Abstract
Our understanding of the mechanism of T-cell help in antibody production in the mouse has been improved by the production of long-term lines of T cells, either using T-cell growth factor (TCGF or IL-2) or by creating T-cell hybridomas. Such cells have been shown to produce non-antigen-specific1,2 or antigen-specific helper factors3 with genetically restricted or unrestricted activity1–3. However, the factors are still not well characterized. We have examined the role of human T-helper cells and their soluble factors in inducing antibody production, initially in a xenogeneic system, using mouse B cells4. Using a human in vitro secondary antibody response system for influenza virus5 we have investigated the effect of helper factors on human B cells. We report here the production of long-term cultures of antigen-specific human helper cells. Both the cells and the high-potency antigen-specific helper factor (HF) which they produce act on human B cells in a genetically restricted manner. Preliminary mapping of the restriction indicates associations, but not identity, with HLA-DR.
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References
Schreier, M. H., Iscove, N. N., Tees, R., Aarden, L. & von Boehmer, H. Immun. Rev. 51, 315–336 (1980).
Watson, J. J. exp. Med. 150, 1510–1519 (1979).
Eshar, Z. et al. Nature 286, 270–272 (1980).
Kantor, F. & Feldmann, M. Clin. exp. Immun. 36, 71–77 (1979).
Callard, R. E. Nature 282, 734–736 (1979).
Abramson, C. S., Kersey, J. H. & Le Bien, T. J. Immun. 126, 83–88 (1981).
Tees, R. & Schreier, M. H. Nature 283, 780–781 (1980).
Feldmann, M. & Kontiainen, S. Lymphokines 2, 87–123 (1981).
Shiozawa, C., Singh, B., Robinstein, S. & Diener, E. J. Immun. 118, 2199–2206 (1977).
Lonai, P., Puri, J. & Hammerling, G. J. Proc. natn. Acad. Sci. U.S.A. (in the press).
Doran, T. J., Honeyman, M., Zwinge, W., Edwards, C. M. & Basher, H. W. 8th Int. Workshop on Histocompatibility, 808–809 (ed. Terasaki, P. I.) (UCLA Tissue Typing Laboratory, Los Angeles, 1980).
Bodmer, W. Tissue Antigens 17, 9–20 (1981).
Munro, A. J. & Taussig, M. S. Nature 256, 103–196 (1975).
Nadler, L. M. et al. Nature 290, 591–593 (1981).
Friedmann, S. M., Hunter, S. B., Thomas, Y. & Chess, L. J. Immun. 125, 2202–2207 (1980).
Geha, R. S. Immun. Rev. 45, 275–305 (1979).
Kurnick, J. T. et al. Scand J. Immun. 11, 131–136 (1980).
Beverley, P. C. L. & Callard, R. E. Eur. J. Immun. 11, 329–334 (1981).
Brodsky, F. M., Parham, P., Barnstaple, C. J., Crumpton, M. J. & Bodmer, W. F. Immun. Rev. 47, 3 (1979).
Zanders, E. D., Smith, C. M. & Callard, R. E. J. immun. Meth. (in the press).
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Fischer, A., Beverley, P. & Feldmann, M. Long-term human T-helper lines producing specific helper factor reactive to influenza virus. Nature 294, 166–168 (1981). https://doi.org/10.1038/294166a0
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DOI: https://doi.org/10.1038/294166a0
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