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The new yeast genetics

Abstract

Gene cloning and yeast DNA transformation techniques have greatly enhanced the power of classical yeast genetics. It is now possible to isolate any classically defined gene, to alter the yeast genome at will by replacing normal chromosomal sequences with mutated derivatives produced in vitro, and to create DNA molecules that behave as autonomous replicons or minichromosomes. These unique features of the new yeast genetics have been used to study many problems in eukaryotic molecular biology.

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References

  1. Struhl, K., Cameron, J. R. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 73, 1471–1475 (1976).

    Article  ADS  CAS  Google Scholar 

  2. Ratzkin, B. & Carbon, J. Proc. natn. Acad. Sci. U.S.A. 74, 487–491 (1977).

    Article  ADS  CAS  Google Scholar 

  3. Struhl, K. & Davis, R. S. Proc. natn. Acad. Sci. U.S.A. 74, 5255–5259 (1977).

    Article  ADS  CAS  Google Scholar 

  4. Hinnen, A., Hicks, J. B. & Fink, G. R. Proc. natn. Acad. Sci. U.S.A. 75, 1929–1933 (1978).

    Article  ADS  CAS  Google Scholar 

  5. Mortimer, R. K. & Hawthorne, D. C. in The Yeasts Vol. 1 (eds Rose, A. H. & Harrison, J. S.) 386–460 (Academic, New York, 1970).

    Google Scholar 

  6. Hartwell, L. H. Bact. Rev. 38, 164–198 (1974).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Hicks, J. B., Strathern, J. & Herskowitz, I. in DNA Insertions, Plasmids, and Episomes (eds Bukhari, A., Shapiro, J. & Adhya, S. ) 457–462 (Cold Spring Harbor Laboratory, New York, 1977).

    Google Scholar 

  8. Mortimer, R. K. & Schild, D. Microbiol. Rev. 44, 519–571 (1980).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Petes, T. D. A. Rev. Biochem. 49, 845–876 (1980).

    Article  CAS  Google Scholar 

  10. Strathern, J., Jones, E. & Broach, J. (eds) The Molecular Biology of the Yeast Saccharomyces (Cold Spring Harbor Laboratory, New York, 1981).

  11. Roeder, G. S. & Fink, G. R. in Mobile Genetic Elements (ed. Shapiro, J. ) (Academic, New York, in the press).

  12. Nasmyth, K. A. Rev. Genet. (in the press).

  13. Beggs, J. Nature 275, 104–109 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  14. Struhl, K., Stinchcomb, D. T., Scherer, S. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 76, 1035–1039 (1979).

    Article  ADS  CAS  Google Scholar 

  15. Clarke, L. & Carbon, J. Nature 287, 504–509 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  16. Szostak, J. & Blackburn, E. Cell 29, 245–255 (1982).

    Article  CAS  PubMed  Google Scholar 

  17. Hicks, J. B., Hinnen, A. & Fink, G. R. Cold Spring Harb. Symp. quant. Biol. 43, 1305–1313 (1978).

    Article  Google Scholar 

  18. Hsiao, C. L. & Carbon, J. Proc. natn. Acad. Sci. U.S.A. 76, 3829–3833 (1979).

    Article  ADS  CAS  Google Scholar 

  19. Stinchcomb, D. T., Struhl, K. & Davis, R. W. Nature 282, 39–43 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Stinchcomb, D. T., Mann, C. & Davis, R. W. J. molec. Biol. 158, 157–180 (1982).

    Article  CAS  PubMed  Google Scholar 

  21. llth International Conference on Yeast Genetics and Molecular Biology, Montpellier (1982).

  22. Broach, J. R., Strathern, J. N. & Hicks, J. B. Gene 8, 121–133 (1979).

    Article  CAS  PubMed  Google Scholar 

  23. Hicks, J., Strathern, J. N. & Klar, A. J. S. Nature 282, 478–483 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  24. Hinnen, A., Farabaugh, P., Ilgen, C. & Fink, G. R. ICN-UCLA Symp. 14, 43–50 (1979).

    CAS  Google Scholar 

  25. Williamson, V. M., Bennetzen, T., Young, E. T., Nasmyth, K. A. & Hall, B. D. Nature 283, 214–216 (1979).

    Article  ADS  Google Scholar 

  26. Clarke, L. & Carbon, J. Proc. natn. Acad. Sci. U.S.A. 77, 2173–2177 (1980).

    Article  ADS  CAS  Google Scholar 

  27. Nasmyth, K. A. & Reed, S. I. Proc. natn. Acad. Sci. U.S.A. 77, 2119–2123 (1980).

    Article  ADS  CAS  Google Scholar 

  28. Kurjan, J. & Herskowitz, I. Cell 30, 933–943 (1982).

    Article  CAS  PubMed  Google Scholar 

  29. Morse, M. L., Lederberg, E. M. & Lederberg, J. Genetics 41, 758–775 (1956).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Scherer, S. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 76, 4945–4955 (1979).

