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Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site

Nature volume 295, pages 380–384 (1982)Cite this article

Abstract

Early region IA of human adenoviruses encodes a function required for normal induction of early viral genes and virus-induced cell transformation. The region is expressed at early times as two overlapping spliced mRNAs, 12S and 13S, which encode closely related proteins. To distinguish between the functions of these proteins, a single T → G transversion was constructed which prevents splicing of the 12S mRNA. This transversion, in the second base of the 12S mRNA intron, does not alter the protein encoded by the 13S mRNA due to degeneracy in the genetic code. Studies with this mutant demonstrated that only the 13S mRNA encodes the regulatory protein required for normal early gene expression.

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References

  1. Breathnach, R., Benoist, C., O'Hare, K., Gannon, F. & Chambon, P. Proc. Natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).

    Article  ADS  CAS  Google Scholar 

  2. Lerner, M. R., Boyle, J. A., Mount, S. M., Wolin, S. L. & Steitz, J. A. Nature 283, 220–224 (1980).

    Article  ADS  CAS  Google Scholar 

  3. Rogers, J. & Wall, R. Proc. Natn. Acad. Sci. U.S.A. 77, 1877–1879 (1980).

    Article  ADS  CAS  Google Scholar 

  4. Berk, A. J. & Sharp, P. A. Cell 14, 695–711 (1978).

    Article  CAS  Google Scholar 

  5. Berk, A. J., Lee, F., Harrison, T., Williams, J. & Sharp, P. A. Cell 17, 935–944 (1979).

    Article  CAS  Google Scholar 

  6. Jones, N. & Shenk, T. Proc. Natn. Acad. Sci. U.S.A. 76, 3665–3669 (1979).

    Article  ADS  CAS  Google Scholar 

  7. Graham, F. L., Harrison, T. & Williams, J. Virology 86, 10–21 (1978).

    Article  CAS  Google Scholar 

  8. Jones, N. & Shenk, T. Cell 17, 683–689 (1979).

    Article  CAS  Google Scholar 

  9. Perricaudet, M., Akusjarvi, G., Virtanen, A. & Petterson, U. Nature 281, 694–696 (1979).

    Article  ADS  CAS  Google Scholar 

  10. Baker, C. C. & Ziff, E. B. J. Molec. Biol. 149, 189–221 (1981).

    Article  CAS  Google Scholar 

  11. Harrison, T., Graham, F. & Williams, J. Virology 77, 319–329 (1977).

    Article  CAS  Google Scholar 

  12. Ricciardi, R. P., Jones, R. L., Cepko, C. L., Sharp, P. A. & Roberts, B. E. Proc. Natn. Acad. Sci. U.S.A. 78, 6121–6125 (1981).

    Article  ADS  CAS  Google Scholar 

  13. Razin, A., Hirose, T., Itakura, K. & Riggs, A. Proc. Natn. Acad. Sci. U.S.A. 75, 4268–4270 (1978).

    Article  ADS  CAS  Google Scholar 

  14. Hutchison, C. A. et al. J. biol. Chem. 253, 6551–6560 (1978).

    CAS  Google Scholar 

  15. Gillam, S., Astell, C. R. & Smith, M. Gene 12, 129–137 (1980).

    Article  CAS  Google Scholar 

  16. Matteucci, M. D. & Caruthers, M. H. J. Am. chem. Soc. 103, 3185–3191 (1981).

    Article  CAS  Google Scholar 

  17. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  18. Smith, A. J. H. Meth. Enzym. 65, 560–580 (1980).

