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The structure, rearrangement and expression of Dβ gene segments of the murine T-cell antigen receptor

Nature volume 311, pages 344–349 (1984)Cite this article

Abstract

It has been postulated that the variable region of the β-polypeptide of the murine T-cell antigen receptor is encoded by three distinct germ-line gene segments—variable (Vβ), diversity (Dβ) and joining (Jβ)—that are rearranged to generate a Vβ gene. Germ-line Vβ and Jβ gene segments have been isolated previously. Here we report the isolation and characterization of two germ-line Dβ gene segments that have recognition signals for DNA rearrangement strikingly similar to those found in the three immunoglobulin gene families and in Vβ and Jβ gene segments. The Dβ and Jβ segments can join in the absence of Vβ gene segment rearrangement and these rearranged sequences are transcribed in some T cells.

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References

  1. Early, P. & Hood, L. in Genetic Engineering (eds Setlow, J. K. & Hollaender, A.) 157–188 (Plenum, New York, 1981).

    Book  Google Scholar 

  2. Tonegawa, S. Nature 302, 575–581 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Honjo, T. A. Rev. Immun. 1, 499–528 (1983).

    Article  CAS  Google Scholar 

  4. Brack, C., Hirama, M., Lenhard-Schuller, R. & Tonegawa, S. Cell 15, 1–14 (1978).

    Article  CAS  PubMed  Google Scholar 

  5. Seidman, J., Max, E. & Leder, P. Nature 280, 370–375 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  6. Sakano, H., Huppi, K., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Early, P., Huang, H., Davis, M., Calame, K. & Hood, L. Cell 19, 981–992 (1980).

    Article  CAS  PubMed  Google Scholar 

  8. Sakano, H., Maki, R., Kurosawa, Y., Roeder, W. & Tonegawa, S. Nature 286, 676–683 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Schilling, J., Clevinger, B., Davie, J. M. & Hood, L. Nature 283, 35–40 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Weigert, M., Perry, R., Kelley, D., Hunkapiller, T., Schilling, J. & Hood, L. Nature 283, 497–499 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  11. Sakano, H., Kurosawa, Y., Weigert, M. & Tonegawa, S. Nature 290, 562–565 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Kurosawa, Y. et al. Nature 290, 565–570 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  13. Alt, F. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 79, 4118–4122 (1982).

    Article  ADS  CAS  Google Scholar 

  14. Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).

    Article  ADS  CAS  Google Scholar 

  15. Allison, J. P., McIntyre, B. W. & Bloch, D. J. Immun. 129, 2293–2300 (1982).

    CAS  PubMed  Google Scholar 

  16. Haskins, K. et al. J. exp. Med. 157, 1149–1169 (1983).

    Article  CAS  PubMed  Google Scholar 

  17. Meuer, S. C. et al. J. exp. Med. 157, 705–719 (1983).

    Article  CAS  PubMed  Google Scholar 

  18. Hedrick, S. M., Cohen, D. I., Nielsen, E. A. & Davis, M. M. Nature 308, 149–153 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  19. Hedrick, S. M., Nielsen, E. A., Kavaler, J., Cohen, D. I. & Davis, M. M. Nature 308, 153–158 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Yanagi, Y. et al. Nature 308, 145–153 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  21. Chien, Y., Gascoigne, N., Kavaler, J., Lee, N. & Davis, M. Nature 309, 322–326 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  22. Siu, G. et al. Cell 37, 393–401 (1984).

    Article  CAS  PubMed  Google Scholar 

  23. Malissen, M. et al. Cell 37, 1101–1110 (1984).

    Article  CAS  PubMed  Google Scholar 

  24. Horvath, J. biol. Chem. (submitted).

  25. Kurosawa, Y. & Tonegawa, S. J. exp. Med. 155, 201 (1982).

    Article  CAS  PubMed  Google Scholar 

  26. Kaartinen, M., Griffiths, G., Markham, N. & Milstein, C. Nature 304, 320–324 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  27. Alt, F. W., Rosenberg, N., Enea, V., Siden, E. & Baltimore, D. Molec. cell. Biol. 2, 386–400 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Clark, S. et al. Nature 311, 387–389 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  29. Alt, F., Rosenberg, N., Lewis, S., Thomas, E. & Baltimore, D. Cell 27, 381–390 (1981).

    Article  CAS  PubMed  Google Scholar 

  30. Nelson, K., Haimovich, J. & Perry, R. Molec. cell. biol. 3, 1317–1332 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Early, P., Nottenburg, C., Weissman, I. & Hood, L. Molec. cell. Biol. 2, 829–836 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Reth, M. & Alt, F. Nature (in the press).