    Article  ADS  Google Scholar 

  31. Rykowski, M. C., Wallis, J. W., Chow, J. & Grunstein, M. Cell 25, 477–487 (1981).

    Article  CAS  PubMed  Google Scholar 

  32. Neff, N. F., Thomas, J. H., Grisafi, P. G. & Botstein, D. Cell 33, 211–219 (1983).

    Article  CAS  PubMed  Google Scholar 

  33. Shortle, D., Haber, J. & Botstein, D. Science 217, 371–373 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  34. Struhl, K. Gene (in the press).

  35. Struhl, K. Proc. natn. Acad. Sci. U.S.A. 78, 4461–4465 (1981).

    Article  ADS  CAS  Google Scholar 

  36. Struhl, K. Nature 300, 284–288 (1982).

    Article  ADS  CAS  Google Scholar 

  37. Donahue, T. F., Daves, R. S., Lucchini, G. & Fink, G. R. Cell 32, 89–98 (1983).

    Article  CAS  PubMed  Google Scholar 

  38. Faye, G., Leang, D., Tatchell, K., Hall, B. D. & Smith, M. Proc. natn. Acad. Sci. U.S.A. 78, 2258–2262 (1981).

    Article  ADS  CAS  Google Scholar 

  39. Guarente, L. G. & Mason, T. Cell 32, 1279–1286 (1983).

    Article  CAS  PubMed  Google Scholar 

  40. Beier, D. & Young, E. T. Nature 300, 724–727 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  41. Abraham, J. et al. Cold Spring Harb. Symp. quant. Biol. 47, 989–998 (1982).

    Google Scholar 

  42. Struhl, K. & Davis, R. W. J. molec. Biol. 152, 553–568 (1981).

    Article  CAS  PubMed  Google Scholar 

  43. Jacob, F., Ullman, U. & Monod, J. J. molec. Biol. 13, 704–711 (1965).

    Article  CAS  Google Scholar 

  44. Guarente, L. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. 78, 2199–2203 (1981).

    Article  ADS  CAS  Google Scholar 

  45. Rose, M., Casadaban, M. & Botstein, D. Proc. natn. Acad. Sci. U.S.A. 78, 2460–2464 (1981).

    Article  ADS  CAS  Google Scholar 

  46. McNeil, J. B. & Friesen, J. D. Molec. gen. Genet. 184, 386–393 (1981).

    Article  CAS  PubMed  Google Scholar 

  47. Struhl, K. Proc. natn. Acad. Sci. U.S.A. 79, 7385–7389 (1982).

    Article  ADS  CAS  Google Scholar 

  48. Rosenberg, M. & Court, D. A. Rev. Genet. 13, 319–353 (1979).

    Article  CAS  Google Scholar 

  49. Siebenlist, U., Simpson, R. B. & Gilbert, W. Cell 20, 269–281 (1980).

    Article  CAS  PubMed  Google Scholar 

  50. Banerji, J., Rusconi, S. & Schaffner, W. Cell 27, 299–308 (1981).

    Article  CAS  PubMed  Google Scholar 

  51. Moreau, P. et al. Nucleic Acids Res. 9, 6047–6069 (1981).

    Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

  52. Fromm, M. & Berg, P. J. molec. appl. Genet. 1, 457–481 (1982).

    CAS  Google Scholar 

  53. Struhl, K. Cold Spring Harb. Symp. quant. Biol. 47, 901–910 (1982).

    Google Scholar 

  54. Zalkin, H. & Yanofsky, C. J. biol. Chem. 257, 1491–1500 (1982).

    Article  CAS  PubMed  Google Scholar 

  55. Hinnebush, A. G. & Fink, G. R. J. biol. Chem. 258, 5238–5247 (1983).

    Article  Google Scholar 

  56. Guarente, L., Yocum, R. R. & Gifford, P. Proc. natn. Acad. Sci. U.S.A. 79, 7410–7414 (1982).

    Article  ADS  CAS  Google Scholar 

  57. Sledziewski, A. & Young, E. T. Proc. natn. Acad. Sci. U.S.A. 79, 253–256 (1982).

    Article  ADS  CAS  Google Scholar 

  58. Katinka, M., Vasseur, M., Montreau, N., Yaniv, M. & Blangy, D. Nature 290, 720–722 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  59. Nasmyth, K. A., Tatchell, K., Hall, B. D., Astell, G. & Smith, M. Nature 289, 244–250 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  60. Klar, A. J. S., Strathern, J. N. & Hicks, J. B. Cell 25, 517–524 (1981).

