Abstract
Like other transforming genes of retro viruses, the v-myc gene of the avian virus, MC29, has a homologue in the genome of normal eukaryotic cells. The human cellular homologue, c-myc, located on human chromosome 8, region q24→qter (refs 1, 2), is translocated into the immunoglobulin heavy-chain locus on human chromosome 14 (ref. 3) in Burkitt's lymphoma1,4,5, suggesting that c-myc has a primary role in transformation of some human haematopoietic cells. In addition, c-myc is amplified in the human promyelocytic leukaemia cell line, HL60 (refs 6, 7) which also contains high levels of c-myc mRNA8. Recently, Colby et al.9 reported the nucleotide sequence of the human c-myc DNA isolated from a genomic recombinant DNA library derived from human fetal liver10. This 4,053-base pair (bp) sequence includes two exons and one intron of the myc gene, and the authors have suggested the existence of a human c-myc mRNA of 2,291 nucleotides that has a coding capacity for a protein of molecular weight (Mr) 48,812. We have approached the problem of accurately defining the characteristics of the human c-myc mRNA and c-myc protein by determining the sequence of the c-myc cDNA isolated from a cDNA library prepared from mRNA of a clone of the K562 human leukaemic cell line11. K562 cells are known to contain c-myc mRNA which is similar in size to the c-myc mRNA of other human cell types8. We report here the sequence of 2,121 nucleotides of a human c-myc mRNA and demonstrate that its 5′ noncoding sequence does not correspond to the sequence of the reported genomic human sequence. However, our data confirm that the intact human c-myc mRNA can encode a 48,812-Mr protein with a sequence identical to that reported by Colby et al.9.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Dalla Favera, R. et al. Proc. natn. Acad. Sci. U.S.A. 79, 7824–7827 (1982).
Neel, B. G., Jhanwar, S. C., Chaganti, R. S. K. & Hayward, W. S. Proc. natn. Acad. Sci. 79, 7842–7846 (1982).
Croce, C. M. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3416–3419 (1979).
Taub, R. et al. Proc. natn. Acad. Sci. U.S.A. 79, 7837–7841 (1982).
Dalla Favera, R., Martinotti, S., Gallo, R. C., Erikson, J. & Croce, C. M. Science 219, 963–967 (1983).
Collins, S. J. & Groudine, M. Nature 298, 679–681 (1982).
Dalla Favera, R., Wong-Staal, F. & Gallo, R. C. Nature 299, 61–63 (1982).
Westin, E. et al. Proc. natn. Acad. Sci. U.S.A. 79, 2490–2494 (1982).
Colby, W., Chen, E., Smith, D. & Levinson, A. Nature 301, 722–725 (1983).
Lawn, R. M., Fritsch, E. F., Parker, R. C., Blake, G. & Maniatis, T. Cell 15, 1157–1174 (1978).
Cioe, L. et al. Cancer Res. 41, 237–243 (1981).
Alitalo, K. et al. Proc. natn. Acad. Sci. U.S.A. 80, 100–104 (1983).
Mount, S. M. Nucleic Acids Res. 10, 459–472 (1982).
Stanton, L. W., Watt, R. & Marcu, K. B. Nature 303, 401–406 (1983).
Hagenbuchle, O. et al. Nature 289, 643–646 (1981).
Hamlyn, P. H., Gait, M. J. & Milstein, C. Nucleic Acids Res. 9, 4485–4494 (1981).
Berger, S. & Birkenmeir, C. Biochemistry 18, 5143–5149 (1979).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).
Retzel, E. F., Collet, M. S. & Faras, A. J. Biochemistry 19, 513–518 (1980).
Monahan, J., McReynolds, L. & O'Malley, B. J. biol. Chem. 251, 7355–7362 (1976).
Hoeijmakers, J., Borst, P., Van Den Burg, J., Weissmann, C. & Cross, G. Gene 8, 391–417 (1980).
Villa-Komaroff, L. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3727–3731 (1978).
Weislander, L. Analyt. Biochem. 98, 305–309 (1979).
Dagert, M. & Ehrlich, S. Gene 6, 23–28 (1979).
Grunstein, M. & Hogness, D. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).
Dalla Favera, R., Wong-Staal, F. & Gallo, R. Nature 299, 61–63 (1982).
Rigby, P., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).
Maniatis, T., Fritsch, E. F. & Sambrook, J. (eds) Molecular Cloning, 324–328 (Cold Spring Harbor Laboratory, New York, 1982).
Messing, J. & Vieira, J. Gene 19, 269–276 (1982).
Messing, J., Crea, R. & Seeburg, P. Nucleic Acids Res. 9, 309–321 (1981).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Watt, R., Stanton, L., Marcu, K. et al. Nucleotide sequence of cloned cDNA of human c-myc oncogene. Nature 303, 725–728 (1983). https://doi.org/10.1038/303725a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/303725a0
This article is cited by
-
Upregulation of a functional form of the β4 integrin subunit in colorectal cancers correlates with c-Myc expression
Oncogene (2005)
-
The Mad1 transcription factor is a novel target of activin and TGF-β action in keratinocytes: possible role of Mad1 in wound repair and psoriasis
Oncogene (2001)
-
Regulation of the expression of c-Myc by β1 integrins in epithelial cells
Oncogene (2001)
-
The amplification of oligonucleotide themes in the evolution of themyc protooncogene family
Journal of Molecular Evolution (1996)
-
Varied expression of major histocompatibility complex and oncogenes in shope carcinoma cell lines derived from a single tumor
Journal of Cancer Research and Clinical Oncology (1995)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.