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Preferential integration of yeast transposable element Ty into a promoter region

Nature volume 307, pages 386–388 (1984)Cite this article

Abstract

Mobile genetic elements have been identified in several eukaryotic organisms and some classes have been found to share common structural features with the proviral forms of animal retroviruses1–7. The representatives of this class of mobile elements in the yeast Saccharomyces cerevisiae are called Ty elements8, which could be a useful model system for studying the transposition of retrovirus-like elements. Here we have attempted to answer two questions often raised in discussions of the biological importance of transposition: what is the frequency of spontaneous Ty transposition, and are there certain chromosomal regions into which Ty elements preferentially integrate? We chose the LYS2 gene to investigate these questions because it allows direct selection of both mutants and revertants9. We have found that 2% of spontaneous lys2 mutants are caused by Ty transposition with a preferential integration into the transcription initiation region.

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Author notes

  1. Hermann Eibel

    Present address: Institut für Biologie III, Universität Freiburg, Schänzlestrasse 1, D-7800, Freiburg i.B., FRG

Authors and Affiliations

  1. Microbiology Department, Biozentrum, University of Basel, Klingelbergstrasse 70, Basel, CH-4056, Switzerland

    Hermann Eibel & Peter Philippsen

Authors
  1. Hermann Eibel
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  2. Peter Philippsen
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Eibel, H., Philippsen, P. Preferential integration of yeast transposable element Ty into a promoter region. Nature 307, 386–388 (1984). https://doi.org/10.1038/307386a0

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