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Malignant transformation of human embryo retinoblasts by cloned adenovirus 12 DNA

Nature volume 298, pages 69–71 (1982)Cite this article

Abstract

Human adenoviruses can morphologically transform rodent cells in vitro. The transforming gene(s) resides in the early region 1 (E1) transcription unit (0–11.2 map units) at the left end of the conventional adenovirus genome1–4. The only adenovirus-transformed human cell line reported so far, the 293 cell line, was isolated from cell cultures transfected with sheared DNA of the non-oncogenic subgroup C adenovirus type 5 (Ad 5)5,6. The ability of this line to complement the growth of Ad 5 E1 mutants has facilitated the isolation of transformation-defective Ad 5 mutants7,8. As no oncogenic subgroup A adenovirus type 12 (Ad 12) transformed human cell lines were available for the selection of Ad 12 E1 mutants, we attempted to isolate such lines by transfecting cell cultures with a recombinant plasmid (pAsc2) which contained the Ad 12 EcoRI-C DNA fragment (0–16.5 map units) inserted at the EcoRI site of pAT153 (ref. 9). We describe here the isolation of a pAsc2-transformed human embryo retinoblast cell line that contains many copies of the Ad 12 transforming region, expresses virus-specified proteins and, following inoculation into athymic mice, forms tumours which resemble retinoblastomas.

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References

  1. Van der Eb, A. J. et al. Cold Spring Harb. Symp. quant. Biol. 44, 383–399 (1980).

    Article  CAS  Google Scholar 

  2. Fujinaga, K. et al. Cold Spring Harb. Symp. quant. Biol. 44, 519–532 (1980).

    Article  CAS  Google Scholar 

  3. Ortin, J., Scheidtmann, K. H., Greenberg, R., Westphal, M. & Doerfler, W. J. Virol. 20, 355–372 (1976).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Wilson, M. C., Fraser, N. W. & Darnell, J. E. Virology 94, 175–184 (1979).

    Article  CAS  Google Scholar 

  5. Graham, F. L. et al. Cold Spring Harb. Symp. quant. Biol. 39 637–650 (1974).

    Article  Google Scholar 

  6. Graham, F. L., Smiley, J., Russell, W. C. & Nairn, R. J. gen. Virol. 36, 59–72 (1977).

    Article  CAS  Google Scholar 

  7. Graham, F. L., Harrison, T. J. & Williams, J. Virology 86, 10–21 (1978).

    Article  CAS  Google Scholar 

  8. Shenk, T. E., Jones, N., Colby, W. & Fowlkes, D. Cold Spring Harb. Symp. quant. Biol. 44, 367–375 (1980).

    Article  CAS  Google Scholar 

  9. Byrd, P. J., Chia, W., Rigby, P. W. J. & Gallimore, P. H. J. gen. Virol. (in the press).

  10. Mukai, N. Prog. Neuropath. 3, 89–128 (1976).

    Google Scholar 

  11. Mukai, N. & Nishida, T. Jap. J. Ophthal. 22, 326–330 (1978).

    Google Scholar 

  12. Mukai, N. et al. Science 210, 1023–1025 (1980).

    Article  ADS  CAS  Google Scholar 

  13. Southern, E. M. J. molec. Biol. 98, 503–518 (1975).

    Article  CAS  Google Scholar 

  14. Gallimore, P. H. & Paraskeva, C. Cold Spring Harb. Symp. quant. Biol. 44, 703–713 (1980).

    Article  Google Scholar 

  15. Paraskeva, C. & Gallimore, P. H. Int. J. Cancer 25, 631–639 (1980).

    Article  CAS  Google Scholar 

  16. Jochemsen, H., Daniels, G. S. G., Lupker, J. H. & van der Eb, A. J., Virology 105, 551–563 (1980).

    Article  CAS  Google Scholar 

  17. Bos, J. L. et al. Cell 27, 121–131 (1981).

    Article  CAS  Google Scholar 

  18. Ts'o, M. O. M. Jap. J. Ophthal. 22, 351–357 (1978).

    Google Scholar 

  19. Ts'o, M. O. M., Fine, B. S. & Zimmermann, L. E. Am. J. Ophthal. 69, 350–359 (1970).

    Article  CAS  Google Scholar 

  20. Mackey, J. K., Rigden, P. M. & Green, M. Proc. natn. Acad. Sci. U.S.A. 73, 4651–4661 (1976).

    Article  ADS  Google Scholar 

  21. Wold, W. S. M., Mackey, J. K., Rigden, P. & Green, M. Cancer Res. 39, 3479–3484 (1979).

    CAS  PubMed  Google Scholar 

  22. Mackey, J. K., Green, M., Wold, W. S. M. & Rigden, P. J. natn. Cancer Inst. 62, 23–26 (1979).

    CAS  Google Scholar 

  23. Green, M., Wold, W. S. M., Mackey, J. K. & Rigden, P. Proc. natn. Acad. Sci. U.S.A. 76, 6606–6610 (1979).

    Article  ADS  CAS  Google Scholar 

  24. Ibelgaufts, H., Jones, K. W., Maitland, N. & Shaw, J. F. Acta neuropath. berl. 56, 113–117 (1982).

    Article  CAS  Google Scholar 

  25. Graham, F. L. & van der Eb, A. J. Virology 52, 456–467 (1973).

    Article  CAS  Google Scholar 

  26. Frost, E. & Williams, J. Virology 91, 39–50 (1978).

    Article  CAS  Google Scholar 

  27. Paraskeva, C., Brown, K. W. & Gallimore, P. H. J. gen Virol. 58, 73–81 (1982).

    Article  Google Scholar 

  28. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  29. Laskey, R. A. Meth. Enzym. 65, 363–371 (1980).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Cancer Research Campaign Laboratories, Department of Cancer Studies, University of Birmingham, The Medical School, Birmingham, B15 2TJ, UK

    P. Byrd, K. W. Brown & P. H. Gallimore

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  1. P. Byrd
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  2. K. W. Brown
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  3. P. H. Gallimore
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Byrd, P., Brown, K. & Gallimore, P. Malignant transformation of human embryo retinoblasts by cloned adenovirus 12 DNA. Nature 298, 69–71 (1982). https://doi.org/10.1038/298069a0

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