Abstract
Immunoglobulin gene expression requires that cells combine the correct genetic information by DNA rearrangement and RNA processing to produce mature, translatable mRNAs. The mechanisms that operate to produce IgD heavy chain (δ chain) mRNA are thought to be especially complex1–3. Like other immunoglobulins, IgD exists in membrane and secreted forms4,5. By analogy with results of studies of μ6–10 and γ11,12 gene expression, we expected that δ chains would also be encoded by two different mRNAs, containing common sequence information transcribed from constant region gene segments but different information transcribed from coding sequences located 3′ to the constant region gene segments. Instead, we identified13 more than two δ mRNAs in normal mouse spleen and in two IgD-secreting plasmacytomas14, TEPC 1017 and TEPC 1033. Now we have used 32P-labelled DNA fragments prepared from a δ cDNA clone15 and a δ genomic clone16 to characterize by hybridization these δ RNAs, fractionated on methyl mercury hydroxide agarose gels. We find that normal mouse spleen contains a major 2.9-kilobase (kb) and a minor 2.1-kb RNA encoding membrane-bound δ chains (δm) and that TEPC 1017 and TEPC 1033 contain similar δm RNAs plus a 1.75-kb RNA encoding secreted δ chains (δm). We have also observed less abundant δ RNA species (2.65 and 3.2 kb). Different gene segments or combinations of segments are used in the 3′ termini of the multiple δ RNAs.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Tucker, P. W., Liu, C.-P., Mushinski, J. F. & Blattner, F. R. Science 209, 1353–1360 (1980).
Moore, K. W. et al. Proc. natn. Acad. Sci. U.S.A. 78, 1800–1804 (1981).
Maki, R. et al. Cell 24, 353–365 (1981).
Melcher, U. et al. J. exp. Med. 140, 1427–1431 (1974).
Finkelman, F. D., Woods, V. L., Berning, A. & Scher, I. J. Immun. 123, 1253–1259 (1979).
Kehry, M., Sibley, C., Fuhrman, J., Schilling, J. & Hood, L. E. Proc. natn. Acad. Sci. U.S.A. 76, 2932–2936 (1979).
Singer, P. A., Singer, H. H. & Williamson, A. R. Nature 285, 294–300 (1980).
Alt, F. W. et al. Cell 20, 293–301 (1980).
Rogers, J. et al. Cell 20, 303–312 (1980).
Early, P. et al. Cell 20, 313–319, (1980).
Tyler, B. M., Cowman, A. F., Adams, J. M. & Harris, A. W. Nature 293, 406–408 (1981).
Rogers, J. et al. Cell 26, 19–27 (1981).
Fitzmaurice, L. et al. in Immunoglobulin Idiotypes and Their Expression (eds Janeway, C., Sercarz, E. E., Wigzell, H. & Fox, C. F.) 263–269 (Academic, New York, 1981).
Finkelman, F. D., Kessler, S. W., Mushinski, J. F. & Potter, M. J. Immun. 126, 680–687 (1981).
Mushinski, J. F. et al. Proc. natn. Acad. Sci. U.S.A. 77, 7405–7409 (1980).
Liu, C.-P., Tucker, P. W., Mushinski, J. F. & Blattner, F. Science 209, 1348–1353 (1980).
Cheng, H.-L., Blattner, F. R., Fitzmaurice, L., Mushinski, J. F. & Tucker, P. W. Nature 296, 410–415 (1982).
Coleclough, C., Cooper, D. & Perry, R. P. Proc. natn. Acad. Sci. U.S.A. 77, 1422–1426 (1980).
Bargellesi, A., Corte, G., Cosulich, E. & Ferrarini, M. Eur. J. Immun. 9, 490–492 (1979).
Scher, I., Berning, A. K., Kessler, S. & Finkelman, F. D. J. Immun. 125, 1686–1693 (1980).
Perry, R. P. & Kelley, D. E. Cell 18, 1333–1339 (1979).
Kemp, D., Harris, A. W. & Adams, J. M. Proc. natn. Acad. Sci. U.S.A. 77, 7400–7404 (1980).
Setzer, D. R., McGrogan, M., Nunberg, J. H. & Schimke, R. T. Cell 22, 361–370 (1980).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
Rigby, P. J. W., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).
Chirgwin, M. J., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979).
Auffray, C. & Rougeon, F. Eur. J. Biochem. 107, 303–314 (1980).
Bailey, J. M. & Davidson, N. Analyt. Biochem. 70, 75–85 (1976).
Alwine, J. C., Kemp, D. J. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).
Wahl, G. M., Stern, M. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 76, 3683–3687 (1979).
Alwine, J. C. et al. Meth. Enzym. 68, 220–242 (1979).
Kemp, D. J., Harris, A. W., Cory, S. & Adams, J. M. Proc. natn. Acad. Sci. U.S.A. 77, 2876–2880 (1980).
Caspar, D. L. D. Adv. Protein Chem. 18, 37–121 (1963).
Lane, L. C. & Kaesberg, P. Nature 232, 40–43 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fitzmaurice, L., Owens, J., Blattner, F. et al. Mouse spleen and IgD-secreting plasmacytomas contain multiple IgD δ chain RNAs. Nature 296, 459–462 (1982). https://doi.org/10.1038/296459a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/296459a0
This article is cited by
-
The molecular biology of immunoglobulin D
Nature (1984)
-
Structure of genes for membrane and secreted murine IgD heavy chains
Nature (1982)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.