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Long-term growth and cloning of non-transformed lymphocytes

Abstract

The diversity of lymphocytes has made it difficult to study the properties of a specific lymphocyte type. Here we describe methods used in long-term culture and cloning of T lymphocytes and discuss the potential uses of these cell lines in immunology.

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References

  1. Ben-Sasson, S. Z., Paul, W. E., Shevach, E. & Green, I. J. exp. Med. 141, 90–105 (1975).

    Article  Google Scholar 

  2. MacDonald, H. R., Engers, H. D., Cerottini, J.-C. & Brunner, K. J. exp. Med. 140, 718–730 (1974).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Morgan, D. A., Ruscetti, F. W. & Gallo, R. Science 193, 1007–1008 (1976).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Möller, G. (ed) Immun. Rev. 51, 1–357 (1980).

  5. Nabholz, M., Engers, H. D., Collavo, D. & North, M. Curr. Topics Microbiol. Immun. 81, 176–187 (1978).

    Google Scholar 

  6. Fathman, C. G. & Hengartner, H. Nature 272, 617–618 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Kimoto, M. & Fathman, C. G. J. exp. Med. 152, 759–770 (1980).

    Article  CAS  PubMed  Google Scholar 

  8. Sredni, B., Tse, H. Y. & Schwartz, R. H. Nature 283, 581–583 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Nabel, G., Fresno, M., Chessman, A. & Cantor, H. Cell. 23, 19–28 (1981).

    Article  CAS  PubMed  Google Scholar 

  10. Sredni, B., Tse, H. Y., Chen, C. & Schwartz, R. H. J. Immun. 126, 341–347 (1981).

    CAS  PubMed  Google Scholar 

  11. Farrar, J. J., Mizel, S. B., Fuller-Farrar, J., Farrar, W. L. & Hilfilker, M. L. J. Immun. 125, 793–798 (1980).

    CAS  PubMed  Google Scholar 

  12. Larson, E. L., Iscove, N. N. & Countinho, A. Nature 283, 664–666 (1980).

    Article  ADS  Google Scholar 

  13. Mizel, S. B. & Mizel, D. J. Immun. 126, 834–837 (1981).

    CAS  PubMed  Google Scholar 

  14. Rosenwasser, L. J., Dinarello, C. A. & Rosenthal, A. S. J. exp. Med. 150, 709–713 (1979).

    Article  CAS  PubMed  Google Scholar 

  15. Schmidt, J. A., Mizel, S. B. & Green, I. Fedn Proc. 40(3), 1084 (1981).

    Google Scholar 

  16. Smith, K. A. Immun. Rev. 51, 337–357 (1980).

    Article  CAS  PubMed  Google Scholar 

  17. Robb, R. J., Munck, A. & Smith, K. A. J. exp. Med. 154, 1455–1474 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Gillis, S., Smith, K. A. & Watson, J. J. Immun. 124, 1954–1962 (1980).

    CAS  PubMed  Google Scholar 

  19. Farrar, J. et al. J. Immun. 125, 2555–2558 (1980).

    CAS  PubMed  Google Scholar 

  20. Watson, J., Gillis, S., Marbrook, J., Mochizuki, D. & Smith, K. A. J. exp. Med. 150, 849–861 (1979).

    Article  CAS  PubMed  Google Scholar 

  21. Piperno, A. G., Vassalli, J. & Reich, E. J. exp. Med. 154, 422–431 (1981).

    Article  Google Scholar 

  22. Paul, W. E. & Benacerraf, B. Science 195, 1293–1300 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  23. Sredni, B., Volkman, D., Schwartz, R. H. & Fauci, A. S. Proc. natn. Acad. Sci. U.S.A. 78, 1858–1862 (1981).

    Article  ADS  CAS  Google Scholar 

  24. Sredni, B., Matis, L. A., Paul, W. E. & Schwartz, R. H. J. exp. Med. 153, 677–693 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Fathman, C. G. & Kimoto, M. Immun. Rev. 51, 57–80 (1981).

    Article  Google Scholar 

  26. Jones, P. P., Murphy, D. B. & McDevitt, H. O. J. exp. Med. 148, 925–939 (1978).

    Article  CAS  PubMed  Google Scholar 

  27. von Boehmer, H. et al. J. Immun. 9, 592–597 (1979).

    CAS  Google Scholar 

  28. Sredni, B. & Schwartz, R. H. Nature 287, 855–857 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  29. Schwartz, R. H. & Sredni, B. Isolation, Characterization and Utilization of T Lymphocytes (eds Fathman, G. & Fitch, F. W.) (Academic, New York, in the press).

  30. Howard, M., Kessler, S., Chused, T. & Paul, W. E. Proc. natn. Acad. Sci. U.S.A. 78, 5788–5792 (1981).

    Article  ADS  CAS  Google Scholar 

  31. Sredni, B., Sieckmann, D. G., Kumagai, S. H., Green, I. & Paul, W. E. J. exp. Med. 154, 1500–1516 (1981).

    Article  CAS  PubMed  Google Scholar 

  32. Bach, F. H., Alter, B. J., Widmer, M. B., Segall, M. & Dunlap, B. Immun. Rev. 54, 5–26 (1981).

    Article  CAS  PubMed  Google Scholar 

  33. Lotze, M. T., Line, B. R., Mathisen, D. J. & Rosenberg, S. A. J. Immun. 125, 1487–1493 (1980).

    CAS  PubMed  Google Scholar 

  34. Gillis, S. & Watson, J. Immun. Rev. 54, 81–110 (1981).

    Article  CAS  PubMed  Google Scholar 

  35. Gootenberg, J. E., Ruscetti, F. W., Mier, J. W., Gazdar, A. & Gallo, R. C. J. exp. Med. 154, 1403–1418 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Paul, W., Sredni, B. & Schwartz, R. Long-term growth and cloning of non-transformed lymphocytes. Nature 294, 697–699 (1981). https://doi.org/10.1038/294697a0

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