Abstract
Human fibroblast cells produce β-type interferon only in response to viral infection or treatment with an inducer such as poly(rI) · poly(rC); this event is most probably controlled at the transcriptional level (for review see ref. 1). To study the induction process, we inserted the human fibroblast interferon (IFN-β) gene, with or without its promoter region, into recombinant simian virus 40 (SV40) plasmid vectors which subsequently were transfected into monkey AP-8 cells. We report here that upon induction with poly(rI) · poly(rC) there was a 10–30-fold increase in IFN-β synthesis. This inducer had no effect on interferon production when the coding region only was inserted into the vector plasmid, which indicates that the promoter region is required for inducibility of this gene. Deletion mapping implicates the region between nucleotides −144 and −186 from the mRNA initiation site in the specific regulation of the IFN-β gene. This region contains a sequence that is remarkably homologous with a consensus sequence found in the 5′ flanking region of steroid hormone responsive genes, which might be involved in binding the progesterone–receptor complex2.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Stewart, W. E. II The Interferon System (Springer, New York, 1979).
Mulvihill, E. R., Le Pennec, J. & Chambon, P. Cell 24, 621–632 (1982).
Nagata, S. et al. Nature 284, 316–320 (1980).
Goeddel, D. V. et al. Nature 290, 20–26 (1981).
Maeda, S. et al. Proc. natn. Acad. Scci. U.S.A. 77, 7010–7013 (1980).
Derynck, R. et al. Nature 285, 542–546 (1980).
Taniguchi, T., Fujii-Kuryama, Y. & Muramatsu, M. Proc. natn. Acad. Sci. U.S.A. 77, 4003–4006 (1980).
Goeddel, D. V., Shepard, H. M., Yelverton, E., Leung, D. & Crea, R. Nucleic Acids Res. 8, 4057–4074 (1980).
Gray, P. W. et al. Nature 295, 503–508 (1982).
Devos, R. et al. Nucleic Acids Res. 10, 2487–2501 (1982).
Nagata, S., Mantei, N. & Weissmann, C. Nature 287, 401–408 (1980).
Lawn, R. M. et al. Proc. natn. Acad. Sci. U.S.A. 78, 5435–5439 (1981).
Tavernier, J., Derynck, R. & Fiers, W. Nucleic Acids Res. 9, 461–471 (1981).
Degrave, W., Derynck, R., Tavernier, J., Haegeman, G. & Fiers, W. Gene 14, 137–143 (1981).
Houghton, M. et al. Nucleic Acids Res. 9, 247–266 (1981).
Ohno, S. & Taniguchi, T. Proc. natn. Acad. Sci. U.S.A. 78, 5305–5309 (1981).
Gross, G. et al. Nucleic Acids Res. 9, 2495–2507 (1981).
Lawn, R. M. et al. Nucleic Acids Res. 9, 1045–1052 (1981).
Mory, Y. et al. Eur. J. Biochem. 120, 197–202 (1981).
Gray, P. W. & Goeddel, D. V. Nature 298, 859–863 (1982).
Taya, Y. et al. EMBO J. 8, 953–958 (1982).
Gheysen, D. & Fiers, W. J. molec. appl. Genet. 1, 385–394 (1982).
Derynck, R. et al. Nature 287, 193–197 (1980).
Ohno, S. & Taniguchi, T. Nucleic Acids Res. 10, 967–977 (1982).
Hauser, H. et al. Nature 297, 650–654 (1982).
Zinn, K., Mellon, P., Ptashne, M. & Maniatis, T. Proc. natn. Acad. Sci. U.S.A. 79, 4897–4901 (1982).
Canaani, D. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 79, 5166–5170 (1982).
Ullrich, A., Gray, A., Goeddel, D. V. & Dull, T. J. J. molec. Biol. 156, 467–486 (1982).
Fiers, W. et al. Phil. Trans. R. Soc. B 299, 29–38 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tavernier, J., Gheysen, D., Duerinck, F. et al. Deletion mapping of the inducible promoter of human IFN-β gene. Nature 301, 634–636 (1983). https://doi.org/10.1038/301634a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/301634a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