    Article  ADS  CAS  Google Scholar 

  19. Stow, N. D. J. Virol. 37, 171–180 (1981).

    CAS  PubMed  PubMed Central  Google Scholar 

  20. Graham, F. L., Smiley, J., Russell, W. C. & Nairn, R. J. gen. Virol. 36, 59–72 (1977).

    Article  CAS  Google Scholar 

  21. Aiello, L., Huebner, K. & Weinmann, R. Virology 94, 460–469 (1979).

    Article  CAS  Google Scholar 

  22. Lassam, N. J., Bayley, S. T. & Graham, F. L. Cold Spring Harb. Symp. Quant. Biol. 44, 477–491 (1980).

    Article  CAS  Google Scholar 

  23. Berk, A. J. & Sharp, P. A. Cell 12, 721–732 (1977).

    Article  CAS  Google Scholar 

  24. Sambrook, J. et al. Cold Spring Harb. Symp. Quant. Biol. 44, 569–584 (1980).

    Article  CAS  Google Scholar 

  25. Spector, D. J., McGrogan, M. & Raskas, H. J. J. Molec. Biol. 126, 395–414 (1978).

    Article  CAS  Google Scholar 

  26. Chow, L. T., Broker, T. R. & Lewis, J. B. J. Molec. Biol. 134, 265–303 (1979).

    Article  CAS  Google Scholar 

  27. Frost, E. & Williams, J. Virology 91, 39–50 (1978).

    Article  CAS  Google Scholar 

  28. Solnick, D. Nature 291, 508–510 (1981).

    Article  ADS  CAS  Google Scholar 

  29. Baird, M. et al. Proc. natn. Acad. Sci. U.S.A. 78, 4218–4221 (1981).

    Article  ADS  CAS  Google Scholar 

  30. Shander, M., Woude, S. V., Proudfoot, N. & Maniatis, T. J. supramolec. Struct. Cell Biochem. (Suppl.) 5, 229 (1981).

    Google Scholar 

  31. Ohshima, Y., Itoh, M., Okada, N. & Miyata, T. Proc. natn. Acad. Sci. U.S.A. 78, 4471–4474 (1981).

    Article  ADS  CAS  Google Scholar 

  32. Yang, V. W., Lerner, M. R., Steitz, J. A. & Flint, S. J. Proc. natn. Acad. Sci. U.S.A. 78, 1371–1375 (1981).

    Article  ADS  CAS  Google Scholar 

  33. Peebles, C. L., Ogden, R. C., Knapp, G. & Abelson, J. Cell 18, 27–37 (1979).

    Article  CAS  Google Scholar 

  34. Dijkema, R. et al. Gene 12, 287–299 (1980).

    Article  CAS  Google Scholar 

  35. Sawada, Y. & Fujinaga, K. J. Virol. 36, 639–651 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  36. van Ormondt, H., Maat, J., De Waard, A. & van der Eb, A. J. Gene 4, 309–328 (1978).

    Article  CAS  Google Scholar 

  37. Osborne, T. F., Schell, R. E., Burch-Jaffe, E., Berget, S. J. & Berk, A. J. Proc. natn. Acad. Sci. U.S.A. 78, 1381–1385 (1981).

    Article  ADS  CAS  Google Scholar 

  38. Hines, J. C. & Ray, D. S. Gene 11, 207–218 (1980).

    Article  CAS  Google Scholar 

  39. Messing, J. Recombinant DNA tech. Bull. 2, 43–48 (1979).

    CAS  Google Scholar 

  40. Jay, E., Bambara, R., Padmanabham, R. & Wu, R. Nucleic Acids Res. 1, 331–353 (1973).

    Article  Google Scholar 

  41. Bolivar, F. et al. Gene 2, 95–113 (1977).

    Article  CAS  Google Scholar 

  42. van der Eb, A. J. & Graham, F. L. Meth. Enzym. 65, 826–839 (1980).

    Article  CAS  Google Scholar 

  43. Anderson, S. Nucleic Acids Res. 9, 3015–3027 (1981).

    Article  CAS  Google Scholar 

  44. Dunsworth-Browne, M., Schell, R. E. & Berk, A. J. Nucleic Acids Res. 8, 543–554 (1980).

    Article  CAS  Google Scholar 

  45. Nomura, N. & Ray, D. A. Proc. Natn. Acad. Sci. U.S.A. 77, 6566–6570 (1980).

    Article  ADS  CAS  Google Scholar 

  46. Vogt, V. M. Eur. J. Biochem. 33, 192–200 (1973).

    Article  CAS  Google Scholar 

  47. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology and Molecular Biology Institute, University of California, Los Angeles, California, 90024, USA

    Craig Montell & Arnold J. Berk

  2. Department of Chemistry, University of Colorado, Campus Box 215, Boulder, Colorado, 80309, USA

    Eric F. Fisher & Marvin H. Caruthers

Authors
  1. Craig Montell
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  2. Eric F. Fisher
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  3. Marvin H. Caruthers
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  4. Arnold J. Berk
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Montell, C., Fisher, E., Caruthers, M. et al. Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site. Nature 295, 380–384 (1982). https://doi.org/10.1038/295380a0

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