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HLA-D region β-chain DNA endonuclease fragments differ between HLA-DR identical healthy and insulin-dependent diabetic individuals

Nature volume 303, pages 815–817 (1983)Cite this article

Abstract

The human HLA-D histocompatibility region encodes class II antigens each of which consists of two polypeptide chains (α and β) inserted in the plasma membrane. These molecules are implicated in the regulation of the immune response1–3 but several human diseases are also found to be associated with certain HLA-DR antigens4. The occurrence of insulin-dependent (type I) diabetes (IDDM) is strongly associated with HLA-DR3 and/or 4 (ref. 5). The class II antigens, however, show a marked genetic polymorphism1 associated with the β-chains6,7 which seem, from hybridization studies, to be encoded by several genes8,9. We have therefore used the β-chain cDNA probe, pDR-β-1 (refs 8, 10) to test whether there are differences in hybridization pattern between DNA from healthy individuals and diabetic patients, after digestion with restriction endonucleases. Among the HLA-DR 4 and 3/4 individuals, the IDDM patients showed an increased frequency of a PstI 18 kilobase (kb) fragment. A BamHI 3.7 kb fragment, frequent among controls (30–40%), was rarely detected in the IDDM patients (0–2%). These differences may be related to susceptibility to develop the disease.

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Authors and Affiliations

  1. Hagedorn Research Laboratory, DK2820, Gentofte, Denmark

    David Owerbach & Åke Lernmark

  2. Tissue Typing Laboratory, University Hospital (Rigshospitalet), Copenhagen, Denmark

    Per Platz & Lars P. Ryder

  3. Department of Cell Research, The Wallenberg Laboratory, University of Uppsala, S-751, 22, Uppsala, Sweden

    Lars Rask & Per A. Peterson

  4. Department of Pediatrics, University of Linköping, S-581, 85 Linköping, Sweden

    Johnny Ludvigsson

Authors
  1. David Owerbach
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  2. Åke Lernmark
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  3. Per Platz
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  4. Lars P. Ryder
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  5. Lars Rask
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  6. Per A. Peterson
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  7. Johnny Ludvigsson
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Owerbach, D., Lernmark, Å., Platz, P. et al. HLA-D region β-chain DNA endonuclease fragments differ between HLA-DR identical healthy and insulin-dependent diabetic individuals. Nature 303, 815–817 (1983). https://doi.org/10.1038/303815a0

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