Abstract
Polyoma virus is a papovavirus that productively infects mouse cells. In cells of other species, such as rat cells, polyoma virus is virtually unable to replicate, and a small proportion of infected cells become stably transformed. The ability of polyoma virus to transform infected cells is determined by genes that encode the large, middle and small T antigens1–3 and which are found in the early region of the virus genome. We have inserted the transforming region of polyoma virus into a murine leukaemia virus (MLV) vector, to generate a replication-defective transforming retrovirus which for the first time allows efficient transformation of mouse cells by the polyoma virus middle T gene. During the life cycle of this recombinant virus the intervening sequence present in the original polyoma virus middle T gene was removed. The recombinant virus that we have constructed is analogous to other acutely transforming retroviruses, and demonstrates that the polyoma middle T gene is a dominant transforming oncogene.
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References
Friedmann, T., Esty, A., Laporte, P. & Deininger, P. Cell 17, 715–724 (1979).
Soeda, E., Arrand, J. R., Smolar, N., Walsh, J. E. & Griffin, B. E. Nature 283, 445–453 (1980).
Treisman, R., Cowie, E., Favaloro, J., Jat, P. & Kamen, R. Molec. appl. Genet. 1, 83–92 (1981).
Ito, Y., Brocklehurst, J. R. & Dilbecco, R. Proc. natn. Acad. Sci. U.S.A. 74, 4666–4670 (1977).
Smart, J. E. & Ito, Y. Cell 15, 1426–1437 (1978).
Hutchinson, M. A., Hunter, T. & Eckhart, W. Cell 15, 65–77 (1978).
Eckhart, W., Hutchinson, M. A. & Hunter, T. Cell 18, 925–933 (1979).
Walter, G., Hutchinson, M. A., Hunter, T. & Eckhart, W. Proc. natn. Acad. Sci. U.S.A. 79, 4025–4029 (1982).
Courtneidge, S. A. & Smith, A. E. Nature 303, 534–439 (1983).
Treismann, R., Novak, U., Favaloro, J. & Kamen, R. Nature 292, 595–600 (1981).
Templeton, D. & Eckhart, W. J. Virol. 41, 1014–1024 (1982).
Carmichael, G. G., Schaffhausen, B. S., Dorsky, D. I., Oliver, D. B. & Benjamin, T. L. Proc. natn. Acad. Sci. U.S.A. 79, 3579–3583 (1982).
Rassoulzadegan, M. et al. Nature 300, 713–718 (1982).
Hoffman, J. W. et al. J. Virol. 44, 144–157 (1982).
Graham, F. L. & van der Eb, A. J. Virology 52, 456–467 (1973).
Shimotohno, K. & Temin, H. M. Nature 299, 265–268 (1982).
Laskey, R. A. & Mills, A. D. Eur. J. Biochem. 56, 335–341 (1975).
Shinnick, T. M., Lerner, R. A. & Sutcliffe, J. G. Nature 293, 543–548 (1981).
Rowe, W. P., Pugh, W. E. & Hartley, J. W. Virology 42, 1136–1139 (1970).
Papkoff, J., Hunter, T. & Beemon, K. Virology 101, 91–103 (1980).
Deininger, P. L., Esty, A., LaPorte, P., Hsu, H. & Friedmann, T. Nucleic Acids Res. 8, 855–860 (1980).
Donoghue, D. J. & Hunter, T. Nucleic. Acids Res. 10, 2549–2564 (1982).
Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
Bina-Stein, M., Thoren, M., Salzman, N. & Thompson, J. A. Proc. natn. Acad. Sci. U.S.A. 76, 731–735 (1979).
McClure, D.B., Hightower, M.J. & Topp, W.C. in Growth of Cells in Hormonally Defined Media (eds Sato, H. T., Pardee, A. B. & Sirbasku, D. 345–364 (Cold Spring Harbor, New York, 1982).
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Donoghue, D., Anderson, C., Hunter, T. et al. Transmission of the polyoma virus middle T gene as the oncogene of a murine retrovirus. Nature 308, 748–750 (1984). https://doi.org/10.1038/308748a0
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DOI: https://doi.org/10.1038/308748a0
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