    Article  CAS  PubMed  Google Scholar 

  61. Klar, A. J. S., Fogel, S. & McLeod, K. Genetics 93, 37–50 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  62. Haber, J. E. & George, J. P. Genetics 93, 13–35 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  63. Rine, J., Strathern, J. N., Hicks, J. B. & Herskowitz, I. Genetics 93, 877–901 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  64. Nasmyth, K. A. Cell 30, 567–578 (1982).

    Article  CAS  PubMed  Google Scholar 

  65. Dieckmann, C. L., Pape, L. K. & Tzagoloff, A. Proc. natn. Acad. Sci. U.S.A. 79, 1805–1809 (1982).

    Article  ADS  CAS  Google Scholar 

  66. Johnston, J. & Hopper, J. E. Proc. natn. Acad. Sci. U.S.A. 79, 6971–6975 (1982).

    Article  ADS  CAS  Google Scholar 

  67. Laughon, A. & Gesteland, R. F. Proc. natn. Acad. Sci. U.S.A. 79, 6827–6831 (1982).

    Article  ADS  CAS  Google Scholar 

  68. Losson, R. & Lacroute, F. Molec. gen. Genet. 184, 394–399 (1982).

    Article  Google Scholar 

  69. Langford, C. J., Donath, C., Klinz, F. J. & Gallwitz, D. Cell (in the press).

  70. Pikielny, C., Teem, J. & Rosbash, M. Cell (in the press).

  71. Yang, V. W., Lerner, M. R., Steitz, J. A. & Flint, S. J. Proc. natn. Acad. Sci. U.S.A. 78, 1371–1375 (1981).

    Article  ADS  CAS  Google Scholar 

  72. Lerner, M. R., Boyle, J. A., Mount, S. M., Wolin, S. L. & Steitz, J. A. Nature 283, 220–224 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  73. Rogers, J. & Wall, R. Proc. natn. Acad. Sci. U.S.A. 77, 1877–1881 (1980).

    Article  ADS  CAS  Google Scholar 

  74. Harland, R. & Laskey, R. Cell 21, 761–771 (1980).

    Article  CAS  PubMed  Google Scholar 

  75. Hines, P. J. & Benbow, R. M. Cell. 30, 459–468 (1982).

    Article  CAS  PubMed  Google Scholar 

  76. Stinchcomb, D. T., Thomas, M., Kelly, J., Selker, E. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 77, 4559–4563 (1980).

    Article  ADS  CAS  Google Scholar 

  77. Chan, C. S. M. & Tye, B. K. Proc. natn. Acad. Sci. U.S.A. 77, 6329–6633 (1980).

    Article  ADS  CAS  Google Scholar 

  78. Petes, T. D., Newlon, C. S., Byers, B. & Fangman, W. L. Cold Spring Harb. Symp. quant. Biol. 38, 9–16 (1973).

    Google Scholar 

  79. Fangman, W. L., Hice, K. H. & Chlebowicz-Sledziewska, E. Cell 32, 831–838 (1983).

    Article  CAS  PubMed  Google Scholar 

  80. Kojo, H., Greenberg, B. D. & Sugino, A. Proc. natn. Acad. Sci. U.S.A. 78, 7261–7265 (1981).

    Article  ADS  CAS  Google Scholar 

  81. Celniker, S. E. & Campbell, J. Cell 31, 201–213 (1982).

    Article  CAS  PubMed  Google Scholar 

  82. Jazwinski, S. M. & Edelman, G. M. Proc. natn. Acad. Sci. U.S.A. 79, 3428–3432 (1982).

    Article  ADS  CAS  Google Scholar 

  83. Kuo, C. L. & Campbell, J. L. Proc. natn. Acad. Sci. U.S.A. 79, 4243–4247 (1982).

    Article  ADS  CAS  Google Scholar 

  84. Arendes, J., Kim, K. C. & Sugino, A. Proc. natn. Acad. Sci. U.S.A. 80, 673–677 (1983).

    Article  ADS  CAS  Google Scholar 

  85. Broach, J. et al. Cold Spring Harb. Symp. quant. Biol. 47, 1165–1173 (1982).

    Article  CAS  Google Scholar 

  86. Hereford, L. M., Bromley, S. B. & Osley, M. A. Cell 30, 305–310 (1982).

    Article  CAS  PubMed  Google Scholar 

  87. Osley, M. A. & Hereford, L. M. Proc. natn. Acad. Sci. U.S.A. 79, 7689–7693 (1982).

    Article  ADS  CAS  Google Scholar 

  88. Livingston, D. M. & Kupfer, D. J. molec. Biol. 116, 249–260 (1977).

    Article  CAS  PubMed  Google Scholar 

  89. Zakian, V. A., Brewer, B. J. & Fangman, W. L. Cell 17, 923–934 (1979).

    Article  CAS  PubMed  Google Scholar 

  90. Broach, J. R. & Hicks, J. B. Cell 21, 501–508 (1980).

    Article  CAS  PubMed  Google Scholar 

  91. Newlon, C. S., Debenish, R. J., Suci, D. J. & Roffis, C. J. ICN–UCLA Symp. 21, 501–516 (1981).

    Google Scholar 

  92. Broach, J. R., Guarascio, V. R. & Jayaram, M. Cell 29, 227–234 (1982).

    Article  CAS  PubMed  Google Scholar 

  93. Cameron, J. R., Philippsen, P. & Davis, R. W. Nucleic Acids Res. 4, 1429–1448 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  94. Falco, S. C., Li, Y., Broach, J. R. & Botstein, D. Cell 29, 573–584 (1982).