  33. Kataoka, T., Niakido, T., Miyata, T., Moriwaki, K. & Honjo, T. J. biol. Chem. 257, 277–285 (1982).

    CAS  PubMed  Google Scholar 

  34. Clarke, C. et al. Nucleic Acids Res. 10, 7731–7749 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Rice, D. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 79, 7862–7865 (1982).

    Article  ADS  CAS  Google Scholar 

  36. Oi, V., Morrison, S., Herzenberg, L. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 80, 825–829 (1983).

    Article  ADS  CAS  Google Scholar 

  37. Gillies, S., Morrison, S., Oi, V. & Tonegawa, S. Cell 33, 717–728 (1983).

    Article  CAS  PubMed  Google Scholar 

  38. Queen, C. & Baltimore, D. Cell 33, 741–748 (1983).

    Article  CAS  PubMed  Google Scholar 

  39. Banerji, J., Olson, L. & Schaffner, W. Cell 33, 729–740 (1983).

    Article  CAS  PubMed  Google Scholar 

  40. Perry, R. P. et al. Proc. natn. Acad. Sci. U.S.A. 77, 1937–1941 (1980).

    Article  ADS  CAS  Google Scholar 

  41. Kemp, D. J., Harris, A. W. & Adams, J. M. Proc. natn. Acad. Sci. U.S.A. 77, 7400–7404 (1980).

    Article  ADS  CAS  Google Scholar 

  42. Walker, I. D. & Harris, A. W. Nature 288, 290–293 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  43. Silhavy, T. S., Benson, S. A. & Emr, S. D. Microbiol. Rev. 47, 313–344 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  44. Alt, F. W., Enea, V., Bothwell, A. L. M. & Baltimore, D. Cell 21, 1–12 (1980).

    Article  CAS  PubMed  Google Scholar 

  45. Hood, L. E., Hunkapiller, T. & Kraig, E. in Modern Cell Biology (ed. McIntosh, J. R.) 305–328 (Liss, New York, 1983).

    Google Scholar 

  46. Williams, A. F. Nature 308, 108–109 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  47. Messing, J. & Vieira, J. Gene 19, 269–276 (1982).

    Article  CAS  PubMed  Google Scholar 

  48. Sanger, F., Coulson, A. R., Barrell, B. G., Smith, A. J. H. & Roe, B. A. J. molec. Biol. 143, 161–178 (1980).

    Article  CAS  PubMed  Google Scholar 

  49. Crews, S., Griffin, J., Huang, H., Calame, K. & Hood, L. Cell 25, 59–66 (1981).

    Article  CAS  PubMed  Google Scholar 

  50. Ollo, R., Auffray, C., Sikorav, J.-L. & Rougeon, F. Nucleic Acids Res. 9, 4099–4108 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Givol, D., Zakut, R., Effron, K., Rechavi, G., Ram, D. & Cohen, J. B. Nature 292, 426–430 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  52. Bothwell, A. L. M., Paskind, M., Reth, M., Imanishi-Kari, T., Rajewsky, K. & Baltimore, D. Cell 24, 625–637 (1981).

    Article  CAS  PubMed  Google Scholar 

  53. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  PubMed  Google Scholar 

  54. Caccia, N. et al. Cell 37, 1091–1099 (1984).

    Article  CAS  PubMed  Google Scholar 

  55. Steinmetz, M. et al. Nature 300, 35–42 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  56. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979).

    Article  CAS  PubMed  Google Scholar 

  57. Kronenberg, M., Davis, M. M., Early, P. W., Hood, L. E. & Watson, J. D. J. exp. Med. 152, 1745–1761 (1980).

    Article  CAS  PubMed  Google Scholar 

  58. Kavaler, J., Davis, M. M. & Chien, Y. Nature 310, 421–424 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Division of Biology, California Institute of Technology, Pasadena, California, 91125, USA

    Gerald Siu, Mitchell Kronenberg, Erich Strauss & Leroy Hood

  2. Department of Pathology, College of Physicians and Surgeons of Columbia University, New York, 10032, USA

    Regina Haars

  3. Department of Medical Biophysics, University of Toronto, Ontario Cancer Institute, Toronto, Canada, M4X 1K9

    Tak W. Mak

Authors
  1. Gerald Siu
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  2. Mitchell Kronenberg
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  3. Erich Strauss
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  4. Regina Haars
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  5. Tak W. Mak
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  6. Leroy Hood
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Siu, G., Kronenberg, M., Strauss, E. et al. The structure, rearrangement and expression of Dβ gene segments of the murine T-cell antigen receptor. Nature 311, 344–349 (1984). https://doi.org/10.1038/311344a0

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