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Association of platelet-derived growth factor-induced protein with nuclear material

Nature volume 306, pages 272–274 (1983)Cite this article

Abstract

Platelet-derived growth factor (PDGF) has been proposed to initiate the cell-cycle traverse of density-arrested BALB/c-3T3 cells by rendering quiescent cells ‘competent’ to respond to ‘progression’ factors contained in platelet-poor plasma (PPP)1. PDGF-treated cells remain competent for many hours following PDGF removal; subsequent addition of PPP triggers G0–G1 traversal and entry into S phase1,2. Numerous observations suggest that the competent state reflects the existence of a stable PDGF-induced ‘second signal’ as opposed to a persistent association of PDGF with cells1–3. Several unique proteins have been shown to be synthesized in response to PDGF; of these, the dose-dependent production of ‘pI’ (molecular weight 29,000) was found to correlate closely with the induction of competence4. We report here the further characterization of pI in terms of its time-dependent synthesis, intracellular location and stability. Electrophoretic analysis of nuclear and non-nuclear extracts of PDGF-treated BALB/C-3T3 cells demonstrated that pI is synthesized during the first 6 h of G0–G1 is associated with nuclear material and is stable for 9–12 h. These findings are consistent with the proposed role of pI as the cellular mediator of PDGF.

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References

  1. Pledger, W. J., Stiles, C. D., Antoniades, H. N. & Scher, C. D. Proc. natn. Acad. Sci. U.S.A. 74, 4481–4485 (1977).

    Article  ADS  CAS  Google Scholar 

  2. Singh, J. P., Chaikin, M. A., Pledger, W. J., Scher, C. D. & Stiles, C. D. J. Cell Biol. 96, 1499–1502 (1983).

    Article  Google Scholar 

  3. Smith, J. C. & Stiles, C. D. Proc. natn. Acad. Sci. U.S.A. 78, 4363–4367 (1981).

    Article  ADS  CAS  Google Scholar 

  4. Pledger, W. J., Hart, C. A., Locatell, K. L. & Scher, C. D. Proc. natn. Acad. Sci. U.S.A. 78, 4358–4362 (1981).

    Article  ADS  CAS  Google Scholar 

  5. Pledger, W. J., Howe, P. H. & Leof, E. B. Ann. N.Y. Acad. Sci. 397, 1–10 (1982).

    Article  ADS  CAS  Google Scholar 

  6. Kletzein, R. F. Biochem. J. 196, 853–859 (1981).

    Article  Google Scholar 

  7. Heldin, C. H., Wasteson, A. & Westermark, B. J. biol. Chem. 257, 4216–4221 (1981).

    Google Scholar 

  8. Scher, C. D., Stone, M. E. & Stiles, C. D. Nature 281, 1065–1072 (1979).

    Article  Google Scholar 

  9. Smith, G. J. Analyt. Biochem. 111, 97–104 (1981).

    Article  CAS  Google Scholar 

  10. Dippold, W. G., Jay, G., Deleo, A. B., Khoury, G. & Old, L. J. Proc. natn. Acad. Sci. U.S.A. 78, 1695–1699 (1981).

    Article  ADS  CAS  Google Scholar 

  11. Jay, G. et al. Cold Spring Harb. Symp. quant. Biol. 44, 659–664 (1980).

    Article  CAS  Google Scholar 

  12. Mercer, W. E., Nelson, D., Deleo, A. B., Old, L. J. & Baserga, R. Proc. natn. Acad. Sci. U.S.A. 79, 6309–6312 (1982).

    Article  ADS  CAS  Google Scholar 

  13. Azizkhan, J. C. & Klagsburn, M. Proc. natn. Acad. Sci. U.S.A. 77, 2762–2766 (1980).

    Article  ADS  CAS  Google Scholar 

  14. Sen, A. & Todaro, G. J. Proc. natn. Acad. Sci. U.S.A. 75, 1647–1651 (1981).

    Article  ADS  Google Scholar 

  15. Luka, J., Jornvall, H. & Klein, G. J. Virol. 35, 592–602 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Antoniades, H. N. Proc. natn. Acad. Sci. U.S.A. 78, 7314–7317 (1981).

    Article  ADS  CAS  Google Scholar 

  17. Heldin, C.-H., Westermark, B. & Wasteson, A. Biochem. J. 193, 907–913 (1981).

    Article  CAS  Google Scholar 

  18. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  19. Bonner, W. J. & Laskey, R. A. Eur. J. Biochem. 46, 1279–1283 (1974).

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology and Cancer Research Center, University of North Carolina, Chapel Hill, North Carolina, 27514, USA

    Nancy E. Olashaw & W. J. Pledger

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  1. Nancy E. Olashaw
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  2. W. J. Pledger
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Olashaw, N., Pledger, W. Association of platelet-derived growth factor-induced protein with nuclear material. Nature 306, 272–274 (1983). https://doi.org/10.1038/306272a0

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