Abstract
U2 RNA is one of the abundant, highly conserved species of small nuclear RNA (snRNA) molecules implicated in RNA processing1. As is typical of mammalian snRNAs, human U1 and U2 are each encoded by a multigene family. In the human genome, defective copies of the genes (pseudogenes) far outnumber the authentic genes2. The majority or all of the 35 to 100 bona fide U1 genes3–5 have at least 20 kilobases (kb) of nearly perfect 5′ and 3′ flanking homology in common with each other4,6; these U1 genes are clustered loosely in chromosome band 1p36 (refs 5, 7) with intergenic distances exceeding 44 kb. In contrast, the 10 to 20 U2 genes are clustered tightly in a virtually perfect tandem array which has a strict 6-kb repeating unit8,9. We report here the assignment, by in situ hybridization, of the U2 gene cluster to chromosome 17, bands q21–q22. Surprisingly, this region is one of three major adenovirus 12 modification sites which undergo chromosome decondensation (‘uncoiling’) in permissive human cells infected by highly oncogenic strains of adenovirus10,11. The two other major modification sites, 1p36 and 1q21, coincide with the locations of U1 genes5,7 and class I U1 pseudogenes12, respectively. We suggest that snRNA genes are the major targets of viral chromosome modification.
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Lindgren, V., Ares, M., Weiner, A. et al. Human genes for U2 small nuclear RNA map to a major adenovirus 12 modification site on chromosome 17. Nature 314, 115–116 (1985). https://doi.org/10.1038/314115a0
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DOI: https://doi.org/10.1038/314115a0
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