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Eukaryotic RNA polymerase II binds to nucleosome cores from transcribed genes

Nature volume 301, pages 482–488 (1983)Cite this article

Abstract

Purified RNA polymerase II from calf thymus can bind to about 15% of the nucleosome core particles prepared from mouse myeloma cells, forming a discrete complex having a sedimentation coefficient of 18S. These bound nucleosome cores are heavily enriched in transcribed DNA sequences, are deficient in histones H2A and H2B, and undergo a reversible change in structure on RNA polymerase II binding.

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Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    Bradford W. Baer & Daniela Rhodes

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  1. Bradford W. Baer
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  2. Daniela Rhodes
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Baer, B., Rhodes, D. Eukaryotic RNA polymerase II binds to nucleosome cores from transcribed genes. Nature 301, 482–488 (1983). https://doi.org/10.1038/301482a0

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