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Characterization of a steroid hormone receptor gene and mRNA in wild-type and mutant cells

Nature volume 312, pages 779–781 (1984)Cite this article

Abstract

The effects of steroid hormones are mediated by intracellular hormone-specific receptor proteins; the interaction between the hormone and its receptor increases the affinity of the receptor for nuclear binding sites, thereby modulating the expression of specific genes1. The glucocorticoid receptor is a soluble protein of relative molecular mass (Mr) 94,000 (94K), present at a low relative abundance (≤0.01%); it has been purified to near-homogeneity2,3, and specific antisera and monoclonal antibodies have been produced4–6. Purified glucocorticoid receptor binds in vitro with high affinity to defined regions of DNA near regulated promoters7–10, and sequences essential for these interactions are functional in vivo as hormone-dependent transcriptional enhancer elements11,12. We have now cloned complementary DNA (cDNA) for the rat liver glucocorticoid receptor and we describe here a 2.6-kilobase (kb) receptor cDNA isolated following polysome immuno-enrichment of receptor messenger RNA with glucocorticoid receptor-specific antibodies. The receptor appears to be encoded by a single-copy gene which specifies a ∼6-kb transcript in rat and mouse cells; this mRNA is altered quantitatively and qualitatively in several mutant cell lines with specific defects in receptor function.

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Author notes

  1. Sam Okret, Ann-Charlotte Wikström, Örjan Wrange and Jan-Åke Gustafsson: Department of Medical Nutrition, Karolinska Institutet, Huddinge Hospital F69, S-14186 Huddinge, Sweden

Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, 94143, USA

    Roger Miesfeld, Sam Okret, Ann-Charlotte Wikström, Örjan Wrange, Jan-Åke Gustafsson & Keith R. Yamamoto

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  1. Roger Miesfeld
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  2. Sam Okret
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  5. Jan-Åke Gustafsson
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  6. Keith R. Yamamoto
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Miesfeld, R., Okret, S., Wikström, AC. et al. Characterization of a steroid hormone receptor gene and mRNA in wild-type and mutant cells. Nature 312, 779–781 (1984). https://doi.org/10.1038/312779a0

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