Abstract
The activation of T lymphocytes for many responses1–4 requires interaction with specialized antigen presenting cells (APC) which express I region associated (Ia) antigens. Cells of the monocyte-macrophage lineage5 and the recently described dendritic cell6 have been shown to be effective APC. Antigen-specific T cells appear to recognize a complex of antigen and Ia molecules on the surface of the APC and such joint recognition is the basis of the restriction of T-cell activation due to the major histocompatibility complex7. The Ia molecules appear to be Ir gene products8–10. However the biochemical nature of antigen presentation has been difficult to approach largely because of problems in obtaining sufficient numbers of highly purified APC. Although several Ia antigen-positive B lymphoma cell lines, which have proved very effective APC, have been recently described11,12, they have all been of the same H–2d haplotype. We report here on hybridomas produced by fusion of normal C57BL/6 (B6) B cells (H–2b) to a hypoxanthine guanosine phosphoribosyl transferase (HGPRT) deficient variant of one of these lymphoma cell lines. The hybridomas appear to express functional Ia molecules of both parental cell haplotypes. Such cloned somatic cell hybrids should prove useful in studying the properties of Ir genes and Ia molecules in antigen presentation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Waldman, H. Immun. Rev. 42, 202–223 (1978).
Weinberger, O., Herrmann, S. H., Mescher, M. F., Benacerraf, B. & Burakoff, S. J. Proc. natn. Acad. Sci. U.S.A. 77, 6091–6095 (1980).
Miller, J. F. A. P., Vadas, M. A., Whitelaw, A. & Gamble, J. Proc. natn. Acad. Sci. U.S.A. 72, 5095–5098 (1975).
Rosenthal, A. S. & Shevach, E. M. J. exp. Med. 138, 1194–1212 (1973).
Rosenthal, A. S. & Shevach, E. M. Contemp. Topics Immunobiol. 5, 47–90 (1976).
Steinman, R. M. & Nussenzweig, M. C. Immun. Rev. 53, 127–147 (1980).
Paul, W. E., Shevach, E. M., Pickeral, S., Thomas, D. W. & Rosenthal, A. S. J. exp. Med. 145, 618–630 (1977).
Lerner, E. A. et al. J. exp. Med. 152, 1085–1101 (1980).
Sredni, B., Matis, L. A., Lerner, E. A., Pual, W. E. & Schwartz, R. H. J. exp. Med. 153, 677–693 (1981).
McKenzie, I. F. C. et al. J. exp. Med. 150, 1323–1338 (1979).
Glimcher, L. H., Kim, K. J., Green, I. & Paul, W. E. J. exp. Med. 155, 445–459 (1982).
McKean, D. J. et al. J. exp. Med. 154, 1419–1431 (1981).
Hamano, T., Kim, K. J., Leiserson, W. & Asofsky, R. J. Immun. (in the press).
Ozato, K., Mayer, N. & Sachs, D. J. Immun. 124, 533–540 (1980).
Jones, P. P., Murphy, D. B. & McDevitt, H. O. J. exp. Med. 148, 925–939 (1978).
Kappler, J. W., Skidmore, B., White, J. & Marrack, P. J. exp. Med. 153, 1198–1214 (1981).
Heber-Katz, E. et al. J. exp. Med. 155, 1086–1098 (1982).
Kimoto, M. & Fathman, C. G. J. exp. Med. 152, 759–770 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Glimcher, L., Hamano, T., Asofsky, R. et al. I region-restricted antigen presentation by B cell–B lymphoma hybridomas. Nature 298, 283–284 (1982). https://doi.org/10.1038/298283a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/298283a0
This article is cited by
-
Dampening of death pathways by schnurri-2 is essential for T-cell development
Nature (2011)
-
Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes
Nature (1986)
-
Functional expression of a transfected murine class II MHC gene
Nature (1983)
-
Accessory cell-dependent selection of specific T-cell functions
Nature (1983)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