    Article  CAS  PubMed  Google Scholar 

  95. Holm, C. Cell 29, 585–594 (1982).

    Article  CAS  PubMed  Google Scholar 

  96. Falso, S. C. & Botstein, D. Genetics (in the press).

  97. Murray, A. W. & Szostak, J. W. Nature (in the press).

  98. Dani, G. M. & Zakian, V. A. Proc. natn. Acad. Sci. U.S.A. 80, 3406–3410 (1983).

    Article  ADS  CAS  Google Scholar 

  99. Mann, C. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 80, 228–232 (1983).

    Article  ADS  CAS  Google Scholar 

  100. McClintock, B. Genetics 23, 315–376 (1938).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  101. Fitzgerald-Hayes, M., Buhler, J. M., Cooper, T. & Carbon, J. Molec. cell. Biol. 2, 82–87 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  102. Fitzgerald-Hayes, M., Clarke, L. & Carbon, J. Cell 29, 234–244 (1982).

    Article  Google Scholar 

  103. Bloom, K. S., Fitzgerald-Hayes, M. & Carbon, J. Cold Spring Harb. Symp. quant. Biol. 47, 1175–1185 (1982).

    Article  CAS  Google Scholar 

  104. Bloom, K. S. & Carbon, J. Cell 29, 305–317 (1982).

    Article  CAS  PubMed  Google Scholar 

  105. Szostak, J. Cold Spring Harb. Symp. quant. Biol. 47, 1187–1194 (1982).

    Google Scholar 

  106. Blackburn, E. H. & Gall, J. G. J. molec. Biol. 120, 33–53 (1978).

    Article  CAS  PubMed  Google Scholar 

  107. Orr-Weaver, T., Szostak, J. & Rothstein, R. J. Proc. natn. Acad. Sci. U.S.A. 78, 6354–6358 (1981).

    Article  ADS  CAS  Google Scholar 

  108. Orr-Weaver, T. & Szostak, J. Proc. natn. Acad. Sci. U.S.A. 80, 4417–4421 (1983).

    Article  ADS  CAS  Google Scholar 

  109. Strathern, J. N. et al. Cell 31, 183–192 (1982).

    Article  CAS  PubMed  Google Scholar 

  110. Szostak, J., Orr-Weaver, T., Rothstein, R. & Stahl, F. Cell 33, 25–35 (1983).

    Article  CAS  PubMed  Google Scholar 

  111. Holliday, R. Genet. Res. 5, 282–304 (1964).

    Article  Google Scholar 

  112. Meselson, M. S. & Radding, C. M. Proc. natn. Acad. Sci. U.S.A. 72, 358–362 (1975).

    Article  ADS  CAS  Google Scholar 

  113. Fogel, S., Mortimer, R., Lusnak, K. & Tavares, F. Cold Spring Harb. Symp. quant. Biol. 43, 1325–1341 (1978).

    Google Scholar 

  114. Symington, L. S., Fogarty, L. M. & Kolodner, R. Cell (submitted).

  115. Scherer, S. & Davis, R. W. Science 209, 1380–1384 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  116. Roeder, G. S. & Fink, G. R. Proc. natn. Acad. Sci. U.S.A. 79, 5621–5625 (1982).

    Article  ADS  CAS  Google Scholar 

  117. Potier, S., Winsor, B. & Lacroute, F. Molec. cell. Biol. 2, 1025–1032 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  118. Novick, P., Field, C. & Schekman, R. Cell 21, 205–215 (1980).

    Article  CAS  PubMed  Google Scholar 

  119. Stevens, T., Esmon, B. & Schekman, R. Cell 30, 439–448 (1982).

    Article  CAS  PubMed  Google Scholar 

  120. Henry, S., Donohue, T. & Culbertson, M. Molec. gen. Genet. 143, 5–11 (1975).

    Article  CAS  PubMed  Google Scholar 

  121. Fried, H. M. & Warner, J. R. Nucleic Acids Res. 10, 3133–3148 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  122. Clarke, L. & Carbon, J. Nature 305, 23–28 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

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Struhl, K. The new yeast genetics. Nature 305, 391–397 (1983). https://doi.org/10.1038/305391a0

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