Abstract
DNA sequences capable of inducing oncogenic transformation of NIH3T3 mouse cells are found in a number of human tumour cell lines. When DNAs of these cell lines are applied to monolayer cultures of the mouse fibroblasts, foci of transformed cells are observed 2–3 weeks later1–5. DNA from cells of such primary foci can be used in turn to induce foci in a second cycle of gene transfer2,3. The human DNA sequences responsible for transformation have been called oncogenes, the best characterized of which is closely related to the Harvey murine sarcoma virus oncogene. Here we present a characterization of an oncogene which we found originally to be present in DNA of the SW480 colon carcinoma cell line. We indicate its structural outlines and demonstrate, in extension of reported results3,4, its presence in an activated form in the genome of several types of human tumour cell lines as well as in biopsy tissue from an adenocarcinoma of the large bowel. We identify this tumour oncogene with c-Ki-ras 2, one of two known members of the Kirsten ras family of human proto-oncogenes, extending a series of recent reports5–7 which have demonstrated homologies between human oncogenes and those of Harvey and Kirsten murine sarcoma viruses. The c-Ki-ras 2 oncogene of several tumour cell lines is shown to be amplified.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Cooper, G. M. Science 217, 801–806 (1982).
Shih, C., Padhy, L. C., Murray, M. J. & Weinberg, R. A. Nature 290, 261–264 (1981).
Murray, M. J. et al. Cell 25, 355–361 (1981).
Perucho, M., Goldfarb, M., Shimizu, K., Lama, C., Fogh, J. & Wigler, M. Cell 27, 467–476 (1981).
Der, C. J., Krontiris, T. G. & Cooper, G. M. Proc. natn. Acad. sci. U.S.A. 79, 3637–3640 (1982).
Parada, L. F., Tabin, C. J., Shih, C. & Weinberg, R. A. Nature 297, 474–478 (1982).
Santos, E., Tronick, S. R., Aaronson, S. A., Pulciani, S. & Barbacid, M. Nature 298, 343–347 (1982).
Tabin, C. J. et al. Nature 300, 143–149 (1982).
Chang, E. H., Gonda, M. A., Ellis, R. W., Scolnick, E. M. & Lowy, D. R. Proc. natn. Acad. Sci. U.S.A. 79, 4848–4852 (1982).
Shih, C. & Weinberg, R. A. Cell 29, 161–169 (1982).
Goldfarb, M., Shimizu, K., Perucho, M. & Wigler, M. Nature 296, 404–409 (1982).
Pulciani, S. et al. Proc. natn. Acad. sci. U.S.A. 79, 2845–2849 (1982).
Dhar, R. et al. Science 217, 934–936 (1982).
Tsuchida, N., Ryder, T. & Ohtsubo, E. Science 217, 936–938 (1982).
Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).
Rubin, C. M., Houck, C. M., Deininger, P. L., Friedmann, T. & Schmid, C. W. Nature 284, 372–374 (1980).
Whang-Peng, J. et al. Cancer Gen. Cytogen. 6, 119–134 (1982).
Perucho, M., Hanahan, D. & Wigler, M. Cell 22, 309–317 (1980).
Southern, E. M. J. molec. Biol. 91, 315–328 (1975).
Ellis, R. W. et al. Nature 292, 506–511 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
McCoy, M., Toole, J., Cunningham, J. et al. Characterization of a human colon/lung carcinoma oncogene. Nature 302, 79–81 (1983). https://doi.org/10.1038/302079a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/302079a0
This article is cited by
-
An Update on the Biology of RAS/RAF Mutations in Colorectal Cancer
Current Colorectal Cancer Reports (2011)
-
Krüppel-like factor 5 is a crucial mediator of intestinal tumorigenesis in mice harboring combined Apc Min and KRASV 12mutations
Molecular Cancer (2010)
-
The COSMIC (Catalogue of Somatic Mutations in Cancer) database and website
British Journal of Cancer (2004)
-
Clinicopathological significance of Ki-ras point mutation and p21 expression in benign and malignant exocrine tumors of the human pancreas
International journal of pancreatology (1996)
-
Neuroendocrine neoplasms of the lung are not associated with point mutations at codon 12 of the Ki-ras gene
Virchows Archiv B Cell Pathology Including Molecular Pathology (1993)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.