encoding the constant portion of the T-cell receptor α-chain and map the gene to chromosome 14 in mouse, close to the gene for purine nucleoside phosphorylase (Np-2) which, in man, has been associated with T-cell immunodeficiencies.
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DembiĆ, Z., Bannwarth, W., Taylor, B. et al. The gene encoding the T-cell receptor α-chain maps close to the Np-2 locus on mouse chromosome 14. Nature 314, 271–273 (1985). https://doi.org/10.1038/314271a0
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DOI: https://doi.org/10.1038/314271a0
